Molecular Detection and Phylogenetic Analysis of Anaplasma phagocytophilum in Horses in Korea

Seo, Min-Goo; Ouh, In-Ohk; Choi, Eunsang; Kwon, Oh-Deog; Kwak, Dongmi

doi:10.3347/kjp.2018.56.6.559

Cited by 12 publications

(11 citation statements)

References 27 publications

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“…The high homology of A. phagocytophilum from different areas (100%) demonstrates the low variation of A. phagocytophilum distributed throughout the ROK. In addition, the A. phagocytophilum sequences detected in ticks during this study demonstrated 100% similarity to those previously detected in infected horses [ 37 ]. Various gene fragments have been used for identification of A. phagocytophilum [ 38 ].…”

Section: Discussionsupporting

confidence: 73%

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Molecular Detection and Phylogenetic Analysis of Tick-Borne Pathogens in Ticks Collected from Horses in the Republic of Korea

et al. 2021

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The horse industry has grown rapidly as a leisure industry in the Republic of Korea (ROK) in parallel with an increased demand for equestrian activities. As a result, there has been an increase in horse breeding and equestrian population and potential exposure to ticks and their associated pathogens. To provide a better understanding of the potential disease risks of veterinary and medical importance, a study was conducted to determine the geographical distribution and diversity of ticks collected from horses and vegetation associated with horse racetracks/ranches throughout the ROK. This included a survey of five associated common pathogens, Anaplasma phagocytophilum, Ehrlichia chaffeensis, Borrelia spp., Babesia caballi, and Theileria equi. A total 9220 ticks were collected from horses and associated pastures. Ticks were identified to species, stage of development, and sex. Two species of ticks, Haemaphysalis longicornis (99.9%) and Ixodes nipponensis (0.1%) were identified. Two of the target pathogens, A. phagocytophilum and Borrelia spp., were detected in 5/1409 tick pools (0.35%) and 4/1409 pools (0.28%) of H. longicornis, respectively, both of which are zoonotic pathogens of medical importance. The results of 16S rRNA phylogenetic analysis of A. phagocytophilum showed a close relationship to strains distributed in China, USA, Germany, Italy, Turkey, and Poland. Borrelia spp. showed a close relationship, based on 16S rRNA gene, to the strains reported from the USA (B. burgdorferi and B. americana) and Japan (B. tanukii and B. garinii). These results provide information about the potential risks of veterinary and medical importance and the development of mitigation strategies for disease prevention.

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Section: Discussionsupporting

confidence: 73%

“…Various gene fragments have been used for identification of A. phagocytophilum [ 38 ]. However, other genes, e.g., groEL and msp2 , were shown not to be helpful for the detection of A. phagocytophilum in the ROK [ 37 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection and Phylogenetic Analysis of Tick-Borne Pathogens in Ticks Collected from Horses in the Republic of Korea

et al. 2021

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“…This was the first study reporting the molecular epidemiology of Anaplasmataceae in small ruminants of Uganda, however, further studies using the 16S rRNA and other gene targets related to Anaplasmataceae ( Ali et al., 2017 ; Ringo et al., 2019 ; Seo et al., 2018 ; Shi et al., 2020 ; Yang et al., 2016 ), would pave way for the characterization of Anaplasma infections in both small and large ruminants in Uganda to understand evolutionary changes and sources of variation for improved animal health.…”

Section: Discussionmentioning

confidence: 99%

“…Molecular diagnosis of Anaplasma bacteria is by detection of the 16S rRNA gene ( Ringo et al., 2019 ; Shi et al., 2020 ; Yang et al., 2016 ). This forms the basis of phylogenetic analysis in the Anaplasmataceae ( Seo et al., 2018 ) for insight into genotypic variation and diversity. In Xinjiang, northwest China, a prevalence of 17.6% for A. phagocytophilum, 4.8% for A. bovis and 40.5% for A. ovis shows the importance of these bacteria in small ruminant health ( Yang et al., 2015 ).…”

Section: Introductionmentioning

confidence: 99%

Molecular epidemiology of anaplasmosis in small ruminants along a human-livestock-wildlife interface in Uganda

