Molecular Detection and Genotyping of <i>Toxoplasma gondii</i> from Pigs for Human Consumption in Zhejiang and Jiangsu Provinces, Eastern China

Sun, Hongchao; Fu, Yuan; Yuan, Xiufang; Li, Junxing; Xu, Li-Hua; Zhang, Jianing; Yu, Bin; Huang, Jing; Qi, Meng; Shi, Tuanyuan

doi:10.1089/fpd.2022.0036

Cited by 1 publication

(1 citation statement)

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“…Significant differences in prevalence were found among the different regions. This may have been because of the choice of detection method employed during the epidemiological investigation; the geographical location itself; seasonal factors such as precipitation, temperature, and air pressure; specific farm feeding modes; and other factors [26]. Notably, free-range chickens are at higher risk of T. gondii infection due to their proximity to soil, plants, or water sources contaminated by T. gondii oocysts.…”

Section: Discussionmentioning

confidence: 99%

First Molecular Detection and Genotype Identification of Toxoplasma gondii in Chickens from Farmers’ Markets in Fujian Province, Southeastern China

Chu,

Huang,

Miao

et al. 2023

Pathogens

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Toxoplasma gondii is an opportunistic pathogenic protozoan that can infect all nucleated cells in almost all warm-blooded animals, including humans. T. gondii infection has been reported in many food animals worldwide. However, the prevalence and genotypes of T. gondii in chickens from farmers’ markets in Fujian province in southeastern China remain unreported. In the present study, four tissue samples from each of the 577 chickens (namely, the heart, liver, lungs, and muscles) were collected from farmers’ markets in five regions of Fujian province (Zhangzhou, Sanming, Quanzhou, Fuzhou, and Longyan). We first analyzed the prevalence and genotypes of T. gondii using PCR targeting of the B1 gene of T. gondii. Of the 577 chickens, thirty-two (5.5%) tested positive for the B1 gene. Among the five regions, Sanming had the highest infection rate (16.8%, 16/95), followed by Quanzhou (8.0%, 8/100), Longyan (5.0%, 5/100), Zhangzhou (1.1%, 2/182), and Fuzhou (1.0%, 1/100). Among these thirty-two T. gondii-positive chickens, the infection rates of the lungs, heart, liver, and muscles were 68.8% (22/32), 34.4% (11/32), 28.1% (11/32), and 9.4% (3/32), respectively. Significant differences in prevalence were found among the different regions (χ2 = 35.164, p < 0.05) and tissues (χ2 = 25.874, p < 0.05). A total of 128 tissue and organ samples of the thirty-two T. gondii-positive chickens from the different regions were analyzed using PCR–restriction fragment length polymorphism (PCR–RFLP) on the basis of 10 genetic markers. Seven tissue samples (lung samples from five chickens, heart samples from one chicken, and liver samples from one chicken) underwent successful amplification at all the genetic markers, and all the T. gondii genotypes were identified as genotype I (ToxoDB #10). These findings serve as a foundation for evaluating the risk of T. gondii contamination in chicken products intended for human consumption and offer insight into preventing the transmission of the parasite from chickens to humans.

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Section: Discussionmentioning

confidence: 99%