Molecular Detection of Avian Influenza Virus in Wild Birds in Morocco, 2016–2019

Mellouli, Fatiha El; Abouchoaib, Nabil; Zekhnini, Hasnae; Khayli, Mounir; Fusaro, Alice; Idrissi, Hamid Rguibi; Benhoussa, Abdelaziz

doi:10.1637/aviandiseases-d-21-00070

Cited by 6 publications

(8 citation statements)

References 50 publications

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“…Moreover, the presence of LPAI H9N2 infection among the backyard chicken population reported in our study makes it an additional risk factor for the poultry industry. It indicates that this population might represent the transmission of the virus between wild birds and domesticated birds in both directions [ 30 ]. Furthermore, the ability of LPAI H9N2 to infect and be transmitted to both chickens and humans highlights the importance of characterizing the circulating strains in poultry.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of major infectious diseases in backyard chickens from rural markets in Morocco

Fagrach,

Arbani,

Karroute

et al. 2023

Vet World

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Background and Aim: Raising backyard chickens is a common practice in Morocco, mainly in rural or periurban areas. Constraints due to devastating avian diseases have been recognized as a major limiting factor in backyard poultry production. Consequently, these flocks could potentially be implicated as reservoirs for poultry diseases. However, there is a considerable lack of information on disease prevalence in this production system, and the risk represented by these small flocks remains under debate. This study aimed to estimate the seroprevalence and identify related risk factors of a range of bacterial and viral pathogens of outstanding importance for the economy and public health in backyard poultry in Morocco. Materials and Methods: : A total of 712 sera samples and 258 cloacal swabs were collected from 712 backyard chickens from 15 rural markets in the Khemisset and Skhirat-Temara provinces. None of the sampled chickens received any vaccination. Sera samples were screened for antibodies against Newcastle disease virus (NDV) and low pathogenic avian influenza H9N2 subtype (LPAI H9N2) using a hemagglutination-inhibition test, against bursal infectious disease virus (IBDV) and infectious bronchitis virus (IBV) using enzyme-linked immunosorbent assay, and against Mycoplasma gallisepticum (MG) and Mycoplasma synoviae (MS) using a rapid serum agglutination test. Swab samples were compiled into 86 pools and submitted for molecular detection using real-time reverse-transcription-polymerase chain reaction (RT-PCR). Results: The seroprevalences in backyard chickens for NDV, LPAI H9N2, IBDV, IBV, MG, and MS were 52.1% (371/712), 63.5% (452/712), 84.7% (603/712), 82.2% (585/712), 58% (413/712), and 74.8% (533/712), respectively. Based on the RT-PCR results, 2.3% (2/86), 62.8% (54/86), 2.3% (2/86), 63.9% (55/86), 40.7% (35/86), and 29.1% (25/86) of the pools were positive for NDV, H9N2 LPAI, IBDV, IBV, MG, and MS, respectively. Multiple coinfections (H9N2-IBV-MG), (H9N2-IBV-MS), or (IBV-MG-MS) were observed in 15.1%, 8.5%, and 8.5% of the tested samples, respectively. Conclusion: The results show that backyard chicken flocks and rural markets have the potential to serve as reservoirs or amplifiers for poultry pathogens and could pose a risk to the commercial poultry sector. This highlights the need for a comprehensive and adapted vaccination plan for backyard chickens, and extension of efforts to increase flock owners’ awareness of avian diseases and incite the implementation of biosecurity measures at the farm level. Keywords: avian diseases, backyard chickens, low pathogenic avian influenza H9N2, Newcastle disease, risk factors, rural markets.

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Section: Discussionmentioning

confidence: 99%

Prevalence of major infectious diseases in backyard chickens from rural markets in Morocco

Fagrach,

Arbani,

Karroute

et al. 2023

Vet World

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“…In our study, the species of migratory birds were mostly from Anseriformes (i.e., pintails, common teals, Indian spot-billed ducks, northern shovelers, Eurasian wigeons, and tufted ducks), Charadriiformes (i.e., black-winged stilts and pied avocets), and Pelecaniformes (i.e., black-faced spoonbills). 60 Anseriformes, Charadriiformes, and Ardeidae were regarded as natural reservoirs for AIV. 12 The prevalence of influenza A virus in mallards and common teals (7.2%) was higher than that in Eurasian wigeons, northern pintails, gadwalls, and northern shovelers (3%).…”

Section: ■ Discussionmentioning

confidence: 99%

“…In our observation, the number of pintails, common teals, Indian spot-billed ducks, Eurasian wigeons, tufted ducks, pied avocets, black-faced spoonbills, and great cormorants had a significant positive correlation with H9, and the number of common teals, northern shovelers, Eurasian wigeons, pied avocets, and black-faced spoonbills had a significant positive correlation with H7. In our study, the species of migratory birds were mostly from Anseriformes (i.e., pintails, common teals, Indian spot-billed ducks, northern shovelers, Eurasian wigeons, and tufted ducks), Charadriiformes (i.e., black-winged stilts and pied avocets), and Pelecaniformes (i.e., black-faced spoonbills) . Anseriformes, Charadriiformes, and Ardeidae were regarded as natural reservoirs for AIV .…”

Section: Discussionmentioning

confidence: 99%

Airborne Avian Influenza Virus in Ambient Air in the Winter Habitats of Migratory Birds

Zhang

Chen

Lin

et al. 2022

Environ. Sci. Technol.

