Molecular Definition of Vaginal Microbiota in East African Commercial Sex Workers

Schellenberg, John; Links, Matthew G.; Hill, Janet E.; Dumonceaux, Tim J.; Kimani, Joshua; Jaoko, Walter; Wachihi, Charles; Mungai, Jane Njeri; Peters, Geoffrey A.; Tyler, Shaun; Graham, Morag; Severini, Alberto; Fowke, Keith R.; Ball, T. Blake; Plummer, Francis A.

doi:10.1128/aem.02943-10

Cited by 73 publications

(101 citation statements)

References 55 publications

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“…In the last decade, phylogenetic analyses of cervicovaginal samples (mostly bacterial 16S ribosomal RNA gene sequencing) have shown that bacterial communities in the vagina are more complex than previously thought (Hummelen et al, 2010;Ling et al, 2010;Ravel et al, 2011;Schellenberg et al, 2011;Srinivasan et al, 2012). American studies identified five microbiome clusters (dominated by Lactobacillus iners, L. crispatus, L. gasseri, L. jensenii and a mixture of anaerobic bacteria, respectively) in asymptomatic women (Ravel et al, 2011;Gajer et al, 2012) and multiple clusters consisting of mixtures of anaerobic bacteria, not dominated by a single taxon, in women with BV (Srinivasan et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Lactobacillus-dominated cervicovaginal microbiota associated with reduced HIV/STI prevalence and genital HIV viral load in African women

Tsivtsivadze²,

et al. 2014

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Cervicovaginal microbiota not dominated by lactobacilli may facilitate transmission of HIV and other sexually transmitted infections (STIs), as well as miscarriages, preterm births and sepsis in pregnant women. However, little is known about the exact nature of the microbiological changes that cause these adverse outcomes. In this study, cervical samples of 174 Rwandan female sex workers were analyzed cross-sectionally using a phylogenetic microarray. Furthermore, HIV-1 RNA concentrations were measured in cervicovaginal lavages of 58 HIV-positive women among them. We identified six microbiome clusters, representing a gradient from low semi-quantitative abundance and diversity dominated by Lactobacillus crispatus (cluster R-I, with R denoting 'Rwanda') and L. iners (R-II) to intermediate (R-V) and high abundance and diversity (R-III, R-IV and R-VI) dominated by a mixture of anaerobes, including Gardnerella, Atopobium and Prevotella species. Women in cluster R-I were less likely to have HIV (P ¼ 0.03), herpes simplex virus type 2 (HSV-2; Po0.01), and high-risk human papillomavirus (HPV; Po0.01) and had no bacterial STIs (P ¼ 0.15). Statistically significant trends in prevalence of viral STIs were found from low prevalence in cluster R-I, to higher prevalence in clusters R-II and R-V, and highest prevalence in clusters R-III/R-IV/R-VI. Furthermore, only 10% of HIV-positive women in clusters R-I/R-II, compared with 40% in cluster R-V, and 42% in clusters R-III/ R-IV/R-VI had detectable cervicovaginal HIV-1 RNA (P trend ¼ 0.03). We conclude that L. crispatusdominated, and to a lesser extent L. iners-dominated, cervicovaginal microbiota are associated with a lower prevalence of HIV/STIs and a lower likelihood of genital HIV-1 RNA shedding.

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Section: Introductionmentioning

confidence: 99%

Lactobacillus-dominated cervicovaginal microbiota associated with reduced HIV/STI prevalence and genital HIV viral load in African women

Tsivtsivadze²,

et al. 2014

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“…J. Schellenberg et al (2011) по результатам своей работы сформулировали концепцию, заключа-ющуюся в том, что доминирование G. vaginalis в микробиоте влагалища -это переходное со-стояние между физиологическим, представлен-ным лактобациллами, микробиоценозом и БВ, характеризуемым кодоминированием гардне-релл, бактероидов и клостридий [23].…”

Section: обсуждение результатовunclassified

“…Известно, что бактероиды (в первую очередь, превотеллы) имеют вы-сокие показатели распространенности и ко-ОРИГИНАЛЬНЫЕ ИССЛЕДОВАНИЯ / ORIGINAL RESEARCHES личественного содержания при БВ. Однако с при менением технологий глубокого секве-нирования ДНК было показано, что P. bivia не единственный распространенный при БВ вид превотелл, часто также выявляются P. timonensis, P. amnii, P. melaninogenica, P. disiens [15,23]. Так, в работе J. Shellenberg et al (2011) самым распространенным видом превотелл были P. timonensis, встречавшиеся практически у всех женщин и отвечавшие за 8 % общего содержания бактерий во всех исследованных образцах [23].…”

