Molecular Detection and Genetic Characteristics of Equine Herpesvirus in Korea

Seo, Min-Goo; Ouh, In-Ohk; Lee, Sang Kyu; Lee, Jong-Seok; Kwon, Oh-Deog; Kwak, Dongmi

doi:10.3390/pathogens9020110

Cited by 7 publications

(9 citation statements)

References 23 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, in the current study EHV-4 was not detected among apparently healthy equids. This finding was coincided with previous reports of Negussie et al (2017b) who reviewed that EHV-1 and EHV-4 were never detected among apparently healthyequids and EHVs infection was never detected in apparently healthy donkeysand Seo et al (2020) who elucidated that EHV-4 was not detected in any of the samples evaluated among apparently healthy horses. On the other hand, this re-4869 sult was in contrast to previous reports of Azab et al (2019) who documented that all EHVs were detected in apparently healthy equids.…”

Section: Discussionsupporting

confidence: 91%

Clinical and Molecular Epidemiological Study on Herpesviruses Infection among Equid Populations in Upper Egypt

Abdel-Rady

El-Rahim

El-Hameed³

et al. 2023

J Hellenic Vet Med Soc

View full text Add to dashboard Cite

The present study was carried out to record the clinical signs of equine herpesviruses (EHVs) infection and to detect the prevalence of EHVs infection among working equids in different provinces of Egypt. A total number of 115 working equids (92 horses and 23 donkeys) were clinically examined and sampled from November 2018 till November 2019 for this study. Two samples were collected from each animal (nasal swab and blood sample) and were subjected to multiplex-PCR to detect the prevalence of different EHVs infection among equids. In the current study, the overall prevalence of EHVs infection among equid populations in Egypt was 80% by using multiplex-PCR. Moreover, the most prevalent equine herpesvirus (EHV) among equids in Upper Egypt was EHV-2 (61.74%), followed by EHV-5 (43.48%), EHV-1 (20%), and EHV-4 (13.04%). The recorded clinical signs of the examined equids harbored EHVs (PCR-positive) can be summarized as follow: a higher percentage was detected among equids with a history of acute onset (59.78%), pyrexia (57.61%), and/or systemic illness (45.65%) with or without respiratory signs (56.52%) and ocular signs (35.87%). Furthermore, 4.35% and 1.09% of EHV-1 PCR-positive equids displayed neurological signs and abortion, respectively.

show abstract

Section: Discussionsupporting

confidence: 91%

Clinical and Molecular Epidemiological Study on Herpesviruses Infection among Equid Populations in Upper Egypt

Abdel-Rady

El-Rahim

El-Hameed³

et al. 2023

J Hellenic Vet Med Soc

View full text Add to dashboard Cite

show abstract

“…Although the ORF30 D752 genotypes (3/55, 5.5%) were reported in 2014 in Korea [ 18 ], this neurological genotype may not be common in Korea. Recently, similar studies were reported in other countries, and the prevalence of the neuropathogenic genotype was extremely low in Japan (2.7%), Australia (3%) and Argentina (7.4%) [ 23 ]. In particular, neuropathogenic cases were extremely rare in Korea [ 16 ].…”

Section: Discussionsupporting

confidence: 55%

“…Unfortunately, limited genetic data are available in Korea. To date, only ORF30 and ORF33 have been analyzed [ 19 , 20 , 23 ]; however, these sequences are not present in databases.…”

Section: Discussionmentioning

confidence: 99%

“…EHV-1 N752 genotype viruses are more commonly associated with abortion, responsible for 15~26% of the EHM outbreak [ 25 ]. All isolated viruses belonged to the ORF30 N752 genotype, indicating non-neuropathogenic EHV-1 in Korea [ 19 , 23 , 26 ]. Although the ORF30 D752 genotypes (3/55, 5.5%) were reported in 2014 in Korea [ 18 ], this neurological genotype may not be common in Korea.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of the Variability of the ORF34, ORF68, and MLST Genes in EHV-1 from South Korea

