Molecular Detection of Klebseilla pneumoniae Isolated from Respiratory Infected Sheep and Histopathological Study in Rabbits

Abstract

ESPIRATORY disease is common in rabbits, which is frequently difficult to detectable in live animals until it becomes serious and complicated. This study was aimed to describe the histopathological changes of the lung, liver and kidney of rabbits after induction of infection by Klebseilla pneumoniae which is isolated from respiratory system infected sheep that