Molecular detection of a Hepatozoon species in stray cats from a feline colony in North-eastern Spain

Ortuño, Anna; Castellà, Joaquim; Criado–Fornelio, A.; Buling, A.; Barba-Carretero, J.C.

doi:10.1016/j.tvjl.2007.04.009

Cited by 40 publications

(40 citation statements)

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“…However, a higher prevalence of Hepatozoon spp. infection has been described in cats from a colony in the Barcelona area (16%) [18] and in cats with outdoor access in comparison with strictly indoor cats [4, 13]. In our study, positivity to Hepatozoon spp.…”

Section: Discussionsupporting

confidence: 53%

“…A similar higher ratio of H. felis versus H. canis infection in domestic cats was also found in a study from Israel where only two cats out of 55 Hepatozoon -spp.-positive cats were detected with H. canis infection and 53 cats out of a total of 153 tested were positive for H. felis [13]. The Hepatozoon infection prevalences described in previous Spanish studies varied from 0.6% in one study [17], 4% in cats from the Barcelona area [19] and 16% in a cat colony from Barcelona [18], compared to the prevalence in our study of 1.6%. These differences in prevalence rates can be due to the vector distribution, characteristics of the cat populations in each case or differences in the PCR technique and type of sample employed.…”

Section: Discussionmentioning

confidence: 90%

“…It is generally considered that Hepatozoon spp. infection in cats has a low virulence, being mostly subclinical [13, 18], but there is scant clinical information on the disease. Hepatozoon felis usually infects the myocardial and skeletal muscles, with no significant local inflammatory response [13, 34].…”

Section: Discussionmentioning

confidence: 99%

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Molecular detection of Hepatozoon spp. and Cytauxzoon sp. in domestic and stray cats from Madrid, Spain

et al. 2017

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BackgroundDifferent species of apicomplexan protozoans of the genera Hepatozoon and Cytauxzoon can infect domestic cats, but their epidemiology and clinical relevance are not fully understood. The aim of this study was to assess the molecular prevalence of Hepatozoon spp. and Cytauxzoon spp. and to identify associated risk factors and clinical and laboratory abnormalities in a population of cats from Madrid, Spain.MethodsSix hundred and forty-four client-owned and stray cats from Madrid, Spain, were included in this study. DNA samples were analyzed by two polymerase chain reaction (PCR) tests to detect a partial sequence of the 18S rRNA gene of Hepatozoon spp. and Cytauxzoon spp. In order to evaluate possible associations between infection by these protozoans and epidemiological or clinical parameters, data were collected related to: the season of sample collection, age, gender, spayed/neutered status, breed, living area, lifestyle, outdoor access, contact with other animals, prey on wild animals, history of tick or flea infestation, travel history, ectoparasiticide treatment, previous blood transfusion, previous tetracycline administration in the last 60 days, Feline Leukemia virus (FeLV) and Feline Immunodeficiency virus (FIV) status, positivity to other vector-borne diseases, the presence or absence of clinical signs and hematological or biochemical alterations.ResultsDNA of Hepatozoon spp. and Cytauxzoon sp. was amplified from the blood of 10 (1.6%) and 8 (1.2%) cats, respectively. Previous treatment with tetracyclines in the last 60 days, previous administration of blood transfusion, a decrease in haematocrit and an increase in creatinine were associated with Hepatozoon spp. infection. Cytauxzoon sp. infection was more frequent in samples collected during the winter months and in cats living in rural areas. This infection was associated with a FIV-positive status. Some of the cats that were positive for Hepatozoon spp. or Cytauxzoon sp. had been exposed to other vector-borne pathogens, such as Ehrlichia canis and Bartonella henselae.ConclusionsOur results indicate that cats from Madrid, central Spain, are infected with Hepatozoon spp. and Cytauxzoon sp., although with a low prevalence. Further studies are needed to determine the virulence of these agents in Spanish cats.