Section: обсуждение результатовunclassified

“…Однако с при менением технологий глубокого секве-нирования ДНК было показано, что P. bivia не единственный распространенный при БВ вид превотелл, часто также выявляются P. timonensis, P. amnii, P. melaninogenica, P. disiens [15,23]. Так, в работе J. Shellenberg et al (2011) самым распространенным видом превотелл были P. timonensis, встречавшиеся практически у всех женщин и отвечавшие за 8 % общего содержания бактерий во всех исследованных образцах [23]. В исследовании S. Srinivasan et al (2012) P. timonensis, P. bivia, P. amnii вы-являлись у 76, 64, 50 % женщин с БВ соответ-ственно, а совокупный вклад этих трех видов в общее содержание бактерий у женщин с БВ составлял 12 %, по 4 % каждого вида [15].…”

Section: обсуждение результатовunclassified

“…Для сравнения: G. vaginalis встречались у 98 % жен-щин с БВ с показателем содержания 14 %. Что касается другого представителя BacteroidalesPorphyromonas spp., наиболее часто при БВ встречаются виды P. asaccharolytica и P. uenonis, и их распространенность и количественное содержание су щественно ниже соответствую-щих показателей для превотелл [15,23]. Эти данные, в совокупности с хорошо задокумен-тированным фактом практически повсемест-ного присутствия G. vaginalis во влагалище у женщин с БВ, дают основания полагать, что в большинстве случаев при выявлении группы бактерий Gardnerella vaginalis/Prevotella bivia/ Porphyromonas доминирующими бактериями были G. vaginalis.…”

Section: обсуждение результатовunclassified

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Bacterial communities forming the vaginal micro-ecosystem in norm and in bacterial vaginosis

et al. 2017

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Background. Bacterial vaginosis (BV) is disturbance of the vaginal microbiota, characterized by displacement of lactobacilli with anaerobic bacteria and capable of adversely affecting women’s reproductive health. In the development of BV, a wide spectrum of bacteria substantially differing in their properties is involved. Grouping vaginal bacterial communities into clusters, or types of microbiocenosis, might contribute to understanding of pathogenic mechanisms and elaboration of effective tools for diagnostics and therapy of the disease. Aim. Determination and comparative analysis of clusters of vaginal bacterial communities in norm and in BV. Materials and methods. Women of reproductive age were enrolled in the study. For the diagnosis of BV, the Nugent score was used. Vaginal swab samples from all women were analyzed with the test Femoflor-16, intended for evaluation of the vaginal microbiocenosis using multiplex quantitative real-time PCR. Two-step cluster analysis was applied for grouping bacterial communities. Differences between the clusters were evaluated using pairwise comparisons. Results. Of 280 women enrolled in the study, 172 had normal microflora, 27 – intermediate microflora, 81 – BV. In cluster analysis, 270 samples valid in PCR testing were included. All the vaginal bacterial communities were grouped into 4 clusters. Cluster 1 (n = 171) included cases when the vaginal microflora consisted mostly of lactobacilli. Cluster 2 (n = 11) encompassed cases of domination of aerobic microflora: Enterobacteriaceae, Streptococcus and Staphylococcus. Clusters 3 (n = 57) and 4 (n = 31) were connected with BV and included cases of prevailing of facultative anaerobes (Gardnerella vaginalis, Atopobium vaginae) and obligate anaerobes (Sneathia/Leptotrichia/Fusobacterium, Megasphaera/Veillonella/Dialister, Lachnobacterium/Clostridium), respectively. Nearly all cases of cluster 1 belonged to the category of normal microflora of the Nugent score. The majority of bacterial communities of cluster 2 matched intermediate microflora, cluster 3 – BV category with a score of 7 or 8, cluster 4 – BV category with a score of 9 or 10. The clusters differed significantly in vaginal рН, with the highest values observed for cluster 4. Conclusions. Vaginal bacterial communities are grouped into 4 main clusters, characterized by domination of lactobacilli, aerobes, facultative anaerobes or obligate anaerobes. The clusters belong to different categories of the Nugent score and differ significantly in vaginal pH.

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Unraveling the Rhizosphere Using thecpn60 Genomic Marker and Pyrosequencing

Lazarovits¹,

Turnbull²,

Haug

et al. 2013

Molecular Microbial Ecology of the Rhizosphere

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Molecular Definition of Vaginal Microbiota in East African Commercial Sex Workers

Cited by 73 publications

References 55 publications

Lactobacillus-dominated cervicovaginal microbiota associated with reduced HIV/STI prevalence and genital HIV viral load in African women

Lactobacillus-dominated cervicovaginal microbiota associated with reduced HIV/STI prevalence and genital HIV viral load in African women

Bacterial communities forming the vaginal micro-ecosystem in norm and in bacterial vaginosis

Unraveling the Rhizosphere Using thecpn60 Genomic Marker and Pyrosequencing

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