Molecular Detection of<i>Anaplasma phagocytophilum</i>in Ixodid Ticks in Hebei Province, China

Zou, Yaxue; Jin, Hongtao; Wang, Qiuyue; Fu, Zhixin; Gao, Hongwei; Liu, Pengpeng; Liu, Quan; Chen, Lifeng

doi:10.1089/vbz.2010.0253

Cited by 18 publications

(12 citation statements)

References 19 publications

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“…Our results are consistent with other PCR studies from China [1, 6–10, 12, 13] showing that domestic ruminants from the country are infected with a range of Anaplasma and Ehrlichia spp. Differences in the prevalence of animals on the farms we studied were most likely due to differing husbandry practices and tick exposure, with dairy animals and water buffaloes raised intensively having the lowest levels of positivity.…”

Section: Discussionsupporting

confidence: 93%

“…we detected with our 16S rRNA FRET-qPCR, we could confirm the accuracy of the qPCR in detecting A. marginale and A. ovis . Anaplasma marginale was one of the most common Anaplasma species we detected with the 16S rRNA FRET-qPCR along with A. phagocytophilum which has also already been described in China where it appears to be common in ruminants [7, 11, 12, 34]. Anaplasma phagocytophilum is the agent of tick-borne fever of ruminants and is transmitted by Dermacentor silvarum , Haemaphysalis concinna, H. longicornis , and Ixodes persulcatus in China [35, 36].…”

Section: Discussionmentioning

confidence: 66%

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Molecular Detection of Anaplasma spp. and Ehrlichia spp. in Ruminants from Twelve Provinces of China

Qiu

Kelly

Zhang

et al. 2016

Canadian Journal of Infectious Diseases and Medical Microbiolog

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Anaplasma spp. and Ehrlichia spp. are tick-transmitted bacteria that are of significant economic importance as they can infect large and small ruminants and also people. There is little information on anaplasmosis and ehrlichiosis in ruminants in China. 16S rRNA FRET-qPCRs were used to screen convenience whole blood samples from 2,240 domestic ruminants in 12 provinces of China for Anaplasma spp. and Ehrlichia spp. Positive samples were further analyzed with a standard PCR for the gltA. Anaplasma spp. DNA was detected in the sheep (11.7%; 13/111), goats (81.8%; 219/270), cattle (13.2%; 241/1,830), and water buffaloes (6.9%; 2/29). Ehrlichia spp. DNA was detected in sheep (1.8%; 2/111), goats (1.1%; 3/270), and cattle (3.6%; 65/1830) but not in water buffaloes (0/29). Sequencing of gltA PCR products showed that A. marginale, A. ovis, Ehrlichia canis, and Ehrlichia sp. (JX629807) were present in ruminants from China, while the 16S rRNA FRET-qPCR sequence data indicated that there might also be A. platys, A. phagocytophilum, Anaplasma sp. BL126-13 (KJ410243), and Anaplasma sp. JC3-6 (KM227012). Our study shows that domestic ruminants from China are not uncommonly infected with a variety of Anaplasma spp. and Ehrlichia spp.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 66%

Molecular Detection of Anaplasma spp. and Ehrlichia spp. in Ruminants from Twelve Provinces of China

Qiu

Kelly

Zhang

et al. 2016

Canadian Journal of Infectious Diseases and Medical Microbiolog

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“…Although, A. phagocytophilum was detected in the genera of Ixodes , Dermacentor , and Haemaphysalis , I. persulcatus may play the most important role in the transmission of the bacterium in northeastern China, due to the high infection rate in the tick species and its high abundance. In Hebei province, A. phagocytophilum was detected in H. longicornis and D. nuttalli , where H. longicornis is the predominant tick species for the transmission of this pathogen (Yaxue et al, 2011). Phylogenetically, A. phagocytophilum detected in the study was more likely to infect ruminants (Yang et al, 2015a).…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China