Kasozi

Welburn

Batiha

et al. 2021

Heliyon

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Background: Information as regards the epidemiology of the Anaplasmataceae in small ruminants in several lowand middle-income countries is scarce. Methods: In this study a total of 712 DNA samples collected from small ruminants were analyzed for Anaplasmataceae and Anaplasma ovis using the 16S rRNA and MSP4 genes respectively. Infection risk was assessed by location, sex and age of the animals and qGIS® was used to construct spatial maps. Results: The prevalence of Anaplasmataceae spp was 89.1% (95% CI: 77.5-95.9) and 79.1% (95% CI: 75.9-82.1) in ovines and caprines respectively (RR ¼ 1.1, 95% CI: 1.0-1.3); higher than those previously reported in other eastern African countries. The prevalence of A. ovis was 26.1% and 25.4% for both ovines and caprines respectively with ovines showing significantly higher levels of infection than caprines (P < 0.05). The risk of Anaplasma ovis infections was not affected by age (OR ¼ 1.2, 95% CI: 0.9-1.7) or sex (OR ¼ 1.1, 95% CI: 0.6-2.0). Small ruminants located at the forest edge (<0.3 km) showed higher A. ovis prevalence than those found inland with infections present in the midland regions associated with increased agricultural activity. Conclusion: Anaplasma ovis remains a major challenge for small ruminant husbandry in Uganda and infections are under-reported. Policy efforts to prioritize management of Anaplasmataceae for small ruminant health would promote livestock productivity in vulnerable communities, improving livelihoods and ecosystem health.

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“…It has been reported that the GroEL and msp2 genes could separately describe A. phagocytophilum. 25 Several studies have demonstrated that the pathogenicity or genotype of A. phagocytophilum would be determined especially by using the groEL gene region. 8,[26][27][28] In the present study, we couldn't unable to replicate horses DNA using groEL fragment, in this way, we couldn't classify A. phagocytophilum with regard to genotype.…”

Section: Discussionmentioning

confidence: 99%

Türkiye’nin Muş İlinde Atlarda Anaplasma phagocytophilum’un İlk Moleküler Tespiti ve Filogenetik Analizi

Oğuz

2021

Kocaeli Üniversitesi Sağlık Bilimleri Dergisi

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Objective: Equine granulocytic anaplasmosis (EGA) caused by Anaplasma phagocytophilum is a disease that generally develop characteristic high fever and a number of changes in blood such as thrombocytopenia. The main transmission of A. phagocytophilum is hard ticks through intrastadially. The aim of this study was to determine the presence of A. phagocytophilum in horses in Muş province of Turkey by using molecular methods. Methods: In the present survey, blood samples were collected in Muş province from 93 horse. Commercial ELISA kit (IgG) and nested-PCR were performed. The DNA was extracted from blood and analysed by nested-PCR technique targeting 16S rRNA gene of A. phagocytophilum and then positive PCR products were sequenced. Results: The seroprevalence against A. phagocytophilum was 8.6% and 6 horses (6.4%) showed positive nested-PCR results. The sequences had 99.5 and 100% homology with the BLAST published A. phagocytophilum amplicons. Conclusion: To the best of our knowledge, this is the first molecular data of A. phagocytophilum in horses in Muş province of Turkey. This study also reveals that A. phagocytophilum isolates isolated from a small region are closely related to A. phagocytophilum isolated from a wide variety of hosts.

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Molecular Detection and Phylogenetic Analysis of Anaplasma phagocytophilum in Horses in Korea

Cited by 12 publications

References 27 publications

Molecular Detection and Phylogenetic Analysis of Tick-Borne Pathogens in Ticks Collected from Horses in the Republic of Korea

Molecular Detection and Phylogenetic Analysis of Tick-Borne Pathogens in Ticks Collected from Horses in the Republic of Korea

Molecular epidemiology of anaplasmosis in small ruminants along a human-livestock-wildlife interface in Uganda

Türkiye’nin Muş İlinde Atlarda Anaplasma phagocytophilum’un İlk Moleküler Tespiti ve Filogenetik Analizi

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