Molecular Detection of Jujube Witches' Broom Phytoplasmas in Micropropagated Jujube Shoots

Tian, Janbao; Guo, H.P.; Bertaccini, Assunta; Martini, Marta; Paltrinieri, Samanta; Pastore, M.

doi:10.21273/hortsci.35.7.1274

Cited by 13 publications

(10 citation statements)

References 8 publications

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“…Phytoplasma reference strains maintained in periwinkle (Bertaccini, 2003) were tomato big bud from Australia (TBB; 16SrII‐B), alder yellows (ALY; 16SrV‐C), elm yellows (EY1; 16SrV‐A), elm witches’ broom (ULW; 16SrV‐A), rubus stunt (RuS; 16SrV‐C), German stone fruit yellows (GSFY/2; 16SrX‐B), pear decline (PD; 16SrX‐C), apple proliferation (AP15; 16SrX‐A) and stolbur (STOL; 16SrXII‐A). Jujube witches’ broom (JWB; 16SrV‐B) (Tian et al., 2000) in micropropagated jujube was also used.…”

Section: Reference Phytoplasma Strainsmentioning

confidence: 99%

Identification of Phytoplasmas Associated with Grapevine Yellows in Serbia

Duduk¹,

Botti²,

Ivanović³

et al. 2004

Journal of Phytopathology

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The molecular identification and characterization of phytoplasmas from infected grapevines in four locations in Serbia are reported. Phytoplasmas were detected and identified by restriction fragment length polymorphism (RFLP) analysis of polymerase chain reaction (PCR) amplified 16S rDNA. Grapevine yellows were associated with three molecularly distinguishable phytoplasmas: Flavescence dore´e phytoplasmas (elm yellows group: 16SrV-C subgroup) were present only in the Ž upa Aleksandrovac region; Bois noir phytoplasmas (stolbur group: 16SrXII-A subgroup) were detected in the other surveyed regions; a mixed infection of European stone fruit yellows (apple proliferation group: 16SrX-B subgroup) and Bois noir phytoplasmas was identified in one sample. A finer molecular characterization by RFLP analysis of rpS3 and SecY genes of Flavescence dore´e phytoplasmas from Ž upa Aleksandrovac confirmed that the Serbian genotype is indistinguishable from a strain from the Veneto region, Italy. Characterization of the tuf gene of Bois noir phytoplasmas showed lack of amplification of samples from Erdevik. HpaII profiles of tuf gene PCR products of samples from Pali and Radmilovac were identical, and were indistinguishable from one of the two profiles produced by samples from Italian grapevines used as reference strains.

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Section: Reference Phytoplasma Strainsmentioning

confidence: 99%

Identification of Phytoplasmas Associated with Grapevine Yellows in Serbia

Duduk¹,

Botti²,

Ivanović³

et al. 2004

Journal of Phytopathology

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“…In total, 31 jujube samples were tested and on 11 of them non-ribosomal gene characterization was also carried out, samples were from original field collected and micro-propagated materials (Table 1). Control strains used were jujube witches' broom strain ZF23 in jujube (16SrV-B; Tian et al, 2000), and in periwinkle: elm yellows (EY) and elm witches' broom (ELMWB) (16SrV-A), carrot yellows (CA) (16SrI-C); aster yellows (AY-27) (16SrI-B), and primula virescence (PRIVA) (16SrI-B).…”

Section: Methodsmentioning

confidence: 99%

Multigene characterization of phytoplasmas infecting jujube and paulownia in China

Zhao¹,

Contaldo²,

Paltrinieri³

et al. 2016

Phyt. Moll.

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Molecular characterization of phytoplasmas in original and micropropagated jujube and paulownia samples collected from plants showing witches' broom symptoms was carried out. Ribosomal (16S rRNA) and non-ribosomal genes of the phytoplasma associated with the two diseases were studied after amplification by nested-PCR, using restriction fragment length polymorphism analyses with informative endonucleases. The phytoplasmas infecting paulownia were identified as belonging to ribosomal subgroup 16SrI-D, while those infecting jujube belonged to 16SrV-B. Further molecular characterization show little variability in the other studied genes of these phytoplasmas between original and micropropagated plant material, indicating stability of the population of pathogenic strains of these two phytoplasma when derived from the same original plant.

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“…As reference phytoplasmas strains representing all described 16SrV ribosomal RNA subgroups, multiplied in periwinkle were employed: alder yellows, ALY; elm yellows, EY1; elm witches’‐broom, ULW; rubus stunt, RuS. As additional control strain, the Jujube Witches’‐Broom (JWB) phytoplasma was grown in vitro on jujube shoots (Tian et al. 2000).…”

Section: Methodsmentioning

confidence: 99%

Grapevine yellows in Northern Italy: molecular identification of Flavescence dorée phytoplasma strains and of Bois Noir phytoplasmas

Botti

Bertaccini

2007

Journal of Applied Microbiology

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Aims: Verify the presence and the molecular identity of phytoplasmas in Northern and Central Italy vineyards where yellows diseases are widespread. Methods and Results: Phytoplasma presence and identity were determined by PCR/RFLP analyses on 16S ribosomal gene testing 1424 symptomatic samples. The 65% of samples resulted phytoplasma infected; in particular 256 samples were found positive to phytoplasmas belonging to group 16SrV (mainly Flavescence dorée associated), and the remaining 37% was infected by phytoplasmas belonging to ribosomal subgroup 16SrXII‐A (Stolbur or Bois Noir associated). 16SrV ribosomal group representative strains were further typed for variability in SecY and rpS3 genes. The results showed the presence of phytoplasmas belonging to 16SrV‐C, 16SrV‐D and to a lesser extent, 16SrV‐A subgroup. Conclusions: Possible relationships between genetic polymorphisms of phytoplasma strains belonging to subgroup 16SrV‐C and their geographic distribution and/or epidemic situations were detected. Significance and Impact of the Study: Bois Noir and Flavescence dorée phytoplasmas are present in significant percentages in the areas under investigation. Molecular tools allowed to identify phytoplasma‐infected plants and the genes employed as polymorphism markers resulted useful in distinguishing and monitoring the spreading of the diseases associated with diverse phytoplasmas belonging to 16SrV subgroup in vineyards.

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Molecular Detection of Jujube Witches' Broom Phytoplasmas in Micropropagated Jujube Shoots

Cited by 13 publications

References 8 publications

Identification of Phytoplasmas Associated with Grapevine Yellows in Serbia

Identification of Phytoplasmas Associated with Grapevine Yellows in Serbia

Multigene characterization of phytoplasmas infecting jujube and paulownia in China

Grapevine yellows in Northern Italy: molecular identification of Flavescence dorée phytoplasma strains and of Bois Noir phytoplasmas

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