Molecular detection and identification of Rickettsia felis in Polygenis (Siphonaptera, Rhopalopsyllidae, Rhopalopsyllinae) associated with cricetid rodents in a rural area from central Argentina

Melis, Mauricio; Espinoza-Carniglia, Mario; Savchenko, Ekaterina; Nava, Stefano; Lareschi, Marcela

doi:10.1016/j.vprsr.2020.100445

Cited by 3 publications

(4 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Those fleas that need to be prepared and identified using the optic microscope after the extraction of DNA were individually analysed by a non-destructive method of DNA extraction. This method consists of making an abdominal incision in each flea, using a sterile scalpel, to preserve the exoskeleton for further preparation of identification by optic microscopy (Lawrence et al, 2014;Miura et al, 2017;Melis et al, 2020).…”

Section: Rickettsia Survey: Dna Extraction Amplification and Sequencingmentioning

confidence: 99%

“…Regarding fleas, there is very little information about rickettsiae species associated with these arthropods in Argentina; R. felis has been found in C. f . felis collected on peridomestic dogs in Santa Fe Province (Nava et al, 2008), and recently Melis et al (2020) detected R. felis in Polygenis (Polygenis) axius axius (Rhopalopsyllidae) collected from a synanthropic cricetid rodent in Buenos Aires Province.…”

Section: Introductionmentioning

confidence: 99%

“…130 species and subspecies belonging to 11 families (Lareschi et al, 2016). Currently, four human-pathogenic species of Rickettsia are found in the country: Rickettsia rickettsii, Rickettsia parkeri and Rickettsia massiliae in ticks naturally infected (Cicuttin & Nava, 2013;Lamattina et al, 2018), and R. felis that infects fleas (Nava et al, 2008;Melis et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Ecology of fleas and their hosts in the trifinio of north‐east Argentina: first detection of Rickettsia asembonensis in Ctenocephalides felis felis in Argentina

Urdapilleta

Pech-May

Lamattina

et al. 2021

Medical Vet Entomology

View full text Add to dashboard Cite

Fleas are important in public health due to their role as parasites and vectors of pathogens, including Rickettsia. The aim of this study was to evaluate the diversity, abundance and prevalence of fleas and the presence of Rickettsia in the trifinio of north-east Argentina. Fleas from household and synanthropic animals were obtained from urban and periurban areas. They were taxonomically identified and samples of 227 fleas in 86 pools were analysed by polymerase chain reaction targeting the gltA and ompB genes of Rickettsia spp. The study revealed that Ctenocephalides felis felis was dominant on dogs, cats and opossums, with higher prevalence in the periurban area. The Shannon-Wiener and Morisita-Horn indices expressed differences in the diversity and similarity values of the absolute abundances of the species between the areas compared. DNA amplifications revealed 30.8% C. f . felis pools positive for Rickettsia spp. Phylogenetic analysis showed that the haplotype obtained was identical to Rickettsia asembonensis from Peru and Brazil. This is the first detection in Argentina of R. asembonensis that infects C. f . felis, and we emphasize the importance of conducting research from a 'One Health' perspective on the role of opossums and rodents in the integration of the transmission cycles of rickettsial bacteria.

show abstract

Section: Rickettsia Survey: Dna Extraction Amplification and Sequencingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Ecology of fleas and their hosts in the trifinio of north‐east Argentina: first detection of Rickettsia asembonensis in Ctenocephalides felis felis in Argentina

Urdapilleta

Pech-May

Lamattina

et al. 2021

Medical Vet Entomology

View full text Add to dashboard Cite

show abstract

“…So, species of this genus are being considered highly efficient vector for this disease, proving to be even more efficient than the main vector -Xenopsylla cheopis (Holdenried, 1952;Macchiavello, 1957;. Another pathogen that is recently associated with this genus is Rickettsia felis, initially detected in Ctenocephalides felis felis, Melis et al (2020) suggesting that this flea is capable of maintaining and transmitting Rickettsia in forest environments. Besides that, Schott et al (2020) identified Bartonella sp.…”

Section: Family Rhopalopsyllidae Oudemans 1909mentioning

confidence: 99%

>b<Ectoparasitofauna (Acari: Ixodida and Gamasida; Insecta:Siphonaptera) from terrestrial mammals>/b<: diversity, taxonomic aspects and associated zoonotic rickettsiales in Legado das Águas - Reserva Votorantim, in Tapiraí and Miracatu, São Paulo

Pesenato

View full text Add to dashboard Cite

who gave me the opportunity to learn immensely as a professional and as a person, both in the laboratory and in the field, and for having believed in my potential even before they met me. To MSc. Luís Flávio Costa, MSc.Renato Alvisi and Dr. Ana Rita Nardi for believing that I had the academic profile and for encouraging me to pursue this area since I was in college.To my field trip friends, Herbert Soares, Jaciara Costa, Thiago Fakelmann, Giovanna Nosberto and Mayara Bispo, for always being there to share laughs and sweat during the days and nights of collecting. For all the Legado das Águas team who accompanied our group on the crazy field trips, having fun with us, especially Miguel and José for their help with trails and traps. To the members of the Ribeirão da Anta Community who opened their homes and hearts to welcome us and taught us so much with their years of experience in the forest, especiallyValter for accompanying us on the trails and Val with her delicacies from the gods.To my lab friends who, as well as teaching me so many techniques and tips, also supported me in the endless samples and tests I needed to carry out. To Ryan Silva and Jaciara Costa for welcoming me so well into the 'Tryp/Leish' group and accompanying me during my trial period, to Carolina Serpa for her endless experience with serology, molecular and cell culture and to my 'Ticks' friends for the breakfast conversations and discussions of articles:

show abstract

Fleas (Siphonaptera, Latreille, 1825) from Rio Grande do Sul State, Brazil: Species Diversity, Hosts, and One Health Approach

Schott,

Ribeiro,

Santos

et al. 2024

Vector-Borne and Zoonotic Diseases

View full text Add to dashboard Cite

Molecular detection and identification of Rickettsia felis in Polygenis (Siphonaptera, Rhopalopsyllidae, Rhopalopsyllinae) associated with cricetid rodents in a rural area from central Argentina

Cited by 3 publications

References 16 publications

Ecology of fleas and their hosts in the trifinio of north‐east Argentina: first detection of Rickettsia asembonensis in Ctenocephalides felis felis in Argentina

Ecology of fleas and their hosts in the trifinio of north‐east Argentina: first detection of Rickettsia asembonensis in Ctenocephalides felis felis in Argentina

>b<Ectoparasitofauna (Acari: Ixodida and Gamasida; Insecta:Siphonaptera) from terrestrial mammals>/b<: diversity, taxonomic aspects and associated zoonotic rickettsiales in Legado das Águas - Reserva Votorantim, in Tapiraí and Miracatu, São Paulo

Fleas (Siphonaptera, Latreille, 1825) from Rio Grande do Sul State, Brazil: Species Diversity, Hosts, and One Health Approach

Contact Info

Product

Resources

About