Molecular detection of Erwinia psidii in guava plants under greenhouse and field conditions

Silva, Claudênia Ferreira da; Uesugi, Carlos Hidemi; Blum, Luiz Eduardo Bassay; Marques, Ana Maria Campos; Ferreira, Marisa Álvares da Silva Velloso

doi:10.1590/0103-8478cr20151600

Cited by 8 publications

(9 citation statements)

References 16 publications

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“…juglandis -specific primer. DNA quantities are expected to be low in the wood shaving samples because the DNA would have come from molted exoskeletal structures or frass from the beetles [ 26 ]. Another possible explanation for the low detection rate of P .…”

Section: Discussionmentioning

confidence: 99%

“…Molecular techniques can provide valuable tools in plant disease diagnosis and can be used for the simultaneous detection and identification of plant pathogens [ 26 ]. PCR-based methods have become the primary means for the early detection of pathogens in agriculture and forestry [ 27 ], as well as in human and animal health [ 28 ].…”

Section: Introductionmentioning

confidence: 99%

“…PCR-based methods have become the primary means for the early detection of pathogens in agriculture and forestry [ 27 ], as well as in human and animal health [ 28 ]. Molecular methods are sensitive enough to detect minute amounts of DNA from the target organism even when the substrate is heterogeneous, thereby decreasing the time needed to isolate and confirm the presence of the pathogen of interest [ 26 , 27 , 29 , 30 ]. With the goal of reducing the time invested in the detection of both organisms involved in TCD, this study developed a protocol that utilizes species-specific microsatellite loci as molecular tools for their rapid detection.…”

Section: Introductionmentioning

confidence: 99%

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A novel molecular toolkit for rapid detection of the pathogen and primary vector of thousand cankers disease

et al. 2018

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Thousand Cankers Disease (TCD) of Juglans and Pterocarya (Juglandaceae) involves a fungal pathogen, Geosmithia morbida, and a primary insect vector, Pityophthorus juglandis. TCD was described originally from dying Juglans nigra trees in the western United States (USA), but it was reported subsequently from the eastern USA and northern Italy. The disease is often difficult to diagnose due to the absence of symptoms or signs on the bark surface of the host. Furthermore, disease symptoms can be confused with those caused by other biotic and abiotic agents. Thus, there is a critical need for a method for rapid detection of the pathogen and vector of TCD. Using species-specific microsatellite DNA markers, we developed a molecular protocol for the detection of G. morbida and P. juglandis. To demonstrate the utility of the method for delineating TCD quarantine zones, we tested whether geographical occurrence of symptoms and signs of TCD was correlated with molecular evidence for the presence of the cryptic TCD organisms. A total of 1600 drill cores were taken from branch sections collected from three regions (n = 40 trees for each location): California-J. hindsii (heavy disease incidence); Tennessee-J. nigra (mild disease incidence); and outside the known TCD zone (Missouri-J. nigra, no record of the disease). California samples had the highest incidence of the TCD organisms (85%, 34/40). Tennessee had intermediate incidence (42.5%, 17/40), whereas neither organism was detected in samples from Missouri. The low cost molecular protocol developed here has a high degree of sensitivity and specificity, and it significantly reduces sample-processing time, making the protocol a powerful tool for rapid detection of TCD.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A novel molecular toolkit for rapid detection of the pathogen and primary vector of thousand cankers disease

et al. 2018

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“…E. psidii can be disseminated by asymptomatic propagating plant material. Development of efficient methods to detect the pathogen in symptomatic and asymptomatic plant material has been the subject of several studies (Teixeira et al 2008;Silva et al 2015;Silva et al 2016). Bio-PCR, a variation of PCR that employs an enrichment step prior to DNA amplification (Schaad et al 1995) has been shown to be suitable for early disease diagnosis in guava orchards (Silva et al 2016).…”

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confidence: 99%

“…Several studies have focused on developing methods for inoculation on guava and eucalyptus (Marques et al 2007;Ferraz et al 2016), methods for disease diagnosis (Teixeira et al 2008;Silva et al 2015;Silva et al 2016), and assessment of pathogen diversity (Teixeira et al 2009). However, very Section Editor: Rodrigo Almeida Electronic supplementary material The online version of this article (https://doi.org/10.1007/s40858-018-0236-y) contains supplementary material, which is available to authorized users.…”

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confidence: 99%

Movement of the bacterial blight pathogen Erwinia psidii in guava varieties differing in susceptibility

Macagnan

Ferreira

2018

Trop. plant pathol.

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Erwinia psidii causes bacterial blight of guava, an important disease of this fruit crop in Brazil. Pathogen spread is associated with the movement of infected but asymptomatic propagating plant material. The objective of this study was to compare bacterial movement inside the host tissue and symptom development in two guava varieties commonly grown in Brazil. Guava plants of Pedro Sato and Kumagai varieties were inoculated with strain IBSBF435 by deposition of a bacterial suspension droplet followed by puncture at the axils of the first fully expanded pair of leaves. Disease severity was assessed in the upper third part of the plant and stem fragments were cut at intervals of 1 cm above and below the inoculation point. Samples were collected at 0.5, 1, 2, 4, 6, 8, 10 and 12 days after inoculation. Presence of viable cells of E. psidii at each sampling point was evaluated using Bio-PCR with species-specific primers after a 24 h-enrichment step. Bacterial movement was detected on both directions in both varieties, however a positive correlation between movement and disease severity was observed only for Pedro Sato. Kumagai plants remained symptomless despite the presence of E. psidii above and below the inoculation site.

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Draft Genomes of Six Philippine Erwinia mallotivora Isolates: Comparative Genomics and Genome-Wide Analysis of Candidate Secreted Proteins

Waje

Lantican

Pathania³

et al. 2022

Curr Microbiol

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Molecular detection of Erwinia psidii in guava plants under greenhouse and field conditions

Abstract: Erwinia psidii causes bacterial blight of guava (Psidium guajava), an important disease of this crop in Brazil. The pathogen affects branches and twigs of guava trees, reducing yield significantly. Bacterial dissemination often occurs through

Cited by 8 publications

References 16 publications

A novel molecular toolkit for rapid detection of the pathogen and primary vector of thousand cankers disease

A novel molecular toolkit for rapid detection of the pathogen and primary vector of thousand cankers disease

Movement of the bacterial blight pathogen Erwinia psidii in guava varieties differing in susceptibility

Draft Genomes of Six Philippine Erwinia mallotivora Isolates: Comparative Genomics and Genome-Wide Analysis of Candidate Secreted Proteins

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