Molecular detection and characterization of Anaplasma platys and Ehrlichia canis in dogs from the Caribbean

Alhassan, Andy; Hove, Paidashe; Sharma, Bhumika; Matthew-Belmar, Vanessa; Karasek, Inga; Lanza-Perea, Marta; Werners, Arno; Wilkerson, Melinda J.; Ganta, Roman R.

doi:10.1016/j.ttbdis.2021.101727

Cited by 21 publications

(16 citation statements)

References 81 publications

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“…and sand flies (e.g., Leishmania spp.) (Otranto 2018;Maggi and Krämer 2019;Alhassan et al 2021). The zoonotic potential of some CVBD-causing agents represents a threat to human health, especially in the tropics and subtropics, as the climatic conditions of these areas are conducive to the growth and proliferation of arthropod vectors (Dantas-Torres 2008;Maggi and Krämer 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Anaplasma platys , Ehrlichia canis and Babesia vogeli are the major causative agents of canine anaplasmosis, ehrlichiosis and babesiosis in Brazil (Dantas-Torres 2008 ), which are characterized by a variety of clinical signs (e.g., pyrexia, lethargy, weight loss, lymphadenopathy, bleeding tendencies, hemoglobinuria, petechiae, jaundice and even death) (Ybañez et al 2018 ) and hematological alterations (e.g., anemia and thrombocytopenia) in infected animals. The main biological vector of these hemoparasites is the brown dog tick (i.e., the recently proposed Rhipicephalus linneai , also referred to as the tropical lineage of Rhipicephalus sanguineus sensu lato by Šlapeta et al 2021 ), which has also been associated with the transmission of other pathogens, such as Hepatozoon canis and bacterial species belonging to the spotted fever group rickettsiae (SFGR) (Ramos et al 2014 ; Santos et al 2018 ; Alhassan et al 2021 ). Animals coinfected by multiple CVBD-causing pathogens are at higher risk of developing severe clinical signs (de Caprariis et al 2011 ).…”

Section: Introductionmentioning

confidence: 99%

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Vector-borne pathogens of zoonotic concern in dogs from a Quilombola community in northeastern Brazil

et al. 2022

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Canine vector-borne pathogens (CVBPs) comprise a group of disease agents mainly transmitted by ticks, fleas, mosquitoes and sand flies. In this study, we assessed the presence of CVBPs in an Afro-descendent community (Quilombola) of northeastern, Brazil. Dog blood samples (n = 201) were collected and analyzed by rapid test for the detection of antibodies against Leishmania spp., Anaplasma spp., Ehrlichia spp. and Borrelia burgdorferi sensu lato (s.l.), and antigens of Dirofilaria immitis. In addition, polymerase chain reactions were performed for Anaplasmataceae, Babesia spp., Hepatozoon spp., Rickettsia spp. and B. burgdorferi s.l. Overall, 66.7% of the dogs scored positive to at least one pathogen at serological and/or molecular methods. Antibodies against Ehrlichia spp. were the most frequently detected (57.2%; n = 115/201), followed by Anaplasma spp. (8.5%; n = 17/201), Leishmania spp. (8.5%; n = 17/201) and B. burgdorferi s.l. (0.5%; n = 1/201). For D. immitis, 11 out of 201 (5.5%) animals scored positive. At the molecular analysis, 10.4% (n = 21/201) of the samples scored positive for Babesia spp./Hepatozoon spp., followed by Anaplasmataceae (5.0%; n = 10/201) and Rickettsia spp. (3.0%; n = 6/201). All samples were negative for B. burgdorferi s.l. Our data demonstrated the presence of CVBPs in the studied population, with a high seropositivity for Ehrlichia spp. In addition, considering the detection of zoonotic pathogens in dogs and their relationship with people from Quilombola communities, effective control strategies are advocated for minimizing the risk of infection in this socially vulnerable human population and their pets.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Vector-borne pathogens of zoonotic concern in dogs from a Quilombola community in northeastern Brazil

et al. 2022

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“…The prevalence of infection with Ehrlichia spp. has been reported to vary in different parts of the world, as determined by various techniques (Ahmet et al 2001;Ansari-Mood et al 2015;Csokai et al 2017;Azhahianambi et al 2018;Bunroddith et al 2018;Piratae et al 2019;ALhassan et al 2021). In Indonesia, a study on Ehrlichia spp.…”

Section: Discussionmentioning

confidence: 99%

Clinical Observations, Hematological Profile, Serological Testing, and Molecular Detection of Ehrlichia canis in Veterinary Clinics in Bali, Indonesia

