Molecular detection of Blastocystis in black bears and sika deer in northern China

Ni, Hong-Bo; Gong, Qing‐Long; Zhang, Nian‐Zhang; Zhao, Quan; Tao, Wei-Fu; Qiu, Hongming; Fei, Yu-Chao; Zhang, Xiaoxuan

doi:10.1007/s00436-021-07068-0

Cited by 7 publications

(10 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The overall prevalence of Blastocystis in M. chrysogaster (39.8%, 80/201) was higher than that reported in reindeer in Heilongjiang province (6.7%), sika deer in Jilin province (1.3%), and water deer in Korea (20.8%) (Table 3) (Kim et al, 2020;Ni et al, 2021;Wang et al, 2018). Notably, major prevalences, such as 50.0% and 100%, were occasionally found in different deer species, although sample sizes were extremely small (Table 3) (Alfellani et al, 2013;Betts et al, 2018;Zhao et al, 2018).…”

Section: Discussionmentioning

confidence: 82%

“…Proteromonas lacertae was the outgroup. Bar = substitutions/site Li et al, 2019;Ni et al, 2021;Wang et al, 2018;Zhao et al, 2018). Among them, ST10 was the most common ST found in deer (Table 3), and it may be the host-adapted ST for animals within the Cervidae family.…”

Section: Discussionmentioning

confidence: 99%

“…Previously, following the survey of at least 12 Cervidae species, 8 Blastocystis STs were reported, including ST2, ST4, ST5, ST10, ST13, ST14, ST21, and ST24 (Table 3) (Alfellani et al, 2013; Betts et al, 2018; Kim et al, 2020; Li et al, 2019; Maloney & Santín, 2021; Ni et al, 2021; Wang et al, 2018; Zhao et al, 2018). Among them, ST10 was the most common ST found in deer (Table 3), and it may be the host‐adapted ST for animals within the Cervidae family.…”

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Genetic diversity of Blastocystis subtypes in the Alpine musk deer (Moschus chrysogaster) in Gansu province, northwestern China

Wang

Liu

et al. 2022

J Eukaryotic Microbiology

View full text Add to dashboard Cite

Blastocystis sp. is a gastrointestinal pathogen that is frequently found in humans and animals worldwide. In this study, 201 fecal samples were collected from captive Alpine musk deer (Moschus chrysogaster) at three farms in Gansu province. Blastocystis was detected and subtyped by amplifying and sequencing the small subunit ribosomal DNA gene. The overall prevalence of Blastocystis was 39.8% (80/201). Five known Blastocystis subtypes (STs), including ST1 (n = 1), ST4 (n = 12), ST10 (n = 50), ST14 (n = 6), and ST24 (n = 11) were identified using subtyping and evolutionary analysis. ST10 was the most common ST observed in each farm. This study showed the infection status and genetic characteristics of Blastocystis in M. chrysogaster. Based on the surveyed data, because various potentially zoonotic STs, such as ST1, ST4, ST10, ST14, and ST24, were detected, it is believed that the zoonotic risk of Blastocystis from the Alpine musk deer in this area cannot be ignored.

show abstract

Section: Discussionmentioning

confidence: 82%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic diversity of Blastocystis subtypes in the Alpine musk deer (Moschus chrysogaster) in Gansu province, northwestern China

Wang

Liu

et al. 2022

J Eukaryotic Microbiology

View full text Add to dashboard Cite

show abstract

“…in forest musk deer examined in this study was 14.7% (74/504), which is lower than that previously found in wild takin in China (57.1%, 28/49) [56], wild Père David's deer in China (56.3%, 72/128) [29], wild Korean water deer in Korea (40.8%, 51/125) [21], farmed Alpine musk deer in northwestern China (39.8%, 80/201) [49], farmed goats in Malaysia (30.9%, 73/236) [45], and farmed camels in Libya (24%, 47/196) [3]. However, the prevalence is higher than that observed in farmed sika deer in northeastern China (14.6%, 12/82) [47], wild reindeer in China (6.73%, 7/104) [48], farmed sika deer in northern China (0.8%, 6/760) [30], and farmed goats in Nepal (0.75%, 3/400) [15]. The reason for the different prevalence of Blastocystis sp.…”