et al. 2021

View full text Add to dashboard Cite

Equine herpesvirus-1 (EHV-1) is an important pathogen in horses. It affects horses worldwide and causes substantial economic losses. In this study, for the first time, we characterized EHV-1 isolates from South Korea at the molecular level. We then aimed to determine the genetic divergences of these isolates by comparing them to sequences in databases. In total, 338 horse samples were collected, and 12 EHV-1 were isolated. We performed ORF30, ORF33, ORF68, and ORF34 genetic analysis and carried out multi-locus sequence typing (MLST) of 12 isolated EHV-1. All isolated viruses were confirmed as non-neuropathogenic type, showing N752 of ORF30 and highly conserved ORF33 (99.7–100%). Isolates were unclassified using ORF68 analysis because of a 118 bp deletion in nucleotide sequence 701–818. Seven EHV-1 isolates (16Q4, 19R166-1, 19R166-6, 19/10/15-2, 19/10/15-4, 19/10/18-2, 19/10/22-1) belonged to group 1, clade 10, based on ORF34 and MLST analysis. The remaining 5 EHV-1 isolates (15Q25-1, 15D59, 16Q5, 16Q40, 18D99) belonged to group 7, clade 6, based on ORF34 and MLST analysis.

show abstract

“…In a zoo, EHV‐1‐positive guinea pigs ( Cavia porcellus ) died after ataxia and paralytic disorders, showing also abortions and stillbirths (Wohlsein et al, 2011). Apparently accidental hosts such as Thomson’s gazelle ( Eudorcas thomson ) (Kennedy et al, 1996; Wohlsein et al, 2011; Guo et al, 2014; Sakaguchi et al, 2017), fallow deer ( Dama dama ) (Kinyili and Thorsen, 1979; Abdelgawad et al, 2016), blackbuck ( Antelopa cervicapra ) and giraffe ( Giraffa camelopardalis reticulate ) have been reported to be infected with EHV‐1 or EHV‐9 (Chowdhury et al, 1988; Borchers et al, 2006; Kasem et al, 2008). EHV‐9 can be also named Gazelle herpesvirus (GHV) (Fukushi et al, 1997).…”

Section: Assessmentmentioning

confidence: 99%

Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): infection with Equine Herpesvirus‐1

Nielsen¹,

Álvarez²,

Bicout³

et al. 2022

EFS2

View full text Add to dashboard Cite

Equine Herpesvirus‐1 infection has been assessed according to the criteria of the Animal Health Law (AHL), in particular criteria of: Article 7 on disease profile and impacts, Article 5 on the eligibility of the disease to be listed, Article 9 for the categorisation of the disease according to disease prevention and control measures as in Annex IV and Article 8 on the list of animal species related to Equine Herpesvirus‐1 infection. The assessment has been performed following a methodology composed of information collection and compilation, and expert judgement on each criterion at individual and collective level. The outcome is the median of the probability ranges provided by the experts, which indicates whether the criterion is fulfilled (66–100%) or not (0–33%), or whether there is uncertainty about fulfilment (33–66%). For the questions where no consensus was reached, the different supporting views are reported. According to the assessment performed, Equine Herpesvirus‐1 infection can be considered eligible to be listed for Union intervention according to Article 5 of the Animal Health Law with 33–90% certainty. According to the criteria as in Annex IV of the AHL related to Article 9 of the AHL for the categorisation of diseases according to the level of prevention and control, it was assessed with less than 1% certainty that EHV‐1 fulfils the criteria as in Section 1 (category A), 1–5% for the criteria as in Section 2 (category B), 10–66% for the criteria as in Section 3 (category C), 66–90% for the criteria as in Section 4 (category D) and 33–90% for the criteria as in Section 5 (category E). The animal species to be listed for EHV‐1 infection according to Article 8(3) criteria are the species belonging to the families of Equidae, Bovidae, Camelidae, Caviidae, Cervidae, Cricetidae, Felidae, Giraffidae, Leporidae, Muridae, Rhinocerontidae, Tapiridae and Ursidae.

show abstract

Molecular Detection and Genetic Characteristics of Equine Herpesvirus in Korea

Cited by 7 publications

References 23 publications

Clinical and Molecular Epidemiological Study on Herpesviruses Infection among Equid Populations in Upper Egypt

Clinical and Molecular Epidemiological Study on Herpesviruses Infection among Equid Populations in Upper Egypt

Evaluation of the Variability of the ORF34, ORF68, and MLST Genes in EHV-1 from South Korea

Assessment of listing and categorisation of animal diseases within the framework of the Animal Health Law (Regulation (EU) No 2016/429): infection with Equine Herpesvirus‐1

Contact Info

Product

Resources

About