Molecular detection of <i>Enterocytozoon bieneusi</i> in alpacas (<i>Vicugna pacos</i>) in Xinjiang, China

Zhang, Qiyuan; Wang, Haiyan; Zhao, Aiyun; Zhao, Wei; Wei, Zilin; Li, Zhiguo; Qi, Meng

doi:10.1051/parasite/2019031

Cited by 9 publications

(5 citation statements)

References 18 publications

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“…Such as in brown rats in different provinces in China, which was ranged from 2.9% to 14.7% [8,14,16,23,24]. This phenomenon has also been reported in other animals, for example, in alpacas (Vicugna pacos) in China (0 -42.9%) [25] and in Asiatic black bear (Ursus thibetanus) in China (0 -50%) [26]. The difference may be related to geographical environments and feeding density.…”

Section: Discussionsupporting

confidence: 55%

Host-adaptation of Rare Enterocytozoon bieneusi genotype CHN4 in Coypus (Myocastor coypus) in China

Cao

Wang³

et al. 2020

Preprint

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Background: Enterocytozoon bieneusi is a zoonotic gastrointestinal pathogen and can infect both humans and animals. Coypus (Myocastor coypus) are semi-aquatic rodents, in which few E. bieneusi infections have been reported and the distribution of genotypes and zoonotic potential remains unknown.Methods: A total of 308 fresh fecal samples were collected from seven coypu farms in China to determine the infection rate and the distribution of genotypes of E. bieneusi from coypus using nested-PCR amplification of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene.Results: E. bieneusi was detected with an infection rate of 41.2% (n = 127). Four genotypes were identified, including three known genotypes: CHN4 (n = 111), EbpC (n = 8) and EbpA (n = 7) and a novel genotype named CNCP1 (n = 1). Conclusions: The rare genotype CHN4 was the most common one in the present study, and the transmission dynamics of E. bieneusi in coypus were different from other rodents. This is the first report of E. bieneusi infections in coypus in China. Our study reveals that E. bieneusi in coypus may be potential infection source to humans.

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Section: Discussionsupporting

confidence: 55%

Host-adaptation of Rare Enterocytozoon bieneusi genotype CHN4 in Coypus (Myocastor coypus) in China

Cao

Wang³

et al. 2020

Preprint

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“…In China, genotype D has been identified in immunocompromized patients and in children with diarrhea [18,[27][28][29]32]. Genotype D has also been identified in a wide range of animal hosts in China, including non-human primates, rodents (mice, rats, squirrels, chipmunks, chinchillas, and bamboo rats), other mammals (pigs, cattle, sheep, goats, alpacas, horses, donkeys, rabbits, dogs, cats, foxes, deer, takins, minks, raccoon dogs, raccoons, lions, and hippos), and birds, as well as in water samples [6,8,9,11,17,24,26,30,31,33,34].…”

Section: Resultsmentioning

confidence: 99%

First detection and genotyping of Enterocytozoon bieneusi in pet fancy rats (Rattus norvegicus) and guinea pigs (Cavia porcellus) in China

Wang

Zhao

et al. 2020

Parasite

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Enterocytozoon bieneusi, an obligate intracellular microsporidian parasite, can infect humans and a wide variety of animals worldwide. However, information on the prevalence and molecular characterization of E. bieneusi in pet rats and guinea pigs is lacking. In this study, 325 fecal samples were collected from 152 pet fancy rats and 173 pet guinea pigs purchased from pet shops in Henan and Shandong provinces. The prevalence of E. bieneusi was 11.2% (17/152) in pet fancy rats and 20.2% (35/173) in pet guinea pigs. Genotypes D (n = 12), Peru11 (n = 3), S7 (n = 1) and SCC-2 (n = 1) were identified in pet fancy rats, and genotype S7 (n = 30) and a novel genotype PGP (n = 5) were identified in pet guinea pigs. The ITS sequence and its phylogenetic analysis showed that the novel genotype PGP was distinctly different; it exhibited less than 50% similarity to the reference sequences, and did not cluster with any of the known E. bieneusi genotype groups, forming a unique branch between groups 6 and 7. These data suggest that this is a new E. bieneusi genotype group. This is the first report of E. bieneusi infection in pet fancy rats and pet guinea pigs worldwide. The identification of zoonotic genotypes D, Peru11, and S7 suggests that pet fancy rats and guinea pigs can be potential sources of human microsporidiosis.

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“…Geographical location-based variation in the prevalence of E. bieneusi in rodents has been reported such as in brown rats in different provinces in China, which was ranged between 2.9-14.7% [8,14,16,23,24]. This phenomenon has also been reported in other animals, for example, in alpacas (Vicugna pacos) in China (0-42.9%) [25] and in Asiatic black bear (Ursus thibetanus) in China (0-50%) [26]. The difference may be related to geographical environments and feeding density.…”

Section: Discussionmentioning

confidence: 74%

Host-adaptation of the rare Enterocytozoon bieneusi genotype CHN4 in Myocastor coypus (Rodentia: Echimyidae) in China

Cao

Wang

et al. 2020

Parasites Vectors

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Background Enterocytozoon bieneusi is a zoonotic gastrointestinal pathogen and can infect both humans and animals. The coypu (Myocastor coypus) is a semi-aquatic rodent, in which few E. bieneusi infections have been reported and the distribution of genotypes and zoonotic potential remains unknown. Methods A total of 308 fresh fecal samples were collected from seven coypu farms in China to determine the infection rate and the distribution of genotypes of E. bieneusi from coypus using nested-PCR amplification of the internal transcribed spacer (ITS) region of the ribosomal RNA (rRNA) gene. Results Enterocytozoon bieneusi was detected with an infection rate of 41.2% (n = 127). Four genotypes were identified, including three known genotypes (CHN4 (n = 111), EbpC (n = 8) and EbpA (n = 7)) and a novel genotype named CNCP1 (n = 1). Conclusions The rare genotype CHN4 was the most common genotype in the present study, and the transmission dynamics of E. bieneusi in coypus were different from other rodents. To the best of our knowledge, this is the first report of E. bieneusi infections in coypus in China. Our study reveals that E. bieneusi in coypus may be a potential infection source to humans.

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Molecular detection of Enterocytozoon bieneusi in alpacas (Vicugna pacos) in Xinjiang, China

Cited by 9 publications

References 18 publications

Host-adaptation of Rare Enterocytozoon bieneusi genotype CHN4 in Coypus (Myocastor coypus) in China

Host-adaptation of Rare Enterocytozoon bieneusi genotype CHN4 in Coypus (Myocastor coypus) in China

First detection and genotyping of Enterocytozoon bieneusi in pet fancy rats (Rattus norvegicus) and guinea pigs (Cavia porcellus) in China

Host-adaptation of the rare Enterocytozoon bieneusi genotype CHN4 in Myocastor coypus (Rodentia: Echimyidae) in China

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