Molecular detection of isoniazid monoresistance improves tuberculosis treatment: A retrospective cohort in France

Bachir, Marwa; Guglielmetti, Lorenzo; Tunesi, Simone; Billard-Pomarès, Typhaine; Chiesi, Sheila; Jaffré, Jérémy; Langris, Hugo; Pourcher, Valérie; Schramm, Frédéric; Lemaître, Nadine; Robert, Jérôme

doi:10.1016/j.jinf.2022.05.017

Cited by 8 publications

(8 citation statements)

References 21 publications

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“…Among the 23 patients with HR-TB, 10 (43.5%) had smear positive samples, and the median [IQR] time to positivity of the culture was 11 days [6][7][8][9][10][11][12][13][14][15][16]. According to p-DST, 8 (34.7 %) patients had low-level H resistance and 15 (65.3 %) had high-level H resistance.…”

Section: Epidemiological and Clinical Characteristics Of Hr-tb Patientsmentioning

confidence: 99%

“…According to p-DST, 8 (34.7 %) patients had low-level H resistance and 15 (65.3 %) had high-level H resistance. The median [IQR] delay between sampling and g-DST performed on cultured Mtb was 15.5 days [10][11][12][13][14][15][16][17][18][19][20] and was significantly lower than the median [IQR] delay between sampling and p-DST of 36 days [30-50] (p<0.0001).…”

Section: Epidemiological and Clinical Characteristics Of Hr-tb Patientsmentioning

confidence: 99%

“…The median (min-max) duration of these simplified regimens (from treatment modification to the end of anti-TB therapy) was 6 months (2-20). Overall, the median [IQR] treatment duration was 9 [6][7][8][9][10][11][12] months including 6 (26.1%) patients treated for 6 months, 8 (34.7%) patients treated for 9 months, and 7 (30.4%) treated > 9 months (range10 to 22). In 8 patients, a longer therapy was explained by the extrapulmonary localisation and/or by risk factors of treatment failure (extensive cavitary disease and/or slow conversion of smear/culture).…”

Section: Treatment and Outcome Of Hr-tb Patientsmentioning

confidence: 99%

“…France is a high-income country with low prevalence of TB cases (less than 10/100,000 inhabitants); in a previous retrospective study from 2016 and 2017, only 26% of patients diagnosed with TB had rapid molecular detection of H resistance, which correlated with early adaptation of HR-TB treatment 11 . Thus, the first objective of the present study was to explore the impact of rapid resistance detection tests, including NGS-based approach on the management of the patients and on the TB disease outcome, with a focus on HR-TB cases.…”

Section: Introduction (628 Words)mentioning

confidence: 99%

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Rapid resistance detection is reliable for prompt adaptation of isoniazid resistant tuberculosis management

Bahuaud,

Genestet,

Hodille

et al. 2023

Preprint

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ObjectivesAppropriate tuberculosis (TB) management requires anti-TB drugs resistance detection. We assessed the performance of rapid resistance detection assays and their impact on treatment adaptation, focusing on isoniazid resistant (HR) TB.MethodsFrom 2016 to 2022, all TB cases enrolled in 3 hospitals were reviewed for phenotypic drug susceptibility testing (p-DST) and genotypic DST (g-DST) performed by rapid molecular testing, and next generation sequencing (NGS). Clinical characteristics, treatment and outcome were collected for HR-TB patients. The concordance between g-DST and p-DST results, and delay between treatment initiation and results of g-DST and p-DST were respectively recorded to assess the contribution of DST results on HR-TB management.ResultsAmong 654 TB cases enrolled, 29 were HR-TB. Concordance between g-DST by rapid molecular methods and p-DST was 76.9%, whilst concordance between NGS-based g-DST and p-DST was 98.7%. Rapid resistance detection significantly fastened HR-TB treatment adaptation (median delay between g-DST results and treatment modification was 6 days). It consisted in fluoroquinolone implementation for 17/23 patients; outcome was favourable except for 2 patients who died before DST reporting.ConclusionRapid resistance detection fastened treatment adaptation. Also, NGS-based g-DST showed almost perfect concordance with p-DST, thus providing rapid and safe culture-free DST alternative.

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Section: Epidemiological and Clinical Characteristics Of Hr-tb Patientsmentioning

confidence: 99%

Section: Epidemiological and Clinical Characteristics Of Hr-tb Patientsmentioning

confidence: 99%

Section: Treatment and Outcome Of Hr-tb Patientsmentioning

confidence: 99%

Section: Introduction (628 Words)mentioning

confidence: 99%

See 2 more Smart Citations

Rapid resistance detection is reliable for prompt adaptation of isoniazid resistant tuberculosis management

Bahuaud,

Genestet,

Hodille

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

“…France is a high-income country with low prevalence of TB cases (less than 10/100,000 inhabitants); in a previous retrospective study from 2016 to 2017, only 26 % of patients diagnosed with TB had rapid molecular detection of H resistance, which correlated with early adaptation of Hr-TB treatment [ 11 ]. Thus, the first objective of the present study was to explore the impact of rapid resistance detection tests, including NGS-based approach on the management of the patients and on the TB disease outcome, with a focus on Hr-TB cases.…”

Section: Introductionmentioning

confidence: 99%

Rapid resistance detection is reliable for prompt adaptation of isoniazid resistant tuberculosis management

Bahuaud,

Genestet,

Hodille

et al. 2024

Heliyon

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Rational tuning of binding properties of pillar [5] arene-based sensing material by synergistic effect and its application for fluorescent turn-on detection of isoniazid and controlled reversible morphology

Hu,

Zhao,

Lin

et al. 2024

Analytica Chimica Acta

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Molecular detection of isoniazid monoresistance improves tuberculosis treatment: A retrospective cohort in France

Cited by 8 publications

References 21 publications

Rapid resistance detection is reliable for prompt adaptation of isoniazid resistant tuberculosis management

Rapid resistance detection is reliable for prompt adaptation of isoniazid resistant tuberculosis management

Rapid resistance detection is reliable for prompt adaptation of isoniazid resistant tuberculosis management

Rational tuning of binding properties of pillar [5] arene-based sensing material by synergistic effect and its application for fluorescent turn-on detection of isoniazid and controlled reversible morphology

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