Molecular detection of Coxiella-like endosymbionts in Rhipicephalus microplus from north India

Rialch, Ajayta; Sankar, M.; Silamparasan, M.; Madhusoodan, A.P.; Kharayat, Nitish Singh; Gautam, Siddharth; Gurav, Amol; Thankappan, Sabrinath

doi:10.1016/j.vprsr.2022.100803

Cited by 2 publications

(5 citation statements)

References 36 publications

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“…It was reported that Wolbachia gains entry into the ticks through incidental infection of ticks by a parasitic wasp Ixodiphagus hookeri, which is responsible for detection of Wolbachia in ticks (19). In addition, the lack of Francisella-like endosymbionts in our study is in line with the reports of Rialch et al (2022) (15). Gerhart et al (2016) (20) reported that Francisella-like endosymbionts could be detected in ticks infesting rodents.…”

Section: Discussionsupporting

confidence: 92%

“…Our finding of CLEs in R. sanguineus is similar to the reports of Lalzer et al (2012) (13) and Duron et al (2018)(5). In addition, such reports of CLEs in R. sanguineus and R. microplus have been reported from Northeast India(14) and Uttarakhand, North India(15). BLAST analysis revealed that the CLEs detected in R. sanguineus had 99.78-100% similarity with the sequences reported in R. sanguineus from Thailand(16), and Australia(17).…”

supporting

confidence: 92%

“…The genetic similarity of CLEs detected from the adult females, eggs, and larvae further confirms the transovarial transmission of CLEs in R. sanguineus. Though there are reports on the high prevalence of CLEs in female ticks, in our study we observed that both the male and female ticks harbored CLEs, which might be due to the vertical transmission of CLEs (2,15).…”

Section: Discussioncontrasting

confidence: 42%

“…Rialch et al (2022) (15), demonstrated the transovarial transmission of endosymbionts in Rhipicephalus microplus ticks.…”

mentioning

confidence: 99%

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Detection and Genotyping of Coxiella-like Endosymbionts in Rhipicephalus and Haemaphysalis Ticks Infesting Pets and Livestock in Puducherry, India

Devaraju,

Gopal,

Arif

et al. 2024

J Bacteriol Virol.

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Ticks are obligate blood-feeders that are vectors for several pathogens of public health and veterinary importance. Ticks were reported to have endosymbionts such as Coxiella-like, Francisella-like, Midichloria mitochondrii, Wolbachia, etc. With limited data on endosymbionts in ticks from India, we explored the endosymbiont diversity in ticks infesting the pets and livestock in Puducherry. Ectoparasites were collected from pet and domestic animals in 11 veterinary dispensaries between August to September 2022 and identified by taxonomical keys and sequencing of 16S rRNA, 18S rRNA, and Cytochrome oxidase subunit I genes. Wolbachia, Coxiella-like, Francisella-like and Midichloria mitochondrii endosymbionts were screened by PCR and their presence was confirmed by sequencing. A total of 984 ectoparasites including 5 tick, 3 lice, and 1 flea species were retrieved from 304 animals. Rhipicephalus sanguineus, Rhipicephalus haemaphysaloides, and Haemaphysalis bispinosa ticks were positive for the presence of Coxiella-like endosymbionts (CLEs) clustering under the clades B and C. Further, vertical transmission of CLEs in the field-collected R. sanguineus ticks was also demonstrated. This study offers an overview of the endosymbionts harbored by ticks in Puducherry with the first report of CLEs in H. bispinosa ticks and the transovarial transmission of endosymbionts in R. sanguineus ticks.

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Section: Discussionsupporting

confidence: 92%

supporting

confidence: 92%

Section: Discussioncontrasting

confidence: 42%

“…Rialch et al (2022) (15), demonstrated the transovarial transmission of endosymbionts in Rhipicephalus microplus ticks.…”

mentioning

confidence: 99%

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Detection and Genotyping of Coxiella-like Endosymbionts in Rhipicephalus and Haemaphysalis Ticks Infesting Pets and Livestock in Puducherry, India

Devaraju,

Gopal,

Arif

et al. 2024

J Bacteriol Virol.

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“…The percentage of positive samples in the current study was high in farms A (12.5%), D (11.7%) and C (10%), respectively. Elsewhere, other studies have used different PCR markers to detect Coxiella ‐like endosymbionts (CLE) in ticks collected from horses in South Korea (Seo et al., 2016 ) ticks collected from different animals (cattle, dogs and goats) and the environment in Zambia (Kobayashi et al., 2021 ) and from ticks collected from bovines in India (Rialch et al., 2022 ).…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of Coxiella burnetii and Coxiella species in rats and chickens from poultry farms in North West Province, South Africa

Ramatla,

Khumalo,

Matshotshi

et al. 2023

Veterinary Medicine & Sci

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BackgroundCoxiella burnetii is a bacterial pathogen that causes query fever and coxiellosis in humans and animals, respectively. There is a scarcity of studies on the prevalence of C. burnetii infections in rats and chickens in South Africa.ObjectiveThe aim of this study was to determine the occurrence of C. burnetii in rats and chickens sampled from poultry farms in the North West Province of South Africa.MethodsDNA was extracted from rodent kidneys (n = 68) and chicken faeces (n = 52). Two rodent pest species, namely Rattus rattus and Rattus tanezumi, were identified by analysis of CO1 gene sequences. Detection of C. burnetii was carried out using polymerase chain reaction assays targeting 23S rRNA, 16S rRNA and IS111 markers.ResultsC. burnetii was detected in 16.2%, 8.8% and 25% of R. rattus, R. tanezumi and chickens, respectively.ConclusionsThe findings in this study demonstrate that rodents and chickens are harbouring C. burnetii at sampled poultry farms. There should be frequent screening for C. burnetii in poultry operations. The likelihood of future transmission between rodents and chickens, including humans, also needs to be investigated.

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Molecular detection of Coxiella-like endosymbionts in Rhipicephalus microplus from north India

Cited by 2 publications

References 36 publications

Detection and Genotyping of Coxiella-like Endosymbionts in Rhipicephalus and Haemaphysalis Ticks Infesting Pets and Livestock in Puducherry, India

Detection and Genotyping of Coxiella-like Endosymbionts in Rhipicephalus and Haemaphysalis Ticks Infesting Pets and Livestock in Puducherry, India

Molecular detection of Coxiella burnetii and Coxiella species in rats and chickens from poultry farms in North West Province, South Africa

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