Molecular detection of hemoplasma infection among cats from São Luís island, Maranhão, Brazil

Braga, M.S.C.O.; André, Marcos Rogério; Freschi, Carla Roberta; Teixeira, Márcia Cristina Aquino; Machado, Rosangela Zacarias

doi:10.1590/s1517-83822012000200018

Cited by 16 publications

(26 citation statements)

References 36 publications

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“…Although hemoplasma infection has been documented in cats in several regions of Brazil (DE MORAIS et al, 2007;MACIEIRA et al, 2008;SANTOS et al, 2009;BRAGA et al, 2012;DE BORTOLI et al, 2012;MICELI et al, 2013), few reports clarifying actual epidemiology have yet been published and São Paulo (ANDRÉ et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…Previous studies conducted in Brazil have focused on different cat populations, such as animals maintained in animal shelters MICELI et al, 2013), domiciled cats with outdoor access (BRAGA et al, 2012), cats subjected to a spaying/neutering program (DE BORTOLI et al, 2012) and cats attended at veterinary clinics (MACIEIRA et al, 2008). The molecular occurrences of M. haemofelis (10.5%), 'Candidatus M. haemominutum' (12.8%) and 'Candidatus M. turicensis' (10.5%) found in the present study among domiciled cats subjected to a spaying/neutering program in the CCZ of Campo Grande were higher than those found by de Bortoli et al (2012) (2.2%; 4.3%; 2.2%), who sampled a similar population of cats in the city of Jaboticabal, state of São Paulo.…”

Section: Discussionmentioning

confidence: 99%

“…Samples that were positive for M. haemofelis and 'Candidatus M. haemominutum' were subjected to another PCR protocol (CRIADO-FORNELIO et al, 2003), in which primers amplify a fragment of 600 bp. Mycoplasma haemofelis, 'Candidatus M. haemominutum' and 'Candidatus M. turicensis' DNA obtained from blood samples from naturally infected cats in São Luis, Maranhão, Brazil (BRAGA et al, 2012) were used as positive DNA controls in PCR reactions for hemoplasmas. The reaction products were purified using the Silica Bead DNA gel extraction kit (Fermentas, São Paulo, SP, Brazil).…”

Section: Methodsmentioning

confidence: 99%

“…Hemoplasma infections among cats have been reported in some areas in Brazil over the last decade (MACIEIRA et al, 2008;DE BORTOLI et al, 2012;BRAGA et al, 2012;MICELI et al, 2013). Because of the potential risk to human health (SANTOS et al, 2008), the present study aimed to clarify the epidemiology of hemoplasmas in domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul (MS), Brazil.…”

Section: Introductionmentioning

confidence: 97%

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Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil

Santis

Herrera

Sousa

et al. 2014

Rev. Bras. Parasitol. Vet.

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Hemoplasmas are bacteria living in feline red blood cells. Feline hemoplasmosis is frequently associated with old male cats that have access to the streets. This study aimed to detect the presence of hemoplasma speciess in domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul (MS), Brazil, using molecular techniques. Between January 2013 and April 2013, EDTA-whole blood samples were collected from 151 domestic cats (65 free-roaming and 86 domiciled cats). Samples were subjected to PCR assays targeting hemoplasmas 16S rRNA, followed by sequencing, BLAST analysis and phylogenetic analysis. Results show an occurrence of 36.4% for hemoplasmas. Twenty-three cats (15.2%) were positive for 'Candidatus Mycoplasma haemominutum', 17 (11.2%) for M. haemofelis and 15 (9.9%) for 'Candidatus M. turicensis', from PCR. Coinfection by two or three hemoplasmas was found in 25 cats (16.6%). No statistically significant difference between genders or between lifestyles was observed for the presence of hemoplasmas among the cats. Results show different hemoplasma species are present in cat population (Campo Grande, MS, Brazil). It is suggested that a differential diagnosis for feline hemoplasmosis should be made when cats show nonspecific clinical signs of disease with systemic manifestation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 97%

See 2 more Smart Citations

Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil

Santis

Herrera

Sousa

et al. 2014

Rev. Bras. Parasitol. Vet.

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“…A parasitemia é intermitente e os micoplasmas nem sempre são identificados no esfregaço sanguíneo. O diagnóstico é feito através do histórico, sinais clínicos apresentados e do exame parasitológico através de ensaios baseados na PCR (BRAGA et al, 2012 (BIONDO et al, 2009;ANDRÉ et al, 2011).…”

Section: Introductionunclassified

Mycoplasma haemofelis em gato - relato de caso

Silveira¹,

Pimentel²,

Marques³

2015

Pubvet

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ResumoMycoplasma haemofelis é uma bactéria pleomórfica que parasita as hemácias de várias espécies domésticas e de potencial zoonótico. O presente relato apresenta o caso de um felino, fêmea, não castrada, dois anos de idade, da raça persa com histórico de anorexia, leve desidratação, prostração, escore corporal entre 1 e 2, letargia, linfonodos mandibulares e poplíteos aumentados de tamanho, mucosas levemente hipocoradas, TR 40.3ºC, urina concentrada e esplenomegalia moderada. O resultado da avaliação hematológica, bioquímica e o exame de PCR apontaram para o caso de micoplasmose felina. A

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Occurrence and molecular characterization of hemoplasmas in domestic dogs and wild mammals in a Brazilian wetland

et al. 2017

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Hemotropic mycoplasmas are known to cause anemia in several mammalian species. The present work aimed to investigate the occurrence of Mycoplasma spp. in wild mammals, domestic dogs and their respective ectoparasites, in southern Pantanal region, central-western Brazil. Between August 2013 and March 2015, 31 Nasua nasua, 78 Cerdocyon thous, seven Leopardus pardalis, 42 dogs, 110 wild rodents, and 30 marsupials were trapped and ectoparasites (ticks and fleas) found parasitizing the animals were collected. Mammals and ectoparasites DNA samples were submitted to conventional PCR assays for Mycoplasma spp. targeting 16S rRNA and RnaseP genes. Twenty-four N. nasua, three C. thous, two domestic dogs, one L. pardalis and one wild rodent were positive for 16S rRNA PCR protocols. Fourteen N. nasua samples were also positive in RnaseP PCR. No marsupial or arthropod showed positivity for Mycoplasma spp. The phylogenetic analyses based on 16S rRNA gene showed that all sequences obtained from dogs, two sequences obtained from C. thous and ten sequences obtained from N. nasua showed to be closely related to Mycoplasma haemocanis/Mycoplasma haemofelis species. Genotypes closely related to 'Candidatus Mycoplasma haemominutum' and Mycoplasma haemomuris were detected in the L. pardalis and in the wild rodent, respectively. Probably a novel Mycoplasma genotype, closely related to a sequence obtained from a Brazilian capybara was detected in 14 N. nasua, based on a concatenated phylogenetic analysis of 16S rRNA and RnaseP genes. The present study revealed that wild animals in southern Pantanal region, Brazil, are exposed to different species of hemoplasmas.

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Molecular detection of hemoplasma infection among cats from São Luís island, Maranhão, Brazil

Cited by 16 publications

References 36 publications

Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil

Molecular detection of hemotrophic mycoplasmas among domiciled and free-roaming cats in Campo Grande, state of Mato Grosso do Sul, Brazil

Mycoplasma haemofelis em gato - relato de caso

Occurrence and molecular characterization of hemoplasmas in domestic dogs and wild mammals in a Brazilian wetland

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