Molecular detection of Fasciola, Schistosoma and Paramphistomum species from freshwater snails occurring in Gauteng and Free State provinces, South Africa

Molaba, Gantshe G.; Molefe-Nyembe, Nthatisi I.; Taioe, Oriel M.; Mofokeng, Lehlohonolo S.; Thekisoe, Oriel M.M.; Mtshali, Khethiwe

doi:10.1016/j.vetpar.2023.109978

Cited by 1 publication

(1 citation statement)

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“…Although some recent studies still utilize shedding of cercariae as sole detection method of infection [21], most recent studies couple the use of shedding cercariae with molecular techniques such as Restriction Fragment Length Polymorphism (RFLP) of Polymerase Chain Reaction (PCR) products [29] or sequencing of the PCR products for identification up to the species level [6,12,29]. Some studies also amplified the larval DNA directly from the snails, followed by sequencing [12,30] or RFLP of the products [29]. However, Schols [30] showed that detection of larval infection using PCR was better than cercariae shedding.…”

Section: Introductionmentioning

confidence: 99%

Amphistome Infection and Species Diversity of Freshwater Snails Collected from Selected Wildlife Drinking Water Sources in Matebeleland Region of Zimbabwe

Sibula,

Malatji,

Nyahunda

et al. 2024

Veterinary Sciences

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This study aimed at determining the identity of freshwater snails collected from selected water habitats frequented by wildlife as source of drinking water in the Matebeleland region of Zimbabwe and further screening the identified snails for natural infections with amphistomes using PCR. A total of 487 freshwater snails were collected from six areas in the Matebeleland region of Zimbabwe for identification and screening of amphistome infection. Eight freshwater snail species were morphologically identified and Biomphalaria pfeifferi, Bul. tropicus, Bul. truncatus, Bul. globosus, and L. (R.) natalensis were confirmed using the COI gene. Bulinus tropicus and Phy. acuta were the most abundant species at 33.9% (165/487) and 31.2% (155/487), respectively. DNA of amphistome was detected in 11.9% (58/487) of the collected snails. The highest infection rate was detected in Bul. globosus (44.4%). West Nicholson recorded the highest infection rate (33.9%), and infection was not detected in L. (R.) natalensis, Phy. acuta, and Bellamya spp. Amphistome DNA from M. tuberculata was successfully sequenced and identified as Calicophoron microbothrium. An additional band was detected in M. tuberculata, Bul. tropicus, and Bul. trancatus, which showed a 96.42% similarity to Paragonimus sp. sequence in the GenBank.

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