Molecular Detection of Human Papillomavirus DNA in Oral Lichen Planus Patients

Sameera, Ayesha; Kotikalpudi, Rosaiah; Patel, Rajesh K; Reddy, Kranti Kiran; Prasanna,; Erugula, Sridhar Reddy

doi:10.7860/jcdr/2019/32397.12466

Cited by 9 publications

(21 citation statements)

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“…The most frequent clinical manifestation is a reticulated pattern of white striae. [ 16 ] However, in our current investigation, we discovered a higher prevalence of erosive lesions. HPV infection rates in OLP have been reported at rates ranging from 15.4% to 42.6%.…”

Section: Discussioncontrasting

confidence: 62%

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PCR-based Evaluation of Human Papillomavirus Genotypes in Oral Lichen Planus

Vijayan

Muthukrishnan

Nair

et al. 2022

Journal of Pharmacy and Bioallied Sciences

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Objective: The objective of the study was to use polymerase chain reaction (PCR) to examine and compare the genotype distribution of human papillomavirus (HPV) in oral lichen planus (OLP). Materials and Methods: Deoxyribonucleic acid (DNA) was extracted from 20 OLP biopsy specimens. Conventional PCR assay employing consensus HPV primers was used to identify HPV DNA. Positive PCR samples were then subjected to PCR assay with HPV type-specific primers. Results: Out of the total 20 OLP specimens evaluated, eight samples (40%) were positive for HPV. Females had a 41.7% higher HPV-positive rate than males. The most common type in the HPV type-specific PCR assay was HPV-18 (75%), which is a high-risk type of HPV linked to malignant diseases. The erosive kind of OLP had the greatest percentage of HPV positives (50%). Conclusion: The present study confirms the detection of HPV in OLP lesions, as determined by PCR-coupled HPV gene sequencing, as well as its likely mechanism of malignant transformation.

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Section: Discussioncontrasting

confidence: 62%

“…[ 14 ] However, the current study found a greater positivity rate of HPV-18 genome, which was in line with a similar previous study. [ 16 ]…”

Section: Discussionmentioning

confidence: 99%

PCR-based Evaluation of Human Papillomavirus Genotypes in Oral Lichen Planus

Vijayan

Muthukrishnan

Nair

et al. 2022

Journal of Pharmacy and Bioallied Sciences

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“…It is these genotypic differences that permit stratification of the virus oncogenic phenotype into high and low-risk types. High risk includes HPV- 16,18,31,33,35,45,51,52,56,58,59 and low risk are HPV 6,11,42,43,44.The molecular mechanisms by which HPVs disrupt key cellular elements responsible for cell cycle regulation and apoptosis have been identified. [33,34] Degradation of p53 by the E6 protein of HR-HPV is also one of the mechanisms by which the normal function of p53 is altered in HPV-associated oral carcinogenesis.…”

Section: Mdm2 and Its Association With P53mentioning

confidence: 99%

“…[51] Hu et al observed that the Pro form of the p53 gene has significantly higher levels of apoptosis compared to the Arg form in primary lymphocytes. [52] Moreover, the Arg form of the p53 gene was associated with poor apoptosis in head and neck tumours. Such tissue-specific function of this polymorphism may explain why most epidemiological studies remain inconclusive.…”

Section: Oral Lichen Planus and P53 Polymorphismmentioning

confidence: 99%

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p53 Polymorphism in Oral Lichen Planus

Vijayan

Muthukrishnan

2022

ijhs

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Oral lichen planus (OLP) is a chronic relapsing inflammatory disease. It involves T-lymphocyte aggression targeted to the basal layer of the oral mucosa.p53 plays a central role in the prevention of normal cells from the development of the malignant phenotype. Somatic alterations in p53 cause defects in normal p53 function. Wild type p53 plays an important role in repairable damage of the cell due to cellular stresses. Over-expression, as well as polymorphic variants of Mouse double minute 2(MDM2), have effects on p53 disturbances. In addition, degradation of p53 by E6 protein of high-risk human papillomavirus is also suggested as one of the mechanisms which attenuate p53 responses in oral carcinogenesis. Polymorphisms in p53 are anticipated to cause measurable disturbance in p53 function. Polymorphism of the codon 72 on exon 4 of the gene p53 is the first and most commonly known polymorphism of this gene. The presence of p53 has been demonstrated as an early marker of dysplasia in Oral Potentially Malignant Disorders. This review discusses about p53 polymorphism as an early indicator of genetic predisposition to neoplastic transformation in OLP.

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The association between high‐risk human papillomavirus and oral lichen planus

Mohammadi

Abbaszadeh

Mohtasham

et al. 2023

Clinical & Exp Dental Res

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Objectives: Oral lichen planus (OLP) is a cell-mediated inflammatory mucosal disorder and is classified as an oral potentially malignant disorder. Some research has shown that apoptosis in OLP cells is similar to a viral infection such as human papillomavirus (HPV). So, the aim of this case-control study was to investigate the association of high-risk HPV with OLP.Material and Methods: DNA was extracted from 25 formalin-fixed, paraffin-embedded (FFPE) OLP tissues and 25 FFPE normal oral tissues as case and control groups, respectively. The presence of high-risk HPV16 and HPV18 DNA was investigated by polymerase chain reaction (PCR). p-value<.05 was considered significant.Results: Twelve samples (48%) of OLPs were positive for HPV16, compared with six samples (24%) of controls; although the difference was not significant, it was borderline (p = .07). Three samples (12%) of OLPs were positive for HPV18 compared with one sample (4%) of controls; the difference was not significant (p = .3). The total frequency of both high-risk HPV were 14 samples (56%) of OLPs and 7 samples (28%) of controls; there was a significant association between the high-risk HPV and OLP (p = .04). High-risk HPVs was more prevalent in erosive-atrophic (EA) form of OLP as compared to non-EA form, although the difference was not significant (p = .13). Conclusions:The results suggest a significant association between high-risk HPVs and OLP.

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Molecular Detection of Human Papillomavirus DNA in Oral Lichen Planus Patients

Cited by 9 publications

References 0 publications

PCR-based Evaluation of Human Papillomavirus Genotypes in Oral Lichen Planus

PCR-based Evaluation of Human Papillomavirus Genotypes in Oral Lichen Planus

p53 Polymorphism in Oral Lichen Planus

The association between high‐risk human papillomavirus and oral lichen planus

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