Molecular detection of Deformed Wing Virus (DWV) in honeybees from Ardahan province in Turkey

Gülmez, Yaşar; Can, İlyas; Teki̇n, Şaban

doi:10.1016/j.jbiotec.2012.07.097

Cited by 5 publications

(6 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Studies on the detection of viral pathogens that cause disease in honey bees have been carried out in our country for the last 15 years. Within the scope of these studies, Gülmez et al (2009) detected DWV for the first time in Türkiye as a result of their study on honey bees in Ordu province [13]. Muz and Muz (2009) identified DWV in Hatay province [43].…”

Section: Discussionmentioning

confidence: 98%

“…These two viruses are also very common in Turkish honey bees. DWV and BQCV viruses were detected for the first time in the Black Sea region in studies conducted in 2009-2010 in Türkiye [13,14]. Although viral diseases of honey bees grown in many localities of Türkiye were detected, there are no studies on diseases and pathogens of honey bees in Erzurum, which has honey forests in the Eastern Anatolia region and is one of the attraction centers of beekeeping.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Determination of Mixed Virus Infection in Honey Bees from Erzurum, Türkiye

SELVİTOPİ,

EROĞLU

2023

Türk Doğa Ve Fen Dergisi

View full text Add to dashboard Cite

Honey bees are the most important pollinators of agricultural products and provide pollination. Especially the worker bees, which make up the majority of the honey bee population, produce products with high economic value such as honey, pollen, propolis, royal jelly, bee venom, and beeswax. Mass deaths are observed in honey bees grown in Erzurum province, where beekeeping activities are carried out intensively in the Eastern Anatolia region of Türkiye. Epidemic diseases seen in honey bees in Erzurum have a very negative effect on the development and progress of beekeeping activities. Most of the diseases that cause epidemics in bees and cause sudden death and colony loss are of viral origin. In this study, viral pathogens that cause death in honey bees in Erzurum province were investigated and it was determined that two honeybee viruses, the black queen cell virus (BQCV), and the deformed wing virus, caused intense epidemics. In addition, phylogenetic analyzes revealed that all BQCV isolates found in this study clustered quite far from BQCV isolates previously isolated from Turkiye, while DWV isolates clustered close to Hakkari and Lithuania isolates.

show abstract

Section: Discussionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 99%

Determination of Mixed Virus Infection in Honey Bees from Erzurum, Türkiye

SELVİTOPİ,

EROĞLU

2023

Türk Doğa Ve Fen Dergisi

View full text Add to dashboard Cite

show abstract

“…Hence, it is necessary to conduct accurate and rapid diagnoses along with combating these diseases by proper protection measures in place. In this way, we can protect the health of honey bees and contribute to future generations and the ecosystem (17,20,31,36,44). Difficulties are encountered while assessing the epidemiological status of countries because it is not compulsory to report the DWV, ABPV, and BQCV infections in disease reporting systems such as Animal Disease Notification System (ADNS) and World Organization for Animal Health (OIE).…”

Section: Discussionmentioning

confidence: 99%

“…Many studies have been conducted in our country related to DWV. One study was conducted in the queen bee colonies, where approximately 50% of the hives were reported to be infected with this virus (17). Karapınar et al ( 21) detected a high rate of DWV disease, i.e., 69.23% in the province of Van, which was also similar to the prevalence of Varroa parasite in these colonies.…”

Section: And Tropilaelaps Mites (22)mentioning

confidence: 96%

Investigation of deformed wing virus, black queen cell virus, and acute bee paralysis virus infections in honey bees using reverse transcriptase-polymerase chain reaction (RT-PCR) method

