Molecular detection and characterization of Anaplasma platys and Ehrlichia canis in dogs from northern Colombia

Pesapane, Risa; Foley, Janet E.; Thomas, Richard; Castro, Lyda R.

doi:10.1016/j.vetmic.2019.05.002

Cited by 21 publications

(18 citation statements)

References 29 publications

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“…The positivity to A. platys was significantly higher in females than in males, as opposed to previous studies from Egypt [ 14 , 31 ]. This may be related to higher tick exposure in females as compared to males in the area investigated in the present study.…”

contrasting

confidence: 99%

Molecular detection and risk factors for Anaplasma platys infection in dogs from Egypt

Selim

Al-Mohammed

Abdelhady³

et al. 2021

Parasites Vectors

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Background Anaplasma platys is a tick-borne bacterium which infects blood platelets of dogs, causing canine cyclic thrombocytopenia. The disease is distributed worldwide, particularly in the tropics and subtropics, but information on the epidemiology of A. platys infection in dogs is fragmentary in many countries, including Egypt. In this study, we investigated the prevalence and risk factors associated with A. platys infection in dogs from Egypt. Methods A conventional PCR targeting a fragment of the 16S rRNA gene of A. platys was used to screen 500 dogs from five North Egyptian governorates. DNA sequencing and phylogenetic analysis were performed for one of the positive samples. Results The overall prevalence of A. platys in the studied dogs was 6.4%. Females of the German shepherd breed without veterinary care had higher odds for A. platys positivity. High tick infestation and lack of anti-tick treatment were also identified as risk factors for A. platys infection. Phylogenetic analysis revealed that the sequence obtained herein was closely related to sequences from Egypt, South Africa and Uruguay. Conclusions This is the first large-scale epidemiological study of A. platys in Egypt, where female German shepherd dogs without veterinary care, as well as dogs with high tick infestation and without anti-tick treatment are at a higher risk of infection. Graphical abstract

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contrasting

confidence: 99%

Molecular detection and risk factors for Anaplasma platys infection in dogs from Egypt

Selim

Al-Mohammed

Abdelhady³

et al. 2021

Parasites Vectors

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“…In recent years, E. canis and its mixed infection with other pathogens have been reported in dogs in many countries and regions around the world [16][17][18][19]. Cicuttin et al [11] performed molecular detection of E. canis on the canine blood samples from Buenos Aires, Argentina, and the PCR-positive rate was 6.7% (15/223) .…”

Section: Discussionmentioning

confidence: 99%

Molecular detection and genetic variability of Ehrlichia canis in pet dogs in Xinjiang, China

et al. 2020

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Background and Aim: As a tick-borne zoonotic pathogen, Ehrlichia canis has already posed a threat to public health and safety. This study aimed to clarify the prevalence and molecular characteristics of E. canis in pet dogs in Xinjiang, China. Materials and Methods: A total of 297 blood samples of pet dogs and 709 skin ticks (Rhipicephalus sanguineus sensu lato) were subjected to molecular detection using PCR for E. canis 16S rRNA gene, and then, positive samples were amplified, sequenced, and phylogenetically analyzed for E. canis gp36 gene. Results: The PCR detection showed that the positive rate of PCR was 12.12% (36/297) in blood samples and 15.23% (108/709) in tick samples, respectively. Based on the phylogenetic analysis of E. canis gp36 protein, these E. canis strains in different geographical regions of the world can be divided into Genogroup I and Genogroup II. Among them, the Xinjiang epidemic strain XJ-6 and 533, 36, 1055, Kasur1, and Jake strains were clustered into subgroup 1.1 of Genogroup I, while the XJ-2, XJ-21, and XJ-35 strains and the TWN1, TWN4, CM180, and CM196 strains were closely related and belonged to subgroup 2.2 of Genogroup II, displaying high genetic diversity. Conclusion: This is the first study focusing on the molecular epidemiology of E. canis infection in pet dogs, which revealed that E. canis infection had been occurred in Xinjiang, China. More importantly, this study confirmed that the substantial variability in immunoreactive protein gp36 from E. canis strains circulating in pet dogs.

