Molecular Detection of Ehrlichia canis in Dogs in Malaysia

Nazari, Mojgan; Lim, Sue Yee; Sharma, Reuben Sunil Kumar; Cheng, Nadzariah A. B. Y.; Watanabe, Malaika

doi:10.1371/journal.pntd.0001982

Cited by 48 publications

(31 citation statements)

References 21 publications

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“…The detection of E. canis DNA in 22.9% of the sampled population of dogs was higher than the prevalence of 12.7% reported by Kamani et al (2013) in the northern part of Nigeria and those reported by Nazari et al (2013) and Lasta et al (2013) in Malaysia and Brazil, respectively. The higher occurrence reported in this study may partly be associated with the predominance of the tick vector (Rhipicephalus sanguineus) of the parasite in the study area (Oke et al, 2013) and may be due to optimum environmental conditions that favour its breeding.…”

Section: Discussioncontrasting

confidence: 63%

“…The prevalence and molecular characteristics of Ehrlichia canis have been studied extensively around the world (Suksawat et al, 2001;Unver et al, 2001;Vinasco et al, 2007;Aguiar et al, 2008;Harrus et al, 2011;Nazari et al, 2013;Milanjeet et al, 2014;Cardoso et al, 2016). One of these studies has revealed that more than one strain may exist in a region (Vinasco et al, 2007).…”

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confidence: 99%

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Detection and molecular characterisation of Ehrlichia canis in naturally infected dogs in South West Nigeria

Daramola

Takeet

Oyewusi

et al. 2018

Acta Veterinaria Hungarica

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Canine ehrlichiosis is an important tick-borne rickettsial disease mainly caused by Ehrlichia canis. This study aimed to detect and characterise E. canis in dogs in Abeokuta, Nigeria by microscopy and nested PCR. Blood samples were collected from 205 dogs, thin smears were made, field-stained, and DNA was extracted from the blood samples. A partial region of the 16S rRNA gene was amplified by polymerase chain reaction (PCR) and sequenced unidirectionally. Ehrlichial morulae were detected in three dogs (1.5%). The PCR test revealed that 47 dogs (22.9%) were positive for E. canis. The lengths of the sequences obtained range from 374 bp to 376 bp with an average G-C content of 37% and 98-99% homology with the reference sequences in GenBank. The aligned autochthonous sequences were less polymorphic. The phylogenetic analysis separated sequences reported previously in Nigeria from the autochthonous sequences. The present work shows that the strain of E. canis detected in the study area is genetically different from those reported in the northern part of Nigeria and more closely related to sequences from Brazil and India.

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Section: Discussioncontrasting

confidence: 63%

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confidence: 99%

Detection and molecular characterisation of Ehrlichia canis in naturally infected dogs in South West Nigeria

Daramola

Takeet

Oyewusi

et al. 2018

Acta Veterinaria Hungarica

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“…canis has been detected and reported in dogs from many parts of the world Rani et al 2011;Sasaki et al 2012;Ybañez et al 2012;Nazari et al 2013;Aktas et al 2015;Inpankaew et al 2016). An analysis of our results revealed a 28% overall prevalence of E. canis (N = 42) in the studied blood samples from the three districts, suggesting that E. canis is prevalent in Punjab province (Table 1) but the prevalence of E. canis varied significantly between three sampling areas indicating that geographical features and climatic conditions do affect the parasite prevalence (Rani et al 2011;Mircean et al 2012).…”

Section: Discussionmentioning

confidence: 99%

Molecular detection of Ehrlichia canis in dogs from three districts in Punjab (Pakistan)

