2020
DOI: 10.36103/ijas.v51ispecial.897
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Molecular Detection of Astrovirus Using Reverse Transcriptase Quantitative Polymerase Chain Reaction Technique

Abstract: This study was aimed to highlight the importance of the melt curve-quantitative reverse transcriptase PCR (RT-qPCR) analysis in the detection of astrovirus (AstV) from both negative and positive rotavirus and enterovirus (EVs) samples, and the effectiveness of the AstV infection on the vaccine immunogenicity in the vaccinated infected children.  By RT-qPCR based Sybre green associated- melting curve assay, stool samples of 49 enterovirus suspected patients and of 39 rotavirus suspected patients were tested for… Show more

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