Molecular detection of Dirofilaria immitis in dogs and mosquitoes in Tabasco, Mexico

Torres-Chablé, Oswaldo M.; Baak-Baak, Carlos M.; Cigarroa-Toledo, Nohemí; Blitvich, Bradley J.; Brito-Argaez, Ligia G; Alvarado-Kantun, Yessenia N; Zaragoza-Vera, Claudia Virginia; Arjona-Jimenez, Guadalupe; Moreno-Perez, Lluvia G.; Medina-Perez, Pablo; Machaín-Williams, Carlos; Garcia-Rejon, Julián E.

doi:10.4103/0972-9062.242563

Cited by 19 publications

(11 citation statements)

References 2 publications

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“…In one sample, only PCR4 was positive, thus suggesting single infection by A. reconditum. Torres-Chable et al (2018) pointed out that is important to assay for other filarial nematodes, including inconsequential pathogens, to minimalize the likelihood of misdiagnosis that commonly occurs when using the Knott's test if species differentiation is not considered.…”

Section: Discussionmentioning

confidence: 99%

Detection of Dirofilaria immitis using microscopic, serological and molecular techniques among dogs in Cabo Frio, RJ, Brazil

Trancoso

Lima

Barbosa

et al. 2020

Rev. Bras. Parasitol. Vet.

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Heartworm disease is a health problem for dogs and cats, especially in tropical and subtropical coastal regions of the world. Some studies have compared the efficacy of the diagnostic techniques used to detect this parasitosis. Therefore, the aim of this study was to compare parasitological optical microscopy (POM), serological and molecular techniques for diagnosing canine heartworm infection. Samples were collected between July 2015 and April 2016 from 103 dogs in Cabo Frio, RJ, Brazil. The wet fresh blood, thick smears, thin smears and modified Knott’s test were used to detect microfilariae. ELISA (Snap™ 4Dx ® IDEXX) was used to detect antigens and the polymerase chain reaction (PCR) was used to detect DNA and enable sequencing for species differentiation and confirmation. 19.4% of samples were positive according to microscopy. Through PCR, 15.5% of the total were positive. Using ELISA, the positivity rate was 29.1%. Occult heartworm infection was detected in 11.6% of the samples. ELISA sensitivity was shown to be higher than PCR or microscopy (P = 0.001). Sequencing of samples confirmed the presence of Dirofilaria immitis and Acanthocheilonema reconditum . ELISA was more effective for serological diagnosis canine heartworm and should be used in clinical and epidemiological studies.

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Section: Discussionmentioning

confidence: 99%

Detection of Dirofilaria immitis using microscopic, serological and molecular techniques among dogs in Cabo Frio, RJ, Brazil

Trancoso

Lima

Barbosa

et al. 2020

Rev. Bras. Parasitol. Vet.

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“…Resultados consistentes con este estudio fueron similares a los encontrados en los municipios de Bahía de Banderas Nayarit (12.7% prevalencia) (González-Morteo et al, 2015) y Chontalpa Tabasco (17.5% prevalencia) (Torres-Chable et al, 2018); pero diferentes a los reportados por Rodríguez-Vivas et al (1994) en el estado de Yucatán (6.54% prevalencia). Estas mismas investigaciones (Rodríguez-Vivas et al, 1994;González-Morteo et al, 2015;Torres-Chable et al, 2018), mencionan no haber encontrado efecto entre los sexos (machos y hembras), edad, talla, raza, lugar donde habitaban (dentro o fuera de la vivienda) y convivencia con otros caninos. Sin embargo, en el presente estudio los animales donde se encontró las microfilarias fueron machos, con alta CC (3 y 5 puntos), talla chica y mediana.…”

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Prevalencia de Dirofilaria immitis en caninos domésticos de dos municipios del trópico de Guerrero, México

et al. 2019

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“…Third one is mosquito vectors are not common in the sampling region due to the climatic conditions. Prevalence of D. immitis was determined as 21.3% in France [38], 14.5% in Tunisia [29], 13.7% in Portugal [10], 8.0% in Mexico [40], 2.3-4.02% in Iran [4,24], 1.5-3.7% in Turkey [3,13,37] and 0.0% in Cape Verde [20] with PCR. Environmental factors particularly climate and ecologic features influence mosquito population and this can be related to prevalence of dirofilariosis.…”

Section: Discussionmentioning

confidence: 99%

“…Dirofilaria immitis, a pathogenic filarial nematode in dogs, other canids and humans [19], cause cardiopulmonary problems (heartworm disease) in many geographic regions of Asia [14,22,27,37,41,43], Europe [9,10,12,30], Africa [29,39], Australia [8] and America [40].…”

Section: Introductionmentioning

confidence: 99%

First Molecular Detection of Dirofilaria immitis and D. repens in Dogs from Kyrgyzstan

et al. 2020

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Background Dirofilaria immitis and Dirofilaria repens are the causative agents of cardiopulmonary and subcutaneous dirofilariosis, respectively. This neglected disease mainly seen in dogs, cats and wild carnivores is re-emerging recent years. No study was conducted on dirofilariosis in dogs in Kyrgyzstan. Purpose The goal of this study was to investigate Dirofilaria species using PCR and sequencing in dogs from Kyrgyzstan. Method Dirofilaria spp. infection in dogs was screened via convential PCR and sequencing in 337 dogs from Kyrgyzstan. Result The overall prevalence of Dirofilaria spp. was 0.59% (2/337): DNA of D. immitis was detected in one sample and DNA of D. repens in second positive sample. In second sample, parallel co-infection of D. repens with Wolbachia was also found. While D. immitis sequence showed 98.70-100% similarity with previously reported sequences of D. immitis from dog blood, D. repens shared 100% identity with other sequences of D. repens. Conclusion These results provided first evidence for Dirofilaria spp. in Kyrgyzstan and emphasized the veterinary and medical importance.

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Molecular detection of Dirofilaria immitis in dogs and mosquitoes in Tabasco, Mexico

Cited by 19 publications

References 2 publications

Detection of Dirofilaria immitis using microscopic, serological and molecular techniques among dogs in Cabo Frio, RJ, Brazil

Detection of Dirofilaria immitis using microscopic, serological and molecular techniques among dogs in Cabo Frio, RJ, Brazil

Prevalencia de Dirofilaria immitis en caninos domésticos de dos municipios del trópico de Guerrero, México

First Molecular Detection of Dirofilaria immitis and D. repens in Dogs from Kyrgyzstan

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