Molecular detection of Ehrlichia canis in cats in Brazil

Oliveira, L.S. de; Mourão, Luíza Carvalho; Oliveira, Karla Andrade de; Agostini, Marcelo; Oliveira, Alice Carvalho de; Almeida, Márcia Rogéria de; Fietto, Juliana Lopes Rangel; Conceição, Lissandro Gonçalves; Filho, José Dantas Ribeiro; Galvão, Márcio Antônio Moreira; Mafra, Cláudio

doi:10.1111/j.1469-0691.2008.02175.x

Cited by 32 publications

(31 citation statements)

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“…The PCR results revealed that positive cats have a tendency for lymphopenia (p= 0.07), a finding that is commonly reported in animals in the acute phase of infection (HARRUS et al, 1997). The evaluation of the positive cats indicated that most of them presented thrombocytopenia, which is consistent with the findings of Oliveira et al (2009). However, these results were not statistically confirmed.…”

Section: Discussioncontrasting

confidence: 47%

“…The 16S rRNA gene was amplified by two-step PCR with the primers ECC (5'-AGA ACG AAC GCT GGC GGC AAG CC -3') and ECB (5'-CGT ATT ACC GCG GCT GCT GGC-3') in the first reaction at an annealing temperature of 65 °C, yielding an amplicon of 458 pb, and with the primers ECAN (5'-CAA TTA TTT ATA GCC TCT GGC TAT AGGA -3') and HE3 (5'-TAT AGG TAC CGT CAT TAT CTT CCC TAT -3') in the second reaction at an annealing temperature of 55 °C, yielding an amplicon of 398 pb (OLIVEIRA et al, 2009). A PCR negative control (ultrapure -MilliQ®) and positive control (DNA from the São Paulo strain of E. canis obtained from DH82 culture) were used in each reaction.…”

Section: Nucleic Acid Extraction and Pcrmentioning

confidence: 99%

“…Symptoms suggestive of ehrlichiosis infection have also been reported in domestic cats in several countries. The Ehrlichia species that naturally infect cats have not yet been fully determined, although monocyte and lymphocyte inclusions and, more consistently, E. canis DNA, have been detected in cats (BREITSCHWERDT et al, 2002;OLIVEIRA et al, 2009;BRAGA et al, 2012), as well as some granulocytic inclusions related to feline granulocytic ehrlichiosis caused by Anaplasma phagocytophilum (BUORO et al, 1989;BOULOY et al, 1994;BEAUFILS et al, 1995;BJOERSDORFF et al, 1999;SHAW et al, 2001;AGUIRRE et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

“…Some reports of E. canis infection in cats have shown hematological alterations such as bone marrow hypoplasia or dysplasia, accompanied by pancytopenia or anemia and thrombocytopenia. Based on clinical and laboratory findings, the pathogenesis of the disease seems likely to be similar to Canine Monocytic Ehrlichiosis (CME) (BREITSCHWERDT et al, 2002;OLIVEIRA et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

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Hematological values associated to the serological and molecular diagnostic in cats suspected of Ehrlichia canisinfection

Braga

Santos

Melo

et al. 2013

Rev. Bras. Parasitol. Vet.

