Molecular Design of Photosynthesis-Elevated Chloroplasts for Mass Accumulation of a Foreign Protein

Yabuta, Yukinori; Tamoi, Masahiro; Yamamoto, Kumiko; Tomizawa, Kenji; Yokota, Akiho; Shigeoka, Shigeru

doi:10.1093/pcp/pcn014

Cited by 40 publications

(33 citation statements)

References 21 publications

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“…Moreover, using of a promoter driven by a naturally available stimulus is eco-friendly and does not change the circadian expression of genes of microalgae [15]. The P psbA1 promoter from other sources has earlier been used for expression of desired genes in Anabaena [16] and in other cyanobacteria [17] and plants [18]. Our findings indicate that the pAnFP vector bearing heterologous genes can be successfully expressed in E. coli and be used for transformation of cyanobacteria such as Anabaena or Nostoc strains in the future.…”

Section: Discussionmentioning

confidence: 99%

Construction of a Shuttle Vector for Heterologous Gene Expression in Escherichia coli and Microalgae Anabaena

Song¹,

Li²,

Hu³

et al. 2013

ENG

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The construction of an integrative shuttle expression vector and potential utility was reported in Escherichia coli and Anabaena (Nostoc) sp. strain PCC 7120. The vector comprised of the following elements: (a) an intergenic non-coding region from Anabaena to facilitate its genomic integration (b) a strong functional P psbAI promoter from Anabaena for desired gene expression and (c) neomycin phosphotransferase gene with its own promoter for the selection of transformants. The constructed vector pAnFP was evaluated by cloning, transfer and expression of the gfp gene encoding green fluorescent protein. When the E. coli and Anabaena sp. strain PCC 7120 were transformed, intensive green fluorescence produced by the products of GFP protein was observed. This result indicated that the integrative shuttle vector pAnFP can be promisingly used in genome transformation for expression of heterologous genes in E. coli and microalgae such as Anabaena and Nostoc strains.

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Section: Discussionmentioning

confidence: 99%

Construction of a Shuttle Vector for Heterologous Gene Expression in Escherichia coli and Microalgae Anabaena

Song¹,

Li²,

Hu³

et al. 2013

ENG

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“…On the basis of these results, transplastomic tobacco (Yabuta et al 2008) and lettuce plants (Ichikawa et al 2010) expressing cyanobacterial fructose-1,6/ sedoheptulose-1,7-bisphosphatase (FBP/SBPase) in the chloroplast have recently been generated, and both exhibited increased productivity. In tobacco plants, expression by three different plastid promoters caused a minimum of 2-or 3-fold increase in the enzyme activity, which resulted in up to 1.8-fold increase of the dry matter when compared to the nontransformed plants.…”

Section: Attempts To Influence Crop Productivitymentioning

confidence: 99%

“…In tobacco plants, expression by three different plastid promoters caused a minimum of 2-or 3-fold increase in the enzyme activity, which resulted in up to 1.8-fold increase of the dry matter when compared to the nontransformed plants. This increase was sufficient to improve productivity and photosynthesis without representing a strong metabolic burden to the plastid and potentially enabled the simultaneous high-level expression of other foreign genes in the plastids (Yabuta et al 2008). In addition, the hexose, sucrose, and starch contents of the stem, root, and different leaves were in general higher in transplastomic plants than in non-transformed plants (at least during distinct stages of the diurnal cycle) (Yabuta et al 2008).…”

Section: Attempts To Influence Crop Productivitymentioning

confidence: 99%

“…This increase was sufficient to improve productivity and photosynthesis without representing a strong metabolic burden to the plastid and potentially enabled the simultaneous high-level expression of other foreign genes in the plastids (Yabuta et al 2008). In addition, the hexose, sucrose, and starch contents of the stem, root, and different leaves were in general higher in transplastomic plants than in non-transformed plants (at least during distinct stages of the diurnal cycle) (Yabuta et al 2008). In lettuce, photosynthetic capacity and productivity of the transplastomic plants increased 1.3-and 1.6-fold, respectively, when compared to non-transformed plants (Ichikawa et al 2010).…”

Section: Attempts To Influence Crop Productivitymentioning

confidence: 99%

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Transplastomic plants for innovations in agriculture. A review

Wani

Sah

Sági

et al. 2015

Agron. Sustain. Dev.

