Molecular detection of Candida species from hospitalized patients’ specimens

Camacho-Cardoso, José Luis; Martínez-Rivera, María Ángeles; Manzano-Gayosso, Patricia; Méndez-Tovar, Luis Javier; López-Martínez, Rubén; Hernández-Hernández, Francisca

doi:10.24875/gmm.m18000045

Cited by 4 publications

(3 citation statements)

References 29 publications

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“…The incidence of these species agrees with a previous study carried out in Honduras where 167 cultures were evaluated, with C. albicans , C. tropicalis , and C. glabrata being the most commonly isolated species [ 25 ]. Similar results have been reported in Mexico and India [ 27 , 31 , 32 ].…”

Section: Discussionsupporting

confidence: 90%

Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris?

Ortiz¹,

Aguilar²,

Galindo³

et al. 2022

CMM

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Background and Purpose: Infections by emerging and multiresistant Candida species are becoming more frequent throughout the world. This study aimed to describe Candida species in different wards of a tertiary hospital in Honduras. Materials and Methods: The prevalence of species within the C. albicans complex was estimated using a molecular approach, and C. auris was investigated using a yeast poolbased DNA extraction method. In total, 328 yeast isolates were identified using phenotypic approaches. For the identification of species within the albicans complex, a molecular approach based on the size polymorphisms of the hpw1 gene was used. In addition, a technique was optimized based on DNA extraction in pools for the rapid identification of C. auris. Results: A total of 11 species of Candida were identified in the hospital wards. C. albicans showed the highest number of isolates (52.4%). Within the C. albicans complex, C. albicans sensu stricto was the most common, followed by C. dubliniensis. However, C. auris was not found. Conclusion: Reports on the distribution of Candida species in Honduras are limited; accordingly, the data from this study are of importance for a better understanding of their epidemiology. Moreover, a simple method was offered for the detection of C. auris that could help in its detection in low-resource settings.

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Section: Discussionsupporting

confidence: 90%

Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris?

Ortiz¹,

Aguilar²,

Galindo³

et al. 2022

CMM

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“…Candidiasis is a fungal infection caused by Candida species that features various clinical expressions, including superficial, mucocutaneous, and invasive infection (Camacho-Cardoso et al, 2017). Although Candida species are commensal organisms that presented in healthy individuals and in the natural environment, some of these fungi can become opportunistic pathogens induced by alteration in the host environment, especially in immunocompromised patients (Sadeghi et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Probe-based qPCR Assay for Rapid Detection of Predominant Candida glabrata Sequence Type in Korea

Bae

Lee

Jin

2019

BSL

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Recent years have seen an increase in the incidence of candidiasis caused by non-albicans Candida (NAC) species. In fact, C. glabrata is now second only to C. albicans as the most common cause of invasive candidiasis. Therefore, the rapid genotyping specifically for C. glabrata is required for early diagnosis and treatment of candidiasis. A number of genotyping assays have been developed to differentiate C. glabrata sequence types (STs), but they have several limitations. In the previous study, multi-locus sequence typing (MLST) has performed with a total of 101 C. glabrata clinical isolates to analyze the prevalent C. glabrata STs in Korea. A total of 11 different C. glabrata STs were identified and, among them, ST-138 was the most commonly classified. Thus, a novel probe-based quantitative PCR (qPCR) assay was developed and evaluated for rapid and accurate identification of the predominant C. glabrata ST-138 in Korea. Two primer pairs and hybridization probe sets were designed for the amplification of internal transcribed spacer 1 (ITS1) region and TRP1 gene. Analytical sensitivity of the probe-based qPCR assay was 100 ng to 10 pg and 100 ng to 100 pg (per 1 μL), which target ITS1 region and TRP1 gene, respectively. This assay did not react with any other Candida species and bacteria except C. glabrata. Of the 101 clinical isolates, 99 cases (98%) were concordant with MLST results. This novel probebased qPCR assay proved to be rapid, sensitive, highly specific, reproducible, and cost-effective than other genotyping assay for C. glabrata ST-138 identification.

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“…A second study analyzed 5 different types of clinical samples in Iran, and showed that C. albicans was the predominant species, followed by C. glabrata and C. tropicalis [27]. Another study conducted with eleven types of clinical samples from hospitalized patients with suspected fungal infection in Mexico City showed a higher prevalence of C. albicans, followed by C. tropicalis and C. glabrata [28].…”

Section: Comparison Of Microscan ® Hardychrom ® and Pcr-rflpmentioning

confidence: 99%

Identification of <em>Candida</em> Species from Clinical Samples in a Honduran Tertiary Hospital

Montes

Ortiz

Galindo

et al. 2019

Preprint

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Candida species are one of the most important causes of human infections, especially in hospitals and among immunocompromised patients. The correct and rapid etiological identification of yeast infections is important to provide adequate therapy, reduce mortality and control outbreaks. In this study, Candida species were identified in patients with suspected fungal infection, and phenotypic and genotypic identification methods were compared. A total of 167 axenic fungal cultures and 46 clinical samples were analyzed by HardyCHROM®, MicroScan®, and PCR-RFLP. The species of the C. albicans complex were the most frequent, followed by C. tropicalis and C. glabrata. Less common but clinically relevant species of Candida were also isolated. The comparison between the three methods was concordant, especially for the most common Candida species. Fungal DNA amplification was successful in all clinical samples.

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Molecular detection of Candida species from hospitalized patients’ specimens

Cited by 4 publications

References 29 publications

Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris?

Candida species isolated from clinical samples in a tertiary hospital in Honduras: Where is Candida auris?

Probe-based qPCR Assay for Rapid Detection of Predominant Candida glabrata Sequence Type in Korea

Identification of <em>Candida</em> Species from Clinical Samples in a Honduran Tertiary Hospital

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