Molecular detection and immunological localization of gill Na+/H+exchanger in the dogfish (Squalus acanthias)

Claiborne, James B.; Choe, Keith P.; Morrison-Shetlar, Alison I.; Weakley, Jill C.; Havird, Justin C.; Freiji, Abe; Evans, David H.; Edwards, Scott A.

doi:10.1152/ajpregu.00718.2007

Cited by 22 publications

(11 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The molecular identity and cellular localization of NHE isoforms and H ϩ -ATPase were well reported in the marine dogfish (Squalus acanthias) and longhorn sculpin (Myoxocephalus octodecemspinosus) and euryhaline stingray (Dasyatis sabina), and two distinct ionocytes, one expressing apical NHE3/2 and the other basolateral HA, were consistently identified and proposed as conducting acid and base secretion, respectively (6,8,9,10,33,40,41). Similar to those species, medaka, a euryhaline teleost, also has two distinct ionocytes, apical NHE3-and basolateral H ϩ -ATPase-expressing MR cells, in FW environments.…”

Section: Discussionmentioning

confidence: 74%

Acid secretion by mitochondrion-rich cells of medaka (Oryzias latipes) acclimated to acidic freshwater

Lin

Hsu

et al. 2012

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

In the present study, medaka embryos were exposed to acidified freshwater (pH 5) to investigate the mechanism of acid secretion by mitochondrion-rich (MR) cells in embryonic skin. With double or triple in situ hybridization/immunocytochemistry, the Na(+)/H(+) exchanger 3 (NHE3) and H(+)-ATPase were localized in two distinct subtypes of MR cells. NHE3 was expressed in apical membranes of a major proportion of MR cells, whereas H(+)-ATPase was expressed in basolateral membranes of a much smaller proportion of MR cells. Gill mRNA levels of NHE3 and H(+)-ATPase and the two subtypes of MR cells in yolk sac skin were increased by acid acclimation; however, the mRNA level of NHE3 was remarkably higher than that of H(+)-ATPase. A scanning ion-selective electrode technique was used to measure H(+), Na(+), and NH(4)(+) transport by individual MR cells in larval skin. Results showed that Na(+) uptake and NH(4)(+) excretion by MR cells increased after acid acclimation. These findings suggested that the NHE3/Rh glycoprotein-mediated Na(+) uptake/NH(4)(+) excretion mechanism plays a critical role in acidic equivalent (H(+)/NH(4)(+)) excretion by MR cells of the freshwater medaka.

show abstract

Section: Discussionmentioning

confidence: 74%

Acid secretion by mitochondrion-rich cells of medaka (Oryzias latipes) acclimated to acidic freshwater

Lin

Hsu

et al. 2012

American Journal of Physiology-Regulatory, Integrative and Comp

View full text Add to dashboard Cite

show abstract

“…Sections were labeled with DAPI-containing mounting media to show cell nuclei (blue). that are believed to be functionally analogous to the base secreting (B-type) intercalated cells of the mammalian collecting duct (Perry et al, 2003a;Perry et al, 2003b;Evans et al, 2005;Perry and Gilmour, 2006;Marshall and Grosell, 2006;Tresguerres et al, 2006;Claiborne et al, 2008). Another subset of ionocytes is thought to be analogous to the acid secreting (A-type) intercalated cells of the collecting duct in which an apical membrane Na + /H + exchanger and/or Na + channel/VATPase linked process acts to absorb Na + (Reid et al, 2003;Lin et al, 2006;Horng et al, 2007;Parks et al, 2007;Yan et al, 2007;Hwang and Lee, 2007).…”

Section: The Distribution and Putative Functions Of Ionocytes In Fishmentioning

confidence: 99%

The effects of thermally induced gill remodeling on ionocyte distribution and branchial chloride fluxes in goldfish (Carassius auratus)