2022

Int J Vet Sci

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Ticks of Rhipicephalus species can serve as a vector for transmitting the Zoonotic Ehrlichiosis disease from dogs to humans. In Indonesia, epidemiological data on the prevalence of Ehrlichia canis are very limited. The incidence of Ehrlichiosis is usually reported based on clinical symptoms and serological results. In contrast, accurate diagnosis mainly relies on microscopic examination of a stained blood smear and polymerase chain reaction (PCR). The present study was conducted to evaluate the correlations among clinical observations, hematological profile, serological testing, and molecular detection of E. canis in dogs examined at different animal clinics in Bali, Indonesia. We collected 109 samples from Ehrlichiosis suspected dogs. The disease in these dogs was confirmed through a hematological profile, serologically, and PCR test. The hematological examination was performed with ICUBIO iCell-800Vet. We did a serological examination with Rapid Test BioNote© E. canis. DNA extraction was carried out with DNeasy Blood and Tissue Kit product Qiagen. PCR amplification was performed with Personal Thermal Cycler MJ Mini BIO-RAD. The results revealed that clinical signs such as epistaxis, fever, and pale mucous membranes were strongly associated with serological detection of E. canis. In contrast, none of these signs was significantly correlated with PCR detection of E. canis. Total erythrocytes counts were significantly associated with serologic detection. The total erythrocytes, thrombocytes, and hemoglobin levels were significantly associated with PCR detection. The 16S-rDNA, collected from PCR and amplified, showed the E. canis gene, indicating that E. canis organisms were found in Bali. E canis Bali is one cluster with clusters from South America, Europe, and Asian countries like Thailand, Taiwan, Japan, and Israel. In conclusion, E. canis infection was confirmed through clinical, serological, and molecular approaches in dogs in Bali, Indonesia.

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“…Anaplasma platys was first described in dogs from the USA by Harvey et al [73]. It has been reported to cause infectious canine cyclic-thrombocytopenia as well as infections in cats, cattle, goats, camels, and humans [74][75][76][77][78]. Recently, it has been reported that Rhipicephalus sanguineus s.l.…”

Section: Anaplasma/ehrlichia Speciesmentioning

confidence: 99%

Ticks and Tick-Borne Pathogens Abound in the Cattle Population of the Rabat-Sale Kenitra Region, Morocco

et al. 2021

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Tick-borne pathogens cause the majority of diseases in the cattle population in Morocco. In this study, ticks were collected from cattle in the Rabat-Sale-Kenitra region of Morocco and identified morphologically, while tick-borne pathogens were detected in cattle blood samples via polymerase chain reaction assay and sequencing. A total of 3394 adult ixodid ticks were collected from cattle and identified as eight different tick species representing two genera, Hyalomma and Rhipicephalus. The collected ticks consisted of Hyalomma marginatum, Hyalomma anatolicum excavatum, Rhipicephalus sanguineus sensu lato, Rhipicephalus bursa, Hyalomma detritum, Hyalomma lusitanicum, Hyalomma dromedarii, and Hyalomma impeltatum. The overall prevalence of tick-borne pathogens in blood samples was 63.8%, with 29.3% positive for Babesia/Theileria spp., 51.2% for Anaplasma/Ehrlichia spp., and none of the samples positive for Rickettsia spp. Sequencing results revealed the presence of Theileria annulata, Babesia bovis, Anaplasma marginale, Theileria buffeli, Theileria orientalis, Babesia occultans, Anaplasma phagocytophilum, Anaplasma capra, Anaplasma platys, Anaplasma bovis, Ehrlichia minasensis, and one isolate of an unknown bovine Anaplasma sp. Crossbreeds, females, older age, and high tick infestation were the most important risk factors for the abundance of tick-borne pathogens, which occurred most frequently in Jorf El Melha, Sidi Yahya Zaer, Ait Ichou, and Arbaoua locations.

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Molecular detection and characterization of Anaplasma platys and Ehrlichia canis in dogs from the Caribbean

Cited by 21 publications

References 81 publications

Vector-borne pathogens of zoonotic concern in dogs from a Quilombola community in northeastern Brazil

Vector-borne pathogens of zoonotic concern in dogs from a Quilombola community in northeastern Brazil

Clinical Observations, Hematological Profile, Serological Testing, and Molecular Detection of Ehrlichia canis in Veterinary Clinics in Bali, Indonesia

Ticks and Tick-Borne Pathogens Abound in the Cattle Population of the Rabat-Sale Kenitra Region, Morocco

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