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“…This study was performed in Colombia, where there is also a lack of seroprevalence studies about Ehrlichia spp. (less than 20 in PubMed database), 11 in canine populations, none in Pereira and Risaralda [4,19,20,[46][47][48][49][50]. Given the high seroprevalence obtained in an area with ticks' infestation, the potential risk for zoonotic transmission to humans remains latent, deserving immediate seroprevalence studies.…”

Section: Discussionmentioning

confidence: 99%

“…Poverty is highly associated with these tick-borne pathogens in Pereira, as shown in the present study.Unfortunately, TBD, such as ehrlichiosis, anaplasmosis, and diro lariasis, are not reportable and currently not under surveillance in most Latin American countries [12][13][14][15][16][17]. In addition, there is a signi cant gap of knowledge pertaining their circulation across endemic areas in certain countries, such as Colombia [18][19][20][21][22]. This is why seroprevalence studies remain crucial to better understand the diverse epidemiological aspects of these vector-borne diseases.Herein, we present a prospective study aimed to evaluate the seroprevalence of Anaplasma phagocytophilum/platys‚ Borrelia burgdorferi sensu lato (s.l.…”

mentioning

confidence: 99%

Seroprevalence Canine Survey for Selected Vector-Borne Pathogens of and Its Relationship with Poverty in Metropolitan Pereira, Colombia, 2020

Bonilla-Aldana

Gutiérrez-Grajales

Martínez-Arboleda

et al. 2021

Preprint

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Background: Tick-borne diseases (TBD) and dirofilariasis are currently not under surveillance in most Latin American countries, and there is a significant lack of studies describing the current situation in most endemic areas, including Colombia. Seroprevalence studies are crucial for understanding the epidemiology of these vector-borne diseases.Methods: A serosurvey for TBD and dirofilariasis amongst 100 dogs was carried out in the municipality of Pereira, located in the Coffee-Triangle region, Colombia. Samples were tested using a rapid assay test system (SNAP® 4Dx®); based on an enzyme immunoassay technique‚ screening for antibodies to Anaplasma phagocytophilum/platys (sensitivity 99.1%)‚ Borrelia burgdorferi s.l. (98.8%), and Ehrlichia canis/ewingii (96.2%) by using specific antigens, and checking for Dirofilaria immitis antigen based on specific antibodies (99.2%). Bivariate analyses were performed on Stata®14, significant p<0.05. Results: Global seroprevalence to the selected vector-borne pathogens was 74% (95%CI 65-83%). The highest seroprevalence was found for Ehrlichia canis/ewingii (74%), followed by Anaplasma phagocytophilum/platys (16%). Seropositivity for Borrelia spp. and Dirofilaria spp. was 0%. All Anaplasma spp. seropositive dogs showed co-detection of Ehrlichia spp. (16%). Seroprevalence was significantly higher among dogs from families of lower socioeconomic status / level (I, 86%), followed by level II (74%), and III (36%) (p=0.001); no seropositive dogs were found in for levels IV and V (higher socioeconomic status). All dogs exhibiting anorexia (12%) were invariably seropositive (100%) (p=0.029). Seroprevalence was higher amongst those showing mucocutaneous paleness (95%) compared to those without paleness (68%) (p=0.013) (OR=9.3; 95%CI 1.18-72.9). There was high variability in seroprevalence through the studied areas, ranging from 0% (La Libertad Park) up to Combia, Cesar Nader, Las Brisas and Saturno localities (100%) (p=0.033). Conclusions: Given the high seroprevalence obtained in an area with documented presence of ticks, there is a potential risk for zoonotic transmission to humans. Further seroprevalence studies in humans are needed to assess the prevalence of infections. Poverty is highly associated with these tick-borne pathogens in Pereira, as shown in the present study.

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Molecular detection and characterization of Anaplasma platys and Ehrlichia canis in dogs from northern Colombia

Cited by 21 publications

References 29 publications

Molecular detection and risk factors for Anaplasma platys infection in dogs from Egypt

Molecular detection and risk factors for Anaplasma platys infection in dogs from Egypt

Molecular detection and genetic variability of Ehrlichia canis in pet dogs in Xinjiang, China

Seroprevalence Canine Survey for Selected Vector-Borne Pathogens of and Its Relationship with Poverty in Metropolitan Pereira, Colombia, 2020

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