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Section: Discussionsupporting

confidence: 53%

Section: Discussionmentioning

confidence: 90%

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Molecular detection of Hepatozoon spp. and Cytauxzoon sp. in domestic and stray cats from Madrid, Spain

et al. 2017

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“…Alternatively, however, the natural species diversity of Hepatozoon could in fact be underestimated in that many species may not yet have been found considering their low concern. For a better estimation of the diversity of these haematozoan groups and correct species identification, molecular techniques have now been widely applied (Wicks et al 2006, Rubini et al 2006, Boulianne et al 2007, Ortuño et al 2008, Vilcins et al 2009Tomé et al 2012Tomé et al , 2013. In the present work, we successfully amplified the partial fragment of the 18S rRNA gene and constructed a phylogenetic tree based on this molecular marker.…”

Section: Discussionmentioning

confidence: 95%

Redescription of Haemogregarina garnhami (Apicomplexa: Adeleorina) from the blood of Psammophis schokari (Serpentes: Colubridae) as Hepatozoon garnhami n. comb. based on molecular, morphometric and morphologic characters

Abdel‐Baki¹,

Al‐Quraishy²,

Zhang³

2014

Acta Parasitologica

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Hepatozoon garnhami n. comb. was redescribed from Schokari sand snakes (Psammophis schokari) collected from Riyadh city in Saudi Arabia. Gametocytes were found in the peripheral blood of 2 of 15 snakes examined. Based on the similar morphological and morphometric characteristics, the same host and a similar host habitat environment, it can be concluded for the first time that the present species is conspecific with Haemogregarina garnhami previously reported from Psammophis shokari aegyptius. To further characterize this parasite, the partial 18S rRNA gene was amplified and sequenced. The sequence analysis also showed that Haemogregarina garnhami should be reassigned into the genus Hepatozoon as Hepatozoon garnhami which has 99.5% (859/863 bp) sequence similarity to Hepatozoon ayorgbor, infecting the erythrocytes of Python regius in Ghana. Phylogenetic analysis showed that H. garnhami formed a mixed clade with Hepatozoon spp. from geckos, snakes and rodents and ophidian Hepatozoon spp. did not form a separated phylogenetic unit. Also, Psammophis schokari-infecting Hepatozoon contained several different genetic lineages. To our knowledge, the present work extends the geographic distribution of H. garnhami and is the first report of Hepatozoon infection in snakes from Saudi Arabia.

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“…Few molecular studies have been done concerning the molecular characterization of Hepatozoon species among domestic and wild felids (Criado-Fornelio et al 2006;Rubini et al 2006;Metzger et al 2008;Jittapalapong et al 2006;Ortuño et al 2007;Salakij et al 2008;André et al 2010). The present work aimed to characterize molecularly the presence of Hepatozoon sp.…”

mentioning

confidence: 99%

Molecular characterization of Hepatozoon sp. in cats from São Luís Island, Maranhão, Northeastern Brazil

et al. 2011

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Few molecular studies have been done concerning the molecular characterization of Hepatozoon species among domestic and wild felids. The present work aimed to characterize molecularly the presence of Hepatozoon sp. DNA in cat blood samples from São Luís Island, Maranhão state, Northeastern Brazil. EDTA-whole blood samples were collected from 200 domestic cats with outdoor and wood areas access from São Luís, Maranhão, Brazil. Each sample of extracted DNA was used as a template in PCR reactions aiming to amplify a partial sequence of 18S rRNA of Hepatozoon spp. We also performed sequence alignment to establish the identity of the parasite species infecting these animals using DNA sequences based on 18S rRNA. From 200 sampled cats, Hepatozoon DNA was only found in one animal (0.5%). The found Hepatozoon DNA showed 97% of identity with Hemobartonella felis isolates 1 and 2 from Spain. When analyzing the phylogenetic tree, the found Hepatozoon DNA was in the same clade than H. felis isolates. Our findings suggest that more than one species of Hepatozoon could infect felids in Brazil.

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Molecular detection of a Hepatozoon species in stray cats from a feline colony in North-eastern Spain

Cited by 40 publications

References 3 publications

Molecular detection of Hepatozoon spp. and Cytauxzoon sp. in domestic and stray cats from Madrid, Spain

Molecular detection of Hepatozoon spp. and Cytauxzoon sp. in domestic and stray cats from Madrid, Spain

Redescription of Haemogregarina garnhami (Apicomplexa: Adeleorina) from the blood of Psammophis schokari (Serpentes: Colubridae) as Hepatozoon garnhami n. comb. based on molecular, morphometric and morphologic characters

Molecular characterization of Hepatozoon sp. in cats from São Luís Island, Maranhão, Northeastern Brazil

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