Section: Discussionmentioning

confidence: 95%

Occurrence, genetic diversity and zoonotic potential of Blastocystis sp. in forest musk deer (Moschus berezovskii) in Southwest China

et al. 2022

View full text Add to dashboard Cite

Blastocystis sp. is a common anaerobic protist with controversial pathogenicity that can infect various animals and humans. However, there are no reports of Blastocystis sp. infections in forest musk deer (Moschus berezovskii). The present study was designed to examine the occurrence, subtype distribution and genetic characterization of Blastocystis sp. in forest musk deer in southwestern China, and to assess the potential for zoonotic transmission. A total of 504 fresh stool samples were collected from captive forest musk deer in four distinct areas of southwestern China. Overall, 14.7% of the forest musk deer (74/504) were found to be infected with Blastocystis sp. The highest occurrence of Blastocystis sp. was observed in Dujiangyan (27.5%), followed by Maerkang (23.3%). The occurrence of Blastocystis sp. was 7.9% and 4.1% in Shimian and Hanyuan, respectively. Significant differences in the occurrence of Blastocystis sp. among different areas were observed (p < 0.05), while we did not observe significant differences among animals of different age and sex (p > 0.05). Two known zoonotic subtypes (ST1 and ST5) and three animal-predominant subtypes (ST10, ST13, and ST14) were identified, of which ST10 was the most common (36/74, 48.6%). Our findings highlight that forest musk deer may be potential reservoirs of zoonotic human Blastocystis sp. infections.

show abstract

“…Exposure to infective cysts of Blastocystis from the feces of Asiatic black bears poses a potential risk to other animals and public health because of the high-density feeding environment in farms and zoos. Currently, there are only two reports describing Blastocystis infection in bears from Heilongjiang and Sichuan Provinces in China ( Ni et al., 2021 ; Deng et al., 2021 ). This study thus aimed to determine the infection rates and subtype distributions of Blastocystis in confined Asiatic black bears through PCR amplification and analysis of the Santín regions of the SSU rRNA gene.…”

Section: Introductionmentioning

confidence: 99%

Molecular identification and subtype analysis of Blastocystis in captive Asiatic black bears (Ursus thibetanus) in China’s Heilongjiang and Fujian provinces

Zhou

Zhao²,

Yang³

et al. 2022

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Blastocystis sp. is the most isolated enteric protozoan in parasitological surveys of humans. A substantial percentage of human infections is attributed to zoonotic transmissions. However, the contribution of each animal source to human infections with blastocystis is not yet fully understood. This study thus aimed to determine the infection rates and subtype distributions of Blastocystis sp. in captive Asiatic black bears (Ursus thibetanus) in China’s Heilongjiang and Fujian provinces. A total of 218 fresh fecal specimens were collected from captive Asiatic black bears in Heilongjiang (n = 36) and Fujian (n = 182) between May 2015 and December 2017. Genomic DNA was extracted from each sample and then examined for Blastocystis through SSU rRNA gene amplicon-based sequencing. A phylogenetic tree based on the Blastocystis positive sequences was reconstructed using the Mega X program. Eleven percent (24/218) of the animals had Blastocystis and six Blastocystis subtypes, including ST4 (n = 14), ST10 (n = 3), ST1 (n = 2), ST2 (n = 1), ST5 (n = 1), and ST12 (n = 1) were identified. A total of 14 representative sequences, including seven sequences that have been described previously and seven novel sequences comprising ST10 (n = 2), ST5 (n = 1), and ST4 (n = 4), were obtained from the six subtypes of Blastocystis. This study is the first to report the presence of Blastocystis in captive Asiatic black bears in Fujian, China. It provides baseline data for controlling and preventing Blastocystis infection in farm communities. Zoonotic infections in bears with ST1, ST2, ST4, ST5, ST10, and ST14 should be considered potential public health threats. The novel ST sequences of Blastocystis generated in this study provide novel insights into the genotypic variation within the Blastocystis sp.

show abstract

Molecular detection of Blastocystis in black bears and sika deer in northern China

Cited by 7 publications

References 31 publications

Genetic diversity of Blastocystis subtypes in the Alpine musk deer (Moschus chrysogaster) in Gansu province, northwestern China

Genetic diversity of Blastocystis subtypes in the Alpine musk deer (Moschus chrysogaster) in Gansu province, northwestern China

Occurrence, genetic diversity and zoonotic potential of Blastocystis sp. in forest musk deer (Moschus berezovskii) in Southwest China

Molecular identification and subtype analysis of Blastocystis in captive Asiatic black bears (Ursus thibetanus) in China’s Heilongjiang and Fujian provinces

Contact Info

Product

Resources

About