Molecular Detection of Bartonella Species in Ticks From Peru

Billeter, Sarah A.; Cáceres, Abraham G.; Gonzales-Hidalgo, James; Luna-Caypo, Deysi; Kosoy, Michael

doi:10.1603/me10240

Cited by 18 publications

(13 citation statements)

References 27 publications

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“…The human flea is an aggressive and promiscuous ectoparasite found on a wide range of hosts, including domestic dogs [48], [49]. In addition, B. rochalimae was also recently detected in a brown dog tick ( Rhipicephalus sanguineus ) from a dog in the province of Callao [45], located only 25 km (15.5 miles) northwest of San Juan de Miraflores, the site where the largest number of dogs infected with B. rochalimae was detected in our study. Both Callao and San Juan de Miraflores are suburbs of the country's capital (Lima), where human and canine population densities and social and economic conditions may favor the multiplication and dissemination of vectors, increasing the risk of human infection.…”

Section: Discussionsupporting

confidence: 60%

“…Infections with B. rochalimae or B. v. berkhoffii have never been described in humans or animals from Peru, even though other known and unknown species of Bartonella have been described from small mammals, bats, body lice, fleas and ticks from Peru [22], [42]–[45]. In our study, we documented 10.3% of dogs infected with B. rochalimae and 7.7% of dogs infected with B. v. berkhoffii from Caraz and Huaraz.…”

Section: Discussionmentioning

confidence: 53%

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Infection of Domestic Dogs in Peru by Zoonotic Bartonella Species: A Cross-Sectional Prevalence Study of 219 Asymptomatic Dogs

et al. 2013

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Bartonella species are emerging infectious organisms transmitted by arthropods capable of causing long-lasting infection in mammalian hosts. Among over 30 species described from four continents to date, 15 are known to infect humans, with eight of these capable of infecting dogs as well. B. bacilliformis is the only species described infecting humans in Peru; however, several other Bartonella species were detected in small mammals, bats, ticks, and fleas in that country. The objective of this study was to determine the serological and/or molecular prevalence of Bartonella species in asymptomatic dogs in Peru in order to indirectly evaluate the potential for human exposure to zoonotic Bartonella species. A convenient sample of 219 healthy dogs was obtained from five cities and three villages in Peru. EDTA-blood samples were collected from 205 dogs, whereas serum samples were available from 108 dogs. The EDTA-blood samples were screened by PCR followed by nucleotide sequencing for species identification. Antibodies against B. vinsonii berkhoffii and B. rochalimae were detected by IFA (cut-off of 1∶64). Bartonella DNA was detected in 21 of the 205 dogs (10%). Fifteen dogs were infected with B. rochalimae, while six dogs were infected with B. v. berkhoffii genotype III. Seropositivity for B. rochalimae was detected in 67 dogs (62%), and for B. v. berkhoffii in 43 (40%) of the 108 dogs. Reciprocal titers ≥1∶256 for B. rochalimae were detected in 19% of dogs, and for B. v. berkhoffii in 6.5% of dogs. This study identifies for the first time a population of dogs exposed to or infected with zoonotic Bartonella species, suggesting that domestic dogs may be the natural reservoir of these zoonotic organisms. Since dogs are epidemiological sentinels, Peruvian humans may be exposed to infections with B. rochalimae or B. v. berkhoffii.

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Section: Discussionsupporting

confidence: 60%

Section: Discussionmentioning

confidence: 53%

Infection of Domestic Dogs in Peru by Zoonotic Bartonella Species: A Cross-Sectional Prevalence Study of 219 Asymptomatic Dogs

et al. 2013

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“…While reviewing recently published literature relative to potential transmission of Bartonella species by ticks [20-24], the mother, a 58-year-old anesthesiologist from the Netherlands contacted one of the investigators in our laboratory (EBB) by email and requested BAPGM enrichment blood culture testing to determine if she and her son could have been infected with a Bartonella sp. following tick bites.…”

Section: Methodsmentioning

confidence: 99%

Bartonella henselae bacteremia in a mother and son potentially associated with tick exposure

et al. 2013

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BackgroundBartonella henselae is a zoonotic, alpha Proteobacterium, historically associated with cat scratch disease (CSD), but more recently associated with persistent bacteremia, fever of unknown origin, arthritic and neurological disorders, and bacillary angiomatosis, and peliosis hepatis in immunocompromised patients. A family from the Netherlands contacted our laboratory requesting to be included in a research study (NCSU-IRB#1960), designed to characterize Bartonella spp. bacteremia in people with extensive arthropod or animal exposure. All four family members had been exposed to tick bites in Zeeland, southwestern Netherlands. The mother and son were exhibiting symptoms including fatigue, headaches, memory loss, disorientation, peripheral neuropathic pain, striae (son only), and loss of coordination, whereas the father and daughter were healthy.MethodsEach family member was tested for serological evidence of Bartonella exposure using B. vinsonii subsp. berkhoffii genotypes I-III, B. henselae and B. koehlerae indirect fluorescent antibody assays and for bacteremia using the BAPGM enrichment blood culture platform.ResultsThe mother was seroreactive to multiple Bartonella spp. antigens and bacteremia was confirmed by PCR amplification of B. henselae DNA from blood, and from a BAPGM blood agar plate subculture isolate. The son was not seroreactive to any Bartonella sp. antigen, but B. henselae DNA was amplified from several blood and serum samples, from BAPGM enrichment blood culture, and from a cutaneous striae biopsy. The father and daughter were seronegative to all Bartonella spp. antigens, and negative for Bartonella DNA amplification.ConclusionsHistorically, persistent B. henselae bacteremia was not thought to occur in immunocompetent humans. To our knowledge, this study provides preliminary evidence supporting the possibility of persistent B. henselae bacteremia in immunocompetent persons from Europe. Cat or flea contact was considered an unlikely source of transmission and the mother, a physician, reported that clinical symptoms developed following tick exposure. To our knowledge, this is the first time that a B. henselae organism has been visualized in and amplified from a striae lesion. As the tick bites occurred three years prior to documentation of B. henselae bacteremia, the mode of transmission could not be determined.

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“…En el Perú se ha investigado si existen otros artrópodos vectores de Bartonella bacilliformis, pero hasta el momento parece ser que solo los flebotomíneos del género Lutzomyia son los únicos que transmiten la enfermedad de Carrión (75) .…”

Section: Otros Potenciales Vectores De La Enfermedad De Carrión En Elunclassified

Vectores de la leishmaniasis tegumentaria y la Enfermedad de Carrión en el Perú: una actualización

Zorrilla

Vásquez

Espada

et al. 2017

Rev Peru Med Exp Salud Publica

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Molecular Detection of Bartonella Species in Ticks From Peru

Cited by 18 publications

References 27 publications

Infection of Domestic Dogs in Peru by Zoonotic Bartonella Species: A Cross-Sectional Prevalence Study of 219 Asymptomatic Dogs

Infection of Domestic Dogs in Peru by Zoonotic Bartonella Species: A Cross-Sectional Prevalence Study of 219 Asymptomatic Dogs

Bartonella henselae bacteremia in a mother and son potentially associated with tick exposure

Vectores de la leishmaniasis tegumentaria y la Enfermedad de Carrión en el Perú: una actualización

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