Molecular detection and phylogenetic analysis of avian reticuloendotheliosis virus from formalin fixed tissue by PCR in fancy chicken

Abstract

The present study was carried out to identify avian neoplastic viruses in the formalin fixed tissues of fancy chicken. The tissue samples collected during necropsy were examined by histopathology. It showed lymphoid and reticular cell infiltration in kidney, liver and lungs. For identification and differentiation of avian neoplastic viruses, PCR was performed using primer sets specific for Mareks disease virus, avian leukosis complex and reticuloendotheliosis virus. It was found that tumors were REV originated. Further confirmation, purified PCR product was subjected to sequencing. It showed 99% homology with other REV isolates available in the NCBI database. The present communication describes infection of a fancy chicken with REV on the basis of histopathological findings as well as molecular methods.

Histopathological and Molecular Diagnosis of Avian Reticuloendotheliosis Virus in Commercial Layer Chicken

“…Diffuse severe RE cell infiltration in ovarian stroma, patchy moderate RE cell infiltration in cardiac muscle and perivascular cuffing, gliosis in brain were noticed. The histopathological changes of liver, ovary, heart and brain observed in this study agreed with the findings of earlier workers (Ponnusamy et al, 2018). The PCR test was highly useful for detection of REV virus (Gopal et al, 2012;Ahmed et al, 2018).…”