Molecular detection and genotyping of human papillomavirus in cervical carcinoma biopsies in an area of high incidence of cancer from Moroccan women

Khair, M.; Mzibri, Mohammed El; Mhand, Rajaa Aït; Benider, Abdellatif; Benchekroun, N.; Fahime, Elmostafa El; Benchekroun, Mohamed Nabil; Ennaji, Moulay Mustapha

doi:10.1002/jmv.21279

Cited by 29 publications

(35 citation statements)

References 40 publications

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“…The second prevalent HPV type detected in our NPC biopsies is HPV59 (16,7%). Of interest, HPV-16 and -18, which are the most virulent genotypes associated with CC in Moroccan woman (35% to 45%) [21,22], were detected in very few Moroccan NPC cases (8.3%), and similar data were reported in an Iranian study [23]. …”

Section: Discussionsupporting

confidence: 64%

Human papillomavirus detection in moroccan patients with nasopharyngeal carcinoma

Laantri

Attaleb

Kandil

et al. 2011

Infect Agents Cancer

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BackgroundNasopharyngeal carcinoma (NPC) is a malignant tumor which arises in surface epithelium of the posterior wall of the nasopharynx. There's is evidence that Epstein Barr virus (EBV) is associated to NPC development. However, many epidemiologic studies point to a connection between viral infections by the human papillomavirus (HPV) and NPC.MethodSeventy Moroccan patients with NPC were screened for EBV and HPV. EBV detection was performed by PCR amplification of BZLF1 gene, encoding the ZEBRA (Z Epstein-Barr Virus Replication Activator) protein, and HPV infection was screened by PCR amplification with subsequent typing by hybridization with specific oligonucleotides for HPV types 16, 18, 31, 33, 35, 45 and 59.ResultsThe age distribution of our patients revealed a bimodal pattern. Sixty two cases (88.9%) were classified as type 3 (undifferentiated carcinoma), 6 (8.6%) as type 2 (non keratinizing NPC) and only 2 (2.9%) cases were classified as type 1 (keratinizing NPC). EBV was detected in all NPC tumors, whereas HPV DNA was revealed in 34% of cases (24/70). Molecular analysis showed that 20.8% (5/24) were infected with HPV31, and the remaining were infected with other oncogenic types (i.e., HPV59, 16, 18, 33, 35 and 45). In addition, statistical analysis showed that there's no association between sex or age and HPV infection (P > 0.1).ConclusionOur data indicated that EBV is commonly associated with NPC in Moroccan patients and show for the first time that NPC tumours from Moroccan patients harbour high risk HPV genotypes.

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Section: Discussionsupporting

confidence: 64%

Human papillomavirus detection in moroccan patients with nasopharyngeal carcinoma

Laantri

Attaleb

Kandil

et al. 2011

Infect Agents Cancer

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“…The second prevalent HPV type detected in Moroccan NPC biopsies is HPV59 (16,7%). Of interest, HPV-16 and -18, which are the most virulent genotypes associated with CC in Moroccan woman (35% to 45 %) (Khair et al 2009), were detected in very few NPC cases (8.3 %), and similar data were reported in an Iranian study (Mirzamani et al 2006). However, a recent study suggests that WHO-I NPC may be associated with oncogenic HPV.…”

Section: Wwwintechopencomsupporting

confidence: 73%

Role of the Epstein-Barr Virus ZEBRA Protein and HPV in the Carcinogenesis of Nasopharyngeal Carcinoma

Moumad¹,

Laantri²,

Mohammed³

et al. 2012

Carcinogenesis, Diagnosis, and Molecular Targeted Treatment for Nasopharyngeal Carcinoma

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“…This result seems to be in line with previous reports [36,44]. In contrast, this molecular epidemiology profile was not consistent with observed in HIV-negative women [6][7][8] or women with CC in Morocco [9][10][11][12], in whom HPV 16 and 18 predominate. Such discrepancies between groups (HIVpositive and HIV-negative) has been reported in previous studies and may partly due to immune system deficiency [30,45].…”

Section: Discussioncontrasting

confidence: 39%

“…Previous studies in Moroccan women have been reported an inconsistent prevalence of HPV with 15.7% [6] in Rabat and 42.5-43.1% in Fez region [7,8]. However, the prevalence was much higher, ranging from 62% to 92%, among Moroccan women with CC [9][10][11][12]. The joint United Nations Programme on HIV/AIDS (UNAIDS) estimated that 30,000 (22,000 -40,000) Moroccans were living with HIV/AIDS at 2012, and women aged 15 years old and older living with HIV was 11,000 (8,500 -14,000).…”

Section: Introductionmentioning

confidence: 62%

The Human papillomavirus among women living with Human Immunodeficiency Virus in Morocco A prospective cross-sectional study

Ouladlahsen¹,

Fayssel

Bensghir³

et al. 2018

J Infect Dev Ctries

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Introduction: Women infected with human immunodeficiency virus (HIV) have a higher risk of contracting human papillomavirus (HPV) infections and are more prone to develop cervical cancer. The objective of this study was to determine the prevalence of HPV and its association with risk factors among Moroccan women living with HIV/AIDS. Methodology: We enrolled 251 HIV-infected non-pregnant women in Morocco from February 2013 to September 2016. Sociodemographic, lifestyles, behavioral and clinical data were collected. Polymerase chain reaction followed by sequencing were performed for molecular detection and HPV genotyping in cervical samples, respectively. Results: Abnormal cervical smears were found in 34/246 patients (13.82%). The overall prevalence of HPV was 74.50%. HPV 58 was the most prevalent (39.29%) followed by HPV 18 (10.71%), HPV 70 (8.93%), HPV 33 (7.14%), HPV 6 (6.25%) and other genotypes (< 3%). Overall, high-risk HPV (HR-HPV) types were present in 75% (84/112) of patients and the prevalence of HR-HPV types in samples with abnormal Pap was higher than in normal Pap (55/83, 66.27% vs. 28/83,33.33%, p < 0.0001). Univariate analyses showed that none of the socio-demographic and behaviors factors was associated with HPV infection. Moreover, Pap results were not affected by HPV status (p = 0.532). Whereas, CD4 T-cell counts above 200/mm 3 at enrolment were apparently not protective to HPV infection. We found a high prevalence of HPV infection and HR-HPV types among HIV-positive women that significantly associated with abnormal Pap. Conclusion: Our findings suggest a high prevalence of HPV infection with high-risk types was observed among HIV-positive women warrant to implement a regular screening by Pap smear.

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Molecular detection and genotyping of human papillomavirus in cervical carcinoma biopsies in an area of high incidence of cancer from Moroccan women

Cited by 29 publications

References 40 publications

Human papillomavirus detection in moroccan patients with nasopharyngeal carcinoma

Human papillomavirus detection in moroccan patients with nasopharyngeal carcinoma

Role of the Epstein-Barr Virus ZEBRA Protein and HPV in the Carcinogenesis of Nasopharyngeal Carcinoma

The Human papillomavirus among women living with Human Immunodeficiency Virus in Morocco A prospective cross-sectional study

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