Molecular detection of
            <i>Salmonella</i>
            serovars Enteritidis, Heidelberg and Typhimurium directly from pre-enriched poultry samples

Souza, Margarida Neves; Lehmann, Fernanda Kieling Moreira; Carli, Sílvia De; Kipper, Diéssy; Fonseca, André Salvador Kazantzi; Ikuta, Nilo; Lunge, Vagner Ricardo

doi:10.1080/00071668.2019.1614525

Cited by 19 publications

(19 citation statements)

References 29 publications

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“…Previous studies have already demonstrated that PCR methods can be used to detect foodborne pathogens in chicken and other poultry samples (Alonso et al, 2011;Park et al, 2014). However, they have been carried out only after bacterial preenrichment and/or isolation (Borges et al, 2019;Souza et al, 2019;Borges et al, 2020). The present study demonstrated that Salmonella, Campylobacter, and C. perfringens can be detected directly from broilers' cecal samples obtained in slaughter houses.…”

Section: Discussionmentioning

confidence: 50%

“…Thus, DNA-based methods like real-time polymerase chain reaction (qPCR) and loop-mediated isothermal amplification (LAMP) have been increasingly developed and used to detect and quantify foodborne pathogens in the poultry production chain (Souza et al, 2019;Waldman et al, 2020). Other important advantage of DNA-based methods is the possibility of performing qualitative and quantitative analysis of food pathogens in biological samples simply with prior enrichment or even directly from biological samples (Albini et al, 2008;Rodgers et al, 2012;Park et al, 2014;Ricke et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

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Direct Detection and Quantification of Bacterial Pathogens from Broiler Cecal Samples in the Slaughter Line by Real-Time PCR

Souza

Wolf

Zanetti

et al. 2022

Braz. J. Poult. Sci.

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Chicken meat is an important source of foodborne pathogens, including Salmonella, Campylobacter, and Clostridium perfringens. These bacteria can occur in the intestinal microbiota of broilers and contaminate chicken carcasses in industrial meat processing. This study aimed to develop and evaluate a procedure based on real-time PCRs for the direct detection and quantification of these three bacteria in broilers' ceca collected in poultry slaughter houses and demonstrate the occurrence of these important foodborne pathogens in Brazilian poultry production flocks. Cecal contents were collected from 45 different broiler flocks in three different slaughterhouses in the state of Paraná, Brazil, totaling 45 samples (in pools of 10 different ceca/ chickens per broiler flock). Then, these samples were tested for the detection and quantification of Salmonella, Campylobacter, and Clostridium perfringens by real-time PCRs. The results demonstrated the occurrence of three (6.7%) positive pools for Salmonella, 20 (44.4%) for Campylobacter, and 32 (71.1%) for C. perfringens. Mean bacterial concentrations in the positive samples were 4.3log 10 cells/g for Salmonella, 6.4 log 10 cells/g for Campylobacter, and 5.5 log 10 cells/g for C. perfringens. In conclusion, Salmonella, Campylobacter, and C. perfringens could be detected and quantified directly from the broilers cecal contents collected in the slaughter line. This procedure will be certainly useful to more quickly detect these foodborne pathogens and prevent their occurrence in chicken meat and other poultry food products.

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Section: Discussionmentioning

confidence: 50%

Section: Introductionmentioning

confidence: 99%

Direct Detection and Quantification of Bacterial Pathogens from Broiler Cecal Samples in the Slaughter Line by Real-Time PCR

Souza

Wolf

Zanetti

et al. 2022

Braz. J. Poult. Sci.

View full text Add to dashboard Cite

show abstract

“…DNA‐based detection methods such as PCR can quickly obtain test results and greatly shorten clinical outbreak time. In recent years, different PCR based detection methods for some food‐borne pathogens have been developed (Alarcon, Garcia‐Canas, Cifuentes, Gonzalez, & Aznar, ; Pierce et al., ; Souza et al., ; Zhou et al., ). Traditional simplex PCR methods cannot detect multiple pathogens at the same time.…”

Section: Resultsmentioning

confidence: 99%

A multiplex PCR assay with a common primer for the detection of eleven foodborne pathogens

Tao

Liu

Ding

et al. 2020

Journal of Food Science

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Salmonella enterica, Listeria monocytogenes, Shigella flexneri, Escherichia coli O157:H7, Vibrio parahaemolyticus, Staphylococcus aureus, Vibrio cholerae, Clostridium botulinum type A, Bacillus cereus, Clostridium perfringens Alpha toxin, and Yersinia enterocolitica are 11 common foodborne pathogens. Traditional bacterial culture methods for detecting pathogens are time‐consuming and labor‐intensive. Multiplex PCR technology, which can detect multiple targets in a single tube, has been increasingly applied to microbial detection due to its high specificity, sensitivity, and fast response. This paper is to establish a multiplex PCR technology mediated by a common primer for the detection of these 11 common foodborne pathogens in order to achieve the goal of nondirectional screening for these 11 common foodborne pathogens. The specificity of the established CP‐MPCR detection system was first verified by 100 clinical isolates. The sensitivity of the CP‐MPCR detection system was then detected by using cultured bacteria preparations and has been confirmed with a high sensitivity of 103 to 104 CFU/mL, among them, the sensitivity of the CP‐MPCR for Vibrio cholerae and S. flexneri can even achieve 102 CFU/mL. Sixty anal swab samples collected from Suzhou CDC and 16 enrichment cultured solutions of food samples collected from the Suzhou Food Inspection and Testing Center were tested using the CP‐MPCR system. A total of 32 positive results were detected. Practical Application Food poisoning incidents occur frequently around the world, mainly because of the contamination of food by pathogenic bacteria and serious harm to human health. The method provided in this study can detect 11 foodborne pathogens in food, which can effectively prevent the spread of pathogenic microorganisms. At the same time, for the food poisoning incident that has already occurred, this method can be used for diagnosis to find out the cause.

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“…[7][8][9][10] Consequently, various molecular techniques such as ELISA and PCR procedures were introduced to provide higher sensitivity and selectivity in comparison with conventional microbiological isolation methods. 11 However, those techniques are still expensive and time consuming methodologies with a reported limit of detection for Salmonella of 10 4 to 10 5 CFU ml À1 and 10 4 CFU ml À1 for ELISA, 12 and PCR, 13 respectively.…”

Section: Introductionmentioning

confidence: 99%

Voltammetric determination of Salmonella typhimurium in minced beef meat using a chip-based imprinted sensor

et al. 2022

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show abstract

Molecular detection of Salmonella serovars Enteritidis, Heidelberg and Typhimurium directly from pre-enriched poultry samples

Cited by 19 publications

References 29 publications

Direct Detection and Quantification of Bacterial Pathogens from Broiler Cecal Samples in the Slaughter Line by Real-Time PCR

Direct Detection and Quantification of Bacterial Pathogens from Broiler Cecal Samples in the Slaughter Line by Real-Time PCR

A multiplex PCR assay with a common primer for the detection of eleven foodborne pathogens

Voltammetric determination of Salmonella typhimurium in minced beef meat using a chip-based imprinted sensor

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