Mitrovic

Perry

2009

Journal of Experimental Biology

View full text Add to dashboard Cite

SUMMARYExperiments were performed to evaluate the effects of temperature-induced changes in functional gill lamellar surface area on the distribution of ionocytes and branchial chloride fluxes in goldfish (Carassius auratus). In fish acclimated to warm water (25°C), the ionocytes were scattered along the lamellae and within the interlamellar regions of the filament. In cold water (7°C), the ionocytes were largely absent from the lamellae and filaments but instead were mostly confined to the outer regions of an interlamellar cell mass (ILCM) that formed within the interlamellar channels. Using a 'time-differential double fluorescent staining' technique, it was determined that in fish transferred from 25° to 7°C, the ionocytes on the outer edge of (and within) the ILCM originated predominantly from the migration of pre-existing ionocytes and to a lesser extent from the differentiation of progenitor cells. Despite the greater functional lamellar surface area in the warm-water-acclimated fish, there was no associated statistically significant increase in passive branchial Cl -efflux. Because the paracellular efflux of polyethylene glycol was increased 2.5-fold at the warmer temperature, it would suggest that goldfish specifically regulate (minimize) Cl -loss that otherwise would accompany the increasing functional lamellar surface area. In contrast to predictions, the numbers and sizes of individual ionocytes was inversely related to functional lamellar surface area resulting in a markedly greater ionocyte surface area in fish acclimated to cold water (5219±438 compared with 2103±180 μm 2 mm -1 of filament). Paradoxically, the activity of Na ) despite the greater numbers of ionocytes. There were no statistically significant differences in the rates of Cl -uptake in the two groups of fish despite the differences in ionocyte abundance. It is possible that to maintain normal rates of Cl -uptake, a greater ionocyte surface area is required in the cold-water fish that possess an ILCM because of the unfavorable positioning of the ionocytes on and within the ILCM, a structure lacking any obvious blood supply.

show abstract

“…Based on thermodynamic constraints (Avella and Bornancin, 1989), it was thought that electroneutral Na + /H + exchange, while likely in seawater (SW) fish, would be unlikely to contribute to Na + uptake in freshwater (FW) species (Perry, 1997;Marshall, 2002). However, the results of more recent studies have clearly demonstrated that Na + /H + exchangers (NHEs) are expressed in the gills of both SWand FW-acclimated fish, thus indirectly suggesting their involvement in Na + uptake in both media (Claiborne et al, 1999;Claiborne et al, 2008;Edwards et al, 2001;Edwards et al, 2002;Choe et al, 2002;Choe et al, 2005;Choe et al, 2007;Hirata et al, 2003;Scott et al, 2005;Tresguerres et al, 2005;Tresguerres et al, 2006b;Catches et al, 2006). Specifically, two paralogs within the SLC9 (NHE) gene family (reviewed by Orlowski and Grinstein, 2004) have been implicated as likely candidates for apical Na + /H + exchange: SLC9A2 (NHE2) and SLC9A3 (NHE3).…”

Section: Introductionmentioning

confidence: 99%

Branchial expression and localization of SLC9A2 and SLC9A3 sodium/hydrogen exchangers and their possible role in acid–base regulation in freshwater rainbow trout (Oncorhynchus mykiss)

Ivanis

Esbaugh

Perry

2008

Journal of Experimental Biology

118

View full text Add to dashboard Cite

SUMMARY-uptake and base excretion. Levels of mRNA for NHE2, the predominant isoform in the gill, were increased during 72 h of hypercapnic acidosis; NHE3 mRNA and protein levels were unaffected. Because plasma cortisol levels were increased during hypercapnia (from 35.3±9.4 to 100.1±30.9 ng ml -1 ), the effects of experimentally elevated cortisol levels on NHE expression were investigated. The elevation of plasma cortisol using intraperitoneal implants caused a significant increase in NHE2 mRNA expression without affecting NHE3 mRNA or protein abundance. Thus, we suggest that NHE2 contributes to acid-base regulation during hypercapnia owing to its transcriptional regulation by cortisol. The finding of NHE expression in PNA + MRCs is discussed with reference to current models of ionic and acid-base regulation in teleost fish.

show abstract

Molecular detection and immunological localization of gill Na⁺/H⁺exchanger in the dogfish (Squalus acanthias)

Cited by 22 publications

References 62 publications

Acid secretion by mitochondrion-rich cells of medaka (Oryzias latipes) acclimated to acidic freshwater

Acid secretion by mitochondrion-rich cells of medaka (Oryzias latipes) acclimated to acidic freshwater

The effects of thermally induced gill remodeling on ionocyte distribution and branchial chloride fluxes in goldfish (Carassius auratus)

Branchial expression and localization of SLC9A2 and SLC9A3 sodium/hydrogen exchangers and their possible role in acid–base regulation in freshwater rainbow trout (Oncorhynchus mykiss)

Contact Info

Product

Resources

About