Molecular detection of Salmonella species in bovine fecal samples

Bordonaro, Rebecca; McDonough, Patrick L.; Chang, Yung-Fu; Mohammed, Hussni O.

doi:10.1177/1040638713508123

Cited by 5 publications

(5 citation statements)

References 8 publications

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“…Another consideration for the low prevalence that we found would be the method in which the samples were tested. Some might argue that the BAX Automated System was originally designed to test for bacterial pathogens in food products; however it has shown great potential in evaluating the prevalence of bacteria in a population by testing fecal samples [11]. Fecal samples tend to be less clean than food products, and therefore it is more difficult to grow and detect Salmonella in these samples because of other species of competitive bacteria and other organisms.…”

Section: Discussionmentioning

confidence: 99%

“…Polymerase chain reaction (PCR) detection of Salmonella in these samples was performed using the BAX Automated System (DuPont Qualicon, DuPont Nutrition & Health, Molecular Diagnostics, Wilmington, DE), which has been validated for use in bovine fecal samples [11]. The protocol used for amplification prior to detection of Salmonella in these samples was a modified version of a protocol developed by DuPont Qualicon for use with the BAX system.…”

Section: Salmonella Detectionmentioning

confidence: 99%

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The Potential Risk Associated with Foodborne Pathogens in Watersheds: Salmonellap spp. in Dairy Cattle

Bordonaro¹,

McDonough²,

Chang³

et al. 2015

JWARP

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Salmonella spp. is one of the most important food-and waterborne pathogens implicated in human and animal disease. A repeat cross-sectional study was performed to assess the occurrence of Salmonella in dairy herds in a watershed and determine the factors that play a role in the likelihood of perpetuation of this organism among animals on these farms. A convenience sample of herds in the Delaware County watershed was selected based on farmers' willingness to participate. Fecal samples were collected per rectum from a representative sample of cattle in these herds and tested for the presence of Salmonella using a combination of culturing and molecular detection methods. The significance of association between the putative risk factors and the likelihood of Salmonella spp. was evaluated using the logistic regression analysis. A total of 34 dairy farms were enrolled in the study and 1987 samples were collected coinciding with seasonal variation. Salmonella spp. was detected in 44% (15/34) of the herds in the study population (i.e., from at least one animal in these herds). However, only a small fraction of the entire sampling population (1.7%) was found to be shedding Salmonella. Risk factors that were found to be associated with the presence of the pathogen on the farm in the final multivariable model included concrete flooring for calves, prevention of calf access to the dam, and the lack of a designated calving pen. Two serovars, Salmonella enterica serovar Infantis and Salmonella enterica serovar Oranienburg, were identified among the isolates recovered in this study. Our study demonstrated that there was a potential risk of watershed degradation from Salmonella associated with cattle.

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Section: Discussionmentioning

confidence: 99%

Section: Salmonella Detectionmentioning

confidence: 99%

The Potential Risk Associated with Foodborne Pathogens in Watersheds: Salmonellap spp. in Dairy Cattle

Bordonaro¹,

McDonough²,

Chang³

et al. 2015

JWARP

Self Cite

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“…After cooling, 30 μL aliquots of lysate were transferred to BAX System PCR tubes and allowed to sit for 10–30 min in a cooling block to allow full hydration of the PCR pellets. After hydration, all tubes were placed in the BAX Q7 Instrument and the full process for BAX System Real-Time PCR Assay for Salmonella was run [ 35 ]. Salmonella in positive samples was not enumerated.…”

Section: Methodsmentioning

confidence: 99%

Reduction of Pathogens in Feces and Lymph Nodes Collected from Beef Cattle Fed Lactobacillus salivarius (L28), Lactobacillus acidophilus (NP51) and Propionibacterium freudenreichii (NP28), Commercially Available Direct-Fed Microbials