Usta

Yıldırım

2022

Ankara Üniversitesi Veteriner Fakültesi Dergisi

View full text Add to dashboard Cite

Viruses are one of the most potential risk factors that negatively affect different life stages of honey bees. This study was conducted to determine the presence of infections caused by the deformed wing virus (DWV), black queen cell virus (BQCV), and acute bee paralysis virus (ABPV) in honey bees in the beekeeping regions of Burdur, along with obtaining information about their prevalence in this particular region. In our study, the adult bees were taken from 31 different beehives and comb samples that had different honey bee breeds and were sampled randomly from 15 beekeeping areas within the region. The collected samples were analyzed using the reverse transcriptase-polymerase chain reaction (RT-PCR), and the prevalence of DWV, BQCV, and ABPV infections were determined to be 74.19% (23/31), 25.81%, (8/31) and 74.19% (23/31), respectively. In this study, the distribution of positive samples and the rates of multiple infections were determined in the colonies. Of the positive honey bee samples, 12 (%38,71) were detected only for one virus, 9 (%29,03) were positive for two viruses (DWV-ABPV) and 8 (%25,81) were positive for all three viruses.In the present study, the presence of the three bee viruses that caused significant damage to the colonies by multiple infections in the Burdur region, was determined with RT-PCR. To our knowledge, this is the first report of three mentioned bee infections in honey bees in Burdur region. The revealed epidemiological conditions lead to the conclusion that serious measures are needed to control these infections in this region. Keywords: Acute bee paralysis virus, black queen cell virus, deformed wing virus, honey bee, RT-PCR. olarak saptanması ve bu yöredeki yaygınlıkları hakkında bilgi edinilmesi amacıyla yapılmıştır. Araştırmada söz konusu yörede bulunan 15 arıcılık işletmesinden tesadüfi örnekleme ile farklı arı ırklarından 31 farklı kovandan ergin arı ve yavrulu petek örneği usulüne uygun olarak alındı. Toplanan numuneler reverse transcriptase-polymerase chain reaction (RT-PCR) metodu ile analiz edildi ve DKV, BKHV, AAFV enfeksiyonlarının prevalansı sırasıyla %74,19 (23/31), %25,81 (8/31), %74,19 (23/31) oranlarında belirlendi. Çalışmanın yürütüldüğü kolonilerde tespit edilen pozitifliğin ırklara göre dağılımı ve çoklu enfeksiyon oranları tespit edildi. Pozitif örneklerin 12'si (%38,71) sadece bir virusa, 9'u (%29,03) iki virusa (DKV-AAFV) ve 8'ide (%25,81) üç virusa karşı pozitif bulundu. Bu çalışmada Burdur yöresinde çoklu enfeksiyonlarla kolonilercde önemli hasara neden olan üç arı virusunun varlığı RT-PCR ile belirlendi. Bu çalışma, Burdur bölgesindeki bal arılarında adı geçen üç virusun varlığına ilişkin bilinen ilk raporu sunmaktadır. Tespit edilen epidemiyolojik sonuçlara göre yörede bu enfeksiyonların kontrolü amacıyla ciddi önlemlerin alınması gerektiği kanaatine varıldı. Anahtar sözcükler: Akut arı felci virus, bal arısı, deforme kanat virus, siyah kraliçe hücre virus, RT-PCR.

show abstract

“…Some of the studies on the diagnosis of microbial diseases in honey bees in Turkey are as follows: Gülmez et al (2009) detected DWV for the first time in Turkey as a result of their study on honey bees in Ordu province. Muz and Muz (2009) Kalayci et al (2020) reported that the DWV pathogen was the most common in honey bee samples from Adana, Aydın, Bursa, Izmir, Kütahya, Muğla, and Manisa, while the CBPV pathogen was less common.…”

Section: Detection Of Microbial Pathogensmentioning

confidence: 99%

Molecular Identification of Microbial Pathogens in Honey Bees From Amasya

Utkan,

Eroğlu

2023

Uludağ Arıcılık Dergisi

View full text Add to dashboard Cite

Honey bees, Apis mellifera are highly beneficial insects that constitute both the livelihood of the producers and the food source of the consumers. However, there are some diseases that affect the yield of bees and cause the collapse of almost the entire colony. Most of these diseases are caused by microbial pathogens originating from viruses, bacteria, and fungi. Beekeeping is an important source of livelihood both in the center of Amasya and in almost all its districts. In this study, microbial pathogens that cause mass bee deaths and epidemics in Amasya province were determined using molecular methods. The results showed that the most common honey bee pathogens in Amasya are the Deformed wing virus, Chronic bee paralysis virus, and Aspergillus flavus fungus. Thus, the profile of bee diseases in Amasya province was determined for the first time with this study. In addition, this study guides other studies planned for the prevention of bee diseases and healthy beekeeping.

show abstract

Molecular detection of Deformed Wing Virus (DWV) in honeybees from Ardahan province in Turkey

Cited by 5 publications

References 0 publications

Determination of Mixed Virus Infection in Honey Bees from Erzurum, Türkiye

Determination of Mixed Virus Infection in Honey Bees from Erzurum, Türkiye

Investigation of deformed wing virus, black queen cell virus, and acute bee paralysis virus infections in honey bees using reverse transcriptase-polymerase chain reaction (RT-PCR) method

Molecular Identification of Microbial Pathogens in Honey Bees From Amasya

Contact Info

Product

Resources

About