et al. 2016

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Tick-borne diseases are considered as emerging infectious diseases in humans and animals in China. In this study, Ixodes persulcatus (n = 1699), Haemaphysalis concinna (n = 412), Haemaphysalis longicornis (n = 390), Dermacentor nuttalli (n = 253), and Dermacentor silvarum (n = 204) ticks were collected by flagging from northeastern China, and detected for infection with Anaplasma, Ehrlichia, Babesia, and Hepatozoon spp. by using nested polymerase chain reaction assays and sequencing analysis. Anaplasma phagocytophilum was detected in all tick species, i.e., I. persulcatus (9.4%), H. longicornis (1.9%), H. concinna (6.5%), D. nuttalli (1.7%), and D. silvarum (2.3%); Anaplasma bovis was detected in H. longicornis (0.3%) and H. concinna (0.2%); Ehrlichia muris was detected in I. persulcatus (2.5%) and H. concinna (0.2%); Candidatus Neoehrlichia mikurensis was only detected in I. persulcatus (0.4%). The Ehrlichia variant (GenBank access number KU921424), closely related to Ehrlichia ewingii, was found in H. longicornis (0.8%) and H. concinna (0.2%). I. persulcatus was infected with Babesia venatorum (1.2%), Babesia microti (0.6%), and Babesia divergens (0.6%). Additionally, four Babesia sequence variants (GenBank access numbers 862303–862306) were detected in I. persulcatus, H. longicornis, and H. concinna, which belonged to the clusters formed by the parasites of dogs, sheep, and cattle (B. gibsoni, B. motasi, and B. crassa). Two Hepatozoon spp. (GenBank access numbers KX016028 and KX016029) associated with hepatozoonosis in Japanese martens were found in the collected ticks (0.1–3.1%). These findings showed the genetic variability of Anaplasma, Ehrlichia, Babesia, and Hepatozoon spp. circulating in ticks in northeastern China, highlighting the necessity for further research of these tick-associated pathogens and their role in human and animal diseases.

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“…It is reported that this tick species transmitted a variety of pathogens [33,34], including Babesia microti [35], Ehrlichia chaffeensis [36], Anaplasma bovis [37], A. phagocytophilum [38], C. burnetii [39], Spotted fever group rickettsiae [40], and Borrelia burgdorferi [41]. With regard to the human health and economic significance, pathogens carried by H. longicornis have been well investigated in China [42-47].…”

Section: Introductionmentioning

confidence: 99%

Microbial communities and symbionts in the hard tick Haemaphysalis longicornis (Acari: Ixodidae) from north China

Liu

et al. 2013

Parasites Vectors

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BackgroundClose relationships between ticks and microbial communities are important for tick fitness and pathogen colonization and transmission. Haemaphysalis longicornis, distributed widely in China, can carry and transmit various pathogens and pose serious damages to public health and economics. However, little is known about the broader array of microbial communities and symbionts in H. longicornis under natural conditions. In the present study, we investigated the composition of bacterial communities associated with H. longicornis and evaluated the putative symbionts.MethodsThe eubacterial 16S rRNA gene clone libraries of H. longicornis were constructed and analyzed by restriction fragment length polymorphism (RFLP) and DNA sequencing. In addition, diagnostic PCR was performed to assess the prevalence, vertical transmission and infection sites of the symbionts in H. longicornis.ResultsVertically-transmitted symbionts, potential pathogens and allochthonous nonpathogenic bacteria were identified from the field-collected H. longicornis. Three types of symbionts (Coxiella-like, Arsenophonus-like and Rickettsia-like symbionts) were identified in a single host simultaneously. A series of analyses revealed the vertical transmission, prevalence, and infection sites of these symbionts. However, only Coxiella-like bacteria were transmitted stably in the laboratory-reared ticks. In addition, we identified a novel Coxiella-like agent with 95.31% sequence similarity to the taxon described previously.ConclusionsThe present study demonstrated that natural H. longicornis harboured a diverse array of microbial communities. Three types of symbionts were identified in a single host simultaneously. Moreover, high prevalence, vertical transmission and the infection sites supported an obligate symbiotic association between Coxiella symbiont and its host. The role of Coxiella symbiont in the host fitness and the interaction among microbial communities remained to be elucidated. Our investigation of microbial communities in the ticks revealed the complexity of ecological interactions between host and microbe and provided insight for the biological control of ticks.

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Molecular Detection ofAnaplasma phagocytophilumin Ixodid Ticks in Hebei Province, China

Cited by 18 publications

References 19 publications

Molecular Detection of Anaplasma spp. and Ehrlichia spp. in Ruminants from Twelve Provinces of China

Molecular Detection of Anaplasma spp. and Ehrlichia spp. in Ruminants from Twelve Provinces of China

Molecular Detection and Characterization of Zoonotic and Veterinary Pathogens in Ticks from Northeastern China

Microbial communities and symbionts in the hard tick Haemaphysalis longicornis (Acari: Ixodidae) from north China

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