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The literature contains several studies on feline ehrlichiosis. However, information about the characteristics of Ehrlichia infection in cats is still scanty. This study evaluated the association between Ehrlichia spp. infection and the hematologic data of 93 cats treated at the Federal University of Mato Grosso Veterinary Hospital in Cuiabá, state of Mato Grosso, Brazil. The presence of or exposure to Ehrlichia spp. infection was evaluated by Polymerase Chain Reaction (PCR) targeting the dsb and 16S rRNA gene of Ehrlichia, and by detection of anti-Ehrlichia canis IgG antibodies in Indirect Fluorescence Assay (IFA), respectively. Eight (8.6%) cats tested positive by PCR and the partial DNA sequence obtained from PCR products was a 100% match to E. canis. Forty-two (45.1%) cats showed antibody reactivity against Ehrlichia spp. Hematological alterations such as low erythrocyte count, thrombocytopenia, lymphopenia and monocytosis were observed in PCR positive cats. Among them, low erythrocyte counts were associated with IgG antibody titers of 40 to 640 and five cats also tested positive by PCR. Furthermore, PCR-positive cats showed a tendency to be lymphopenic. No correlation was found between age and sex, and no ticks were observed in any of the examined cats.Keywords: Ehrlichiosis, feline, hematology, polymerase chain reaction, indirect fluorescence assay, diagnosis. ResumoDiversos estudos sobre erliquiose felina vêm sendo relatados na literatura. No entanto, a caracterização da infecção por Ehrlichia em gatos ainda é escassa. O presente estudo objetivou avaliar a associação entre infecção por Ehrlichia e dados hematológicos em 93 gatos atendidos no Hospital Veterinário da Universidade Federal de Mato Grosso, em Cuiabá, Brasil. A presença de infecção por Ehrlichia spp. foi avaliada pela Reação em Cadeia pela Polimerase (PCR) visando à amplificação dos genes dsb e 16S rRNA de Ehrlichia e por Reação de Imunofluorescência Indireta (RIFI). Oito (8,6%) gatos demonstraram ser positivos pela PCR, sendo suas sequências parciais de DNA 100% idênticas à E. canis. Quarenta e dois gatos (45,1%) apresentaram anticorpos reativos contra Ehrlichia spp. Alterações hematológicas como baixas contagens de eritrócitos, trombocitopenia, linfopenia e monocitose foram observadas em gatos positivos pela PCR. Dentre essas, eritropenia foi associada em gatos com títulos de anticorpos IgG entre 40 e 640, sendo cinco destes positivos pela PCR. Adicionalmente, gatos positivos na PCR apresentaram uma tendência a serem linfopênicos. Não foram observadas associações entre a presença de infecção nos gatos e suas respectivas idades e sexo. Nenhum carrapato foi observado nos gatos examinados.Palavras-chave: Erliquiose, felinos, hematologia, reação em cadeia pela Polimerase, reação de imunofluorescência indireta, diagnóstico.

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Section: Discussioncontrasting

confidence: 47%

Section: Nucleic Acid Extraction and Pcrmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hematological values associated to the serological and molecular diagnostic in cats suspected of Ehrlichia canisinfection

Braga

Santos

Melo

et al. 2013

Rev. Bras. Parasitol. Vet.

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“…Ehrlichia organisms have not been cultured from feline samples; evidence of infection came exclusively from serological and molecular studies (BREITSCHWERDT et al, 2002;OLIVEIRA et al, 2009b). Nevertheless, Ehrlichia canis-like organisms were detected by PCR and DNA sequencing in cats from Brazil and North America (BREITSCHWERDT et al, 2002;OLIVEIRA et al, 2009b). The first molecular detection of E. canis in Brazilian cats was reported in a study at a VTH of Minas Gerais State, Southeastern Brazil, in which blood samples from 3/15 cats tested positive by nested-PCR for the 16S rRNA gene.…”

Section: Ehrlichiosis In Catsmentioning

confidence: 99%

Ehrlichiosis in Brazil

Vieira

Biondo

Guimarães

et al. 2011

Rev. Bras. Parasitol. Vet.

123

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Ehrlichiosis is a disease caused by rickettsial organisms belonging to the genus Ehrlichia. In Brazil, molecular and serological studies have evaluated the occurrence of Ehrlichia species in dogs, cats, wild animals and humans. Ehrlichia canis is the main species found in dogs in Brazil, although E. ewingii infection has been recently suspected in five dogs. Ehrlichia chaffeensis DNA has been detected and characterized in mash deer, whereas E. muris and E. ruminantium have not yet been identified in Brazil. Canine monocytic ehrlichiosis caused by E. canis appears to be highly endemic in several regions of Brazil, however prevalence data are not available for several regions. Ehrlichia canis DNA also has been detected and molecularly characterized in three domestic cats, and antibodies against E. canis were detected in free-ranging Neotropical felids. There is serological evidence suggesting the occurrence of human ehrlichiosis in Brazil but its etiologic agent has not yet been established. Improved molecular diagnostic resources for laboratory testing will allow better identification and characterization of ehrlichial organisms associated with human ehrlichiosis in Brazil.

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