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Food production has to be significantly increased in order to feed the fast growing global population estimated to be 9.1 billion by 2050. The Green Revolution and the development of advanced plant breeding tools have led to a significant increase in agricultural production since the 1960s. However, hundreds of millions of humans are still undernourished, while the area of total arable land is close to its maximum utilization and may even decrease due to climate change, urbanization, and pollution. All these issues necessitate a second Green Revolution, in which biotechnological engineering of economically and nutritionally important traits should be critically and carefully considered. Since the early 1990s, possible applications of plastid transformation in higher plants have been constantly developed. These represent viable alternatives to existing nuclear transgenic technologies, especially due to the better transgene containment of transplastomic plants. Here, we present an overview of plastid engineering techniques and their applications to improve crop quality and productivity under adverse growth conditions. These applications include (1) transplastomic plants producing insecticidal, antibacterial, and antifungal compounds. These plants are therefore resistant to pests and require less pesticides. (2) Transplastomic plants resistant to cold, drought, salt, chemical, and oxidative stress. Some pollution tolerant plants could even be used for phytoremediation. (3) Transplastomic plants having higher productivity as a result of improved photosynthesis. (4) Transplastomic plants with enhanced mineral, micronutrient, and macronutrient contents. We also evaluate field trials, biosafety issues, and public concerns on transplastomic plants. Nevertheless, the transplastomic technology is still unavailable for most staple crops, including cereals. Transplastomic plants have not been commercialized so far, but if this crop limitation were overcome, they could contribute to sustainable development in agriculture.

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“…Tobacco leaf accumulated green fluorescent protein of 30% of total soluble proteins when its gene was introduced into the chloroplast genome. 8 Chloroplast transformation takes place by particle bombardment of gold particle coated by vectors against green organelle, and regeneration of transformed plants successfully takes place in several plants, such as tobacco, potato and lettuce.…”

Section: Chloroplast Transformationmentioning

confidence: 99%

Molecular farming: production of drugs and vaccines in higher plants

Shinmyo

Kato

2010

J Antibiot

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On the basis of developments in plant biotechnology, drug and vaccine production by higher plants can be added to microbial and animal cell culture processes. When genes encoding drug or vaccine formation under a suitable promoter are introduced into plants, these useful compounds can be economically produced from CO 2 and inorganic chemicals using sunlight. The merits and demerits of the plant process are discussed in this paper. Keywords: chloroplast transformation; glycosylation; medicinal protein; molecular farming; plant; Prof. Demain; transgene expression Although plants have been used for medicinal purposes for thousands of years, chemical and microbial medicines are being consumed more at present. Recombinant DNA technology in higher plants has opened up a new field of basic research and application in plant science. Basically, foreign genes can be introduced into any kind of plant and it is possible to produce therapeutic proteins, such as antibodies, blood products, cytokines, growth factors, hormones, vaccines and enzymes. The first recombinant plant-derived pharmaceutical protein was human serum albumin discovered in 1990 in transgenic tobacco and potato plants. 1 From an economic point of view, the production cost of biomolecules using plants is less than that of microbial and animal cell cultures, which require equipment, substrate and electric energy supply. Plants can synthesize any protein and metabolite from CO 2 and inorganic chemicals using solar energy. The production cost of immunoglobulin A by transgenic plants is estimated to be less than 1% of that by mammalian cell culture, and less than 5% of that by transgenic goats. 2

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Molecular Design of Photosynthesis-Elevated Chloroplasts for Mass Accumulation of a Foreign Protein

Cited by 40 publications

References 21 publications

Construction of a Shuttle Vector for Heterologous Gene Expression in Escherichia coli and Microalgae Anabaena

Construction of a Shuttle Vector for Heterologous Gene Expression in Escherichia coli and Microalgae Anabaena

Transplastomic plants for innovations in agriculture. A review

Molecular farming: production of drugs and vaccines in higher plants

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