Flach

Dogan

Kreikemeier

et al. 2022

Foods

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The purpose of the study was to evaluate the prevalence and concentration of foodborne pathogens in the feces and peripheral lymph nodes (PLNs) of beef cattle when supplemented with direct-fed microbials (DFMs) in feedlots. Fecal samples were collected from the pen floors over a 5-month period at three different feedlots in a similar geographical location in Nebraska, where each feed yard represented a treatment group: (i.) control: no supplement, (ii.) Bovamine Defend: supplemented with NP51 and NP24 at a target dose of 9 log10CFU/g/head/day, and (iii.) Probicon: supplemented with L28 at a target dose of 6 log10CFU/g/head/day. Each fecal sample was tested for the prevalence of E. coli O157:H7 and Salmonella, and concentration of E. coli O157:H7, Enterobacteriaceae and Clostridium perfringens. Cattle were harvested and PLNs were collected on the harvest floor. Real-time Salmonella PCR assays were performed for each PLN sample to determine Salmonella presence. The cattle supplemented with both DFMs had reduced foodborne pathogens in fecal samples, but feces collected from the pens housing the cattle supplemented with Probicon consistently had significantly less E. coli O157:H7 and Salmonella prevalence as well as a lower C. perfringens concentration. While DFMs do not eliminate foodborne pathogens in fecal shedding and PLNs, the use of DFMs as a pre-harvest intervention allows for an effective way to target multiple pathogens reducing the public health risks and environmental dissemination from cattle.

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“…The detection of E. coli O157:H7 in these samples was performed using a combination bacteriological enrichment and automated polymerase chain reaction (PCR) system detection (Bax PCR Real-Time Assay, System, Hygiena, https://www.hygiena.com/food-safety-solutions/pathogen-detection/bax-system-pcr-assays-for-e-coli-o157h7/) which has been validated for use in bovine fecal samples (Bordonaro, et al, 2013(Bordonaro, et al, , 2015Johnson et al, 1998). The detection method consisted of bacteriological enrichment and molecular detection.…”

Section: Escherichia Coli O157:h7 Detectionmentioning

confidence: 99%

The potential risk associated with foodborne pathogens in a watershed: Escherichia coli O157:H7 in dairy cattle

2023

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We carried out an investigation to determine the occurrence of Escherichia coli O157:H7 in dairy herds in the Delaware County watershed and to identify the factors that play a role in the likelihood of presence of this organism among animals on these farms. The pathogen poses risk of environmental degradation and health to the inhabitants. A total of 2162 fecal samples were collected per rectum from a representative sample of cattle on 27 dairy farms. The samples were investigated for the presence of E. coli O157:H by initially enriching using a bacteriological media and detection of the pathogen using real‐time polymerase chain reaction technique. Escherichia coli O157:H7 was detected in 74% of the herds in the target population and in 3.7% of samples collected. An additional 54 animals were identified that were infected with O157 non‐H7 strains of E. coli among 15 farms. Several putative risk factors were associated with the detection of the pathogen on the enrolled farms included age, housing calves indoors, group housing for calves, housing calves in the calf barn, presence of dogs on the farm, and housing post‐weaned calves in a cow barn or heifer barn versus a greenhouse. In conclusion, E. coli O157:H7 was present on the dairy farms of Delaware County and may pose a threat to the people that live and work there. The risk associated with the detection of this pathogen could be reduced by adjusting management factors that were identified in this study.

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Molecular detection of Salmonella species in bovine fecal samples

Cited by 5 publications

References 8 publications

The Potential Risk Associated with Foodborne Pathogens in Watersheds: <i>Salmonellap</i> spp. in Dairy Cattle

The Potential Risk Associated with Foodborne Pathogens in Watersheds: <i>Salmonellap</i> spp. in Dairy Cattle

Reduction of Pathogens in Feces and Lymph Nodes Collected from Beef Cattle Fed Lactobacillus salivarius (L28), Lactobacillus acidophilus (NP51) and Propionibacterium freudenreichii (NP28), Commercially Available Direct-Fed Microbials

The potential risk associated with foodborne pathogens in a watershed: Escherichia coli O157:H7 in dairy cattle

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Molecular detection of Salmonella species in bovine fecal samples

Cited by 5 publications

References 8 publications

The Potential Risk Associated with Foodborne Pathogens in Watersheds: &lt;i&gt;Salmonellap&lt;/i&gt; spp. in Dairy Cattle

The Potential Risk Associated with Foodborne Pathogens in Watersheds: &lt;i&gt;Salmonellap&lt;/i&gt; spp. in Dairy Cattle

Reduction of Pathogens in Feces and Lymph Nodes Collected from Beef Cattle Fed Lactobacillus salivarius (L28), Lactobacillus acidophilus (NP51) and Propionibacterium freudenreichii (NP28), Commercially Available Direct-Fed Microbials

The potential risk associated with foodborne pathogens in a watershed: Escherichia coli O157:H7 in dairy cattle

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The Potential Risk Associated with Foodborne Pathogens in Watersheds: <i>Salmonellap</i> spp. in Dairy Cattle

The Potential Risk Associated with Foodborne Pathogens in Watersheds: <i>Salmonellap</i> spp. in Dairy Cattle