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Outbreaks of avian influenza virus (AIV) have raised public concerns recently. Airborne AIV has been evaluated in live poultry markets and case farms; however, no study has discussed airborne AIV in ambient air in the winter habitats of migratory birds. Therefore, this study aimed to evaluate airborne AIV, specifically H5, H7, and H9, in a critical winter habitat of migratory birds and assess the factors influencing airborne AIV transmission in ambient air to provide novel insights into the epidemiology of avian influenza. A total of 357 ambient air samples were collected in the Aogu Wetland, Taiwan, Republic of China, between October 2017 and December 2019 and analyzed using quantitative real-time polymerase chain reaction. The effects of environmental factors including air pollutants, meteorological factors, and the species of the observed migratory birds on the concentration of airborne AIV were also analyzed. To our knowledge, this is the first study to investigate the relationship between airborne AIV in ambient air and the influence factors in the winter habitats of migratory birds, demonstrating the benefits of environmental sampling for infectious disease epidemiology. The positive rate of airborne H7 (12%) was higher than that of H5 (8%) and H9 (10%). The daily mean temperature and daily maximum temperature had a significant negative correlation with influenza A, H7, and H9. Cold air masses and bird migration were significantly associated with airborne H9 and H7, respectively. In addition, we observed a significant correlation between AIV and the number of pintails, common teals, Indian spot-billed ducks, northern shovelers, Eurasian wigeons, tufted ducks, pied avocets, black-faced spoonbills, and great cormorants. In conclusion, we demonstrated the potential for alternative surveillance approaches (monitoring bird species) as an indicator for influenza-related risks and identified cold air masses and the presence of specific bird species as potential drivers of the presence and/or the airborne concentration of AIV.

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“…Hence, the circulation of H9N2 AIV should be investigated in other species, such as wild birds and mammals, including humans, to better understand virus ecology in Morocco. All the more so as a recent molecular study on the presence of the AIV in wild birds staying in the corridor of wetlands during their winter crossing in Morocco, showed a high positivity-rate of the influenza virus in different birds species [ 77 ]. Continuous systematic surveillance of molecular evolution of H9N2 AIV is strongly needed to anticipate the emergence of novel H9N2 strains escaping vaccine protection or with an increased human pandemic potential.…”

Section: Discussionmentioning

confidence: 99%

Spatiotemporal Dynamics, Evolutionary History and Zoonotic Potential of Moroccan H9N2 Avian Influenza Viruses from 2016 to 2021

Mellouli

Mouahid²,

Fusaro

et al. 2022

Viruses

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The H9N2 virus continues to spread in wild birds and poultry worldwide. At the beginning of 2016, the H9N2 Avian influenza virus (AIV) was detected in Morocco for the first time; despite the implementation of vaccination strategies to control the disease, the virus has become endemic in poultry in the country. The present study was carried out to investigate the origins, zoonotic potential, as well as the impact of vaccination on the molecular evolution of Moroccan H9N2 viruses. Twenty-eight (28) H9N2 viruses collected from 2016 to 2021 in Moroccan poultry flocks were isolated and their whole genomes sequenced. Phylogenetic and evolutionary analyses showed that Moroccan H9N2 viruses belong to the G1-like lineage and are closely related to viruses isolated in Africa and the Middle East. A high similarity among all the 2016–2017 hemagglutinin sequences was observed, while the viruses identified in 2018–2019 and 2020–2021 were separated from their 2016–2017 ancestors by long branches. Mutations in the HA protein associated with antigenic drift and increased zoonotic potential were also found. The Bayesian phylogeographic analyses revealed the Middle East as being the region where the Moroccan H9N2 virus may have originated, before spreading to the other African countries. Our study is the first comprehensive analysis of the evolutionary history of the H9N2 viruses in the country, highlighting their zoonotic potential and pointing out the importance of implementing effective monitoring systems.

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Molecular Detection of Avian Influenza Virus in Wild Birds in Morocco, 2016–2019

Cited by 6 publications

References 50 publications

Prevalence of major infectious diseases in backyard chickens from rural markets in Morocco

Prevalence of major infectious diseases in backyard chickens from rural markets in Morocco

Airborne Avian Influenza Virus in Ambient Air in the Winter Habitats of Migratory Birds

Spatiotemporal Dynamics, Evolutionary History and Zoonotic Potential of Moroccan H9N2 Avian Influenza Viruses from 2016 to 2021

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