Molecular detection and identification of Rickettsiales pathogens in dog ticks from Costa Rica

Campos-Calderón, Liliana; Ábrego-Sánchez, Leyda; Morales, Álvaro; Alberti, Alberto; Tore, Gessica; Zobba, Rosanna; Jiménez-Rocha, Ana E.; Dolz, Gaby

doi:10.1016/j.ttbdis.2016.07.015

Cited by 33 publications

(30 citation statements)

References 27 publications

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“…may be detected by the used commercial rapid ELISA. However, we did not test for the presence of E. ewingii or E. chaffeensis by PCR here, as these pathogens have not been detected in ticks or domestic animals in Central America so far [ 10 , 34 , 35 ]. However, E. chaffeensis DNA was isolated from human patients in neighboring Costa Rica [ 36 ].…”

Section: Discussionmentioning

confidence: 99%

“…As mentioned above, ixodid ticks, which are known to transmit A. phagocytophilum , are rather rare in Central America. Previously, only A. platys was reported to infect dogs in Nicaragua [ 15 ] and Costa Rica [ 10 ]; however, A. phagocytophilum has been found at low prevalence in Rhipicephalus ticks collected from dogs in Costa Rica [ 35 ]. It remains to be proven whether or not ticks other than Ixodes spp.…”

Section: Discussionmentioning

confidence: 99%

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Seroprevalence and current infections of canine vector-borne diseases in Nicaragua

et al. 2018

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BackgroundVector-borne diseases constitute a major problem for veterinary and public health, especially in tropical regions like Central America. Domestic dogs may be infected with several vector-borne pathogens of zoonotic relevance, which may also severely compromise canine health.MethodsTo assess the prevalence of canine vector-borne diseases in Nicaragua, 329 dogs from seven cities, which were presented to the veterinarian for various reasons, were included in this study. Dogs were examined clinically and diagnostic blood samples were taken for analysis of packed cell volume (PCV) and presence of microfilariae as well as antigen of Dirofilaria immitis and antibodies to Ehrlichia spp., Anaplasma spp. and Borrelia burgdorferi (sensu lato) by use of a commercially available rapid ELISA. To detect current infections, specific PCRs for the detection of E. canis, A. platys and A. phagocytophilum were carried out on blood samples of the respective seropositive dogs. Microfilaremic blood samples, as well as D. immitis antigen positive samples were further subjected to PCR and subsequent sequencing for filarial species identification.ResultsAntibodies against Ehrlichia spp. were present in 62.9% of dogs, while Anaplasma spp. seroprevalence was 28.6%. Antibodies against species of both genera were detected in 24.9% of dogs. Borrelia burgdorferi (s.l.) antibodies were not detected. Dirofilaria immitis antigen was present in six animals (1.8%), two of which also showed D. immitis microfilariae in buffy coat. In addition to D. immitis, Acanthocheilonema reconditum was identified by PCR and sequencing in two of four additional microfilaremic blood samples, which were tested negative for D. immitis antigen. Current E. canis infections as defined by DNA detection were present in 58.5% of Ehrlichia-seropositive dogs, while 5.3% of Anaplasma-seropositive dogs were PCR-positive for A. platys, 2.2% for A. phagocytophilum and 16.0% for both Anaplasma species. Current E. canis infection had a statistically significant negative impact on PCV, whereas no relationship between infection status and clinical signs of disease could be observed.ConclusionsThese results indicate that canine vector-borne diseases are widespread in Nicaragua and that dogs may constitute a reservoir for human infection with E. canis, A. phagocytophilum and D. immitis. Thus, the use of repellents or acaricides to protect dogs from vector-borne diseases is strongly recommended.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Seroprevalence and current infections of canine vector-borne diseases in Nicaragua

et al. 2018

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“…Although DNA presence of A. phagocytophilum in dogs was reported previously in two dogs, one white tailed deer (Dolz et al, 2013) and two Rhipicephalus sanguineus s.l. ticks (Campos-Calderón et al, 2016), a nationwide representative investigation of A. phagocytophilum was not carried out before.…”

Section: Discussionmentioning

confidence: 99%

“…Recently, 16S rRNA/groEL amplification and sequence analyses provided evidence for Anaplasma platys, Anaplasma phagocytophilum, and A. platys-like infection in Rhipicephalus sanguineus s.l. ticks (Campos-Calderón et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Characterization of Anaplasma spp. infection in dogs from Costa Rica

Bonilla

Campos-Calderón

Jiménez-Rocha

et al. 2017

Veterinary Parasitology: Regional Studies and Reports

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A cross-sectional study combining serological and molecular techniques for detecting selected Anaplasma species was conducted between 2011 and 2012 in dogs and ticks sampled in all provinces of Costa Rica. Global Anaplasma spp. seroprevalence was 2.7% (11/408) by indirect immunofluorescence assay. The 16S rRNA PCR confirmed active A. phagocytophilum infection only in one dog (0.3%, 1/374); however, the same sample was negative to groEL PCR. Out of 122 Rhipicephalus sanguineus s.l. ticks analyzed, one (0.8%) was found positive to A. phagocytophilum 16S rRNA PCR but negative when tested by groEL PCR; this tick was collected from a seronegative and PCR negative dog. Both 16S rRNA sequences were 100% (510/510 bp) identical to A. phagocytophilum strains isolated in different countries from different hosts. The presence of A. platys was established in four dogs (1%, 4/374) by both 16SrRNA and groEL PCR. Ticks collected from the same dogs tested negative by PCR. The 16S rRNA sequences were 100% identical to the corresponding sequences of A. platys strains isolated from dogs in Croatia and Brazil, however groEL sequences showed variable similarity levels (99-100%) with different strains of A. platys isolated in Chile, Japan and Thailand, pointing out the possible presence of different variants in Central America. Collectively data indicate low prevalence of A. phagocytophilum and A. platys in dogs from Costa Rica. Furthermore, infections seem to occur without clinical signs but with some hematological changes, and seem to resolve without treatment.

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“…También, han sido usados para la exploración de las fuentes alimenticias de garrapatas, donde se requiere la amplificación de regiones mitocondriales de vertebrados, a partir de la sangre ingerida por el ectoparásito (Pichon et al 2003;Pichon et al 2005). De la misma forma, para la detección de agentes patógenos transmitidos por garrapatas, como rickettsiales, borrelias y piroplasmas (Guedes et al 2005; Barbieri et al 2013;Nava et al 2014;Campos-Calderón et al 2016).…”

Section: Introductionunclassified

Comparación de tres métodos de extracción de ADN a partir de garrapatas duras (Acari: Ixodidae)

Guevara-Vega

Morinson

Paternina

2019

Rev. U.D.C.A Act. & Div. Cient.

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Molecular detection and identification of Rickettsiales pathogens in dog ticks from Costa Rica

Cited by 33 publications

References 27 publications

Seroprevalence and current infections of canine vector-borne diseases in Nicaragua

Seroprevalence and current infections of canine vector-borne diseases in Nicaragua

Characterization of Anaplasma spp. infection in dogs from Costa Rica

Comparación de tres métodos de extracción de ADN a partir de garrapatas duras (Acari: Ixodidae)

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