Malik

Qamar

Ain

et al. 2018

Veterinary Medicine & Sci

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Canine monocytic ehrlichiosis is a tick-borne disease caused by an intracellular alpha-proteobacterium, Ehrlichia canis, which replicates within mononuclear cells in the host. This study was designed to use a polymerase chain reaction (PCR) protocol for the molecular detection of E. canis by the amplification of a portion of its 16S rRNA gene, as well as the effects of this alpha-proteobacterium on the haematological parameters of the sampled dogs and the risk factors associated with E. canis infection. A total of 151 blood samples were collected from dogs of various breeds at three sampling sites (Lahore, Rawalpindi/Islamabad and Multan) in Punjab, Pakistan. Data regarding the epidemiological factors (including age, gender, breed, body temperature, deworming, vaccination, mucous membrane status, hydration status, the presence of haematuria and tick infestation) were collected through a questionnaire at the time of sample collection. A 400 bp DNA fragment of the 16S rRNA gene of E. canis was amplified from 42 dog blood samples (28% of the total), [Lahore (N = 24), Rawalpindi/Islamabad (N = 13) and Multan (N = 05)] through PCR. Data analysis revealed that the character of the animals (age, sex and breed) had no significant association (P > 0.05) with the presence of E. canis. Various haematological parameters were also compared, and the results revealed that all of the parameters remained unaffected, except significantly lower white blood cell counts (P = 0.004) in E. canis-positive blood samples, as compared with the control group. We concluded that this is the first molecular confirmation of canine infection by E. canis using PCR. Moreover, no specific epidemiological parameter was found associated with the prevalence of E. canis in dogs.

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“…Studies in countries situated in and around the Middle Eastreport the presence of vector-borne pathogens including Anaplasma platys, Ehrlichia canis, Bartonella spp.,and Babesia spp. in dogs and cats [9,13,14,15,16,17,18,19].Currently, limited studies have examined the presence of vector-borne disease in dogs and cats in Saudi Arabia [7,20,21,22,23]. Therefore, the aim of this study was to evaluate the presence of vector-borne pathogens in dogs and cats in two different climatic zones of Saudi Arabia using a commercial diagnostic MT-PCR panel for the detection of Babesia gibsoni, Babesia vogeli, Mycoplasma haematoparvum, Mycoplasma haemocanis, Mycoplasma haemofelis, Anaplasma platys and Bartonella species.…”

Section: Introductionmentioning

confidence: 99%

Application of advanced molecular techniques to detect vector borne pathogens from stray dogs and cats in two different climatic zones of Saudi Arabia

Alanazi

Šlapeta

Alouffi

et al. 2020

Preprint

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Background: Vector-borne diseases have been increasing worldwide and reported in many animals including dogs and cats. Limited or no data are currently available regarding canine and feline vector-borne diseases in Saudi Arabia and limited information is available from other Middle Eastern countries. The aim of this study was to compare vector-borne disease prevalence between two bio-climatically distinct regions of Saudi Arabia, Riyadh province that is arid positioned at low elevation and Asir province that is humid at high elevation. Methods: Blood samples from 74d ogs from Riyadh province and 70 dogs and 44 cats from Asirprovince were collected and examined for the presence of genomic DNA of Babesias pp, Anaplasma spp., Ehrlichias pp., Bartonella spp., Mycoplasma spp., and Hepatozoon spp. by polymerase chain reaction (PCR), Multiplex-tandem PCR (MT-PCR) and Sanger sequencing.Results: Seventy four dogs were tested from Riyadh province and found be negative of any pathogen. Of the 70 dogs examined from Asir province 45(64.3%) were positive. Specifically, 40 (57.1%) dogs were positive for A.platys, 20 (28.5%) for B.vogeli, 11(15.7%) for My.Haemocanis, two (2.85%) for Candidatus Mycoplasma haematoparvum and one (1.4%) for Br.henselae. Fourteen out of 44 cats (31.8%) were positive for one of the detected vector-borne pathogens. Six cats (13.6%) were positive for Candidatus Mycoplasma haemominutum and My.haemofelis, respectively, four cats (9.2%) were positive for Br.Henselae, two (4.54%) for Candidatus Mycoplasma haematoparvum and one (2.27%) for A. platys. Conclusions: The results of this study report the occurrence of A. platys, B. vogeli, Br. henselae, and My. haemocanis in dogs and of A. platys, Br. henselae, My.haemofelis and Candidatus Mycoplasma haemominutum in cats from Asir province Further molecular investigations are strongly recommended in order to reduce the risk of dogs and cats acquiring vector-borne diseases in Saudi Arabia.

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Molecular Detection of Ehrlichia canis in Dogs in Malaysia

Cited by 48 publications

References 21 publications

Detection and molecular characterisation of Ehrlichia canis in naturally infected dogs in South West Nigeria

Detection and molecular characterisation of Ehrlichia canis in naturally infected dogs in South West Nigeria

Molecular detection of Ehrlichia canis in dogs from three districts in Punjab (Pakistan)

Application of advanced molecular techniques to detect vector borne pathogens from stray dogs and cats in two different climatic zones of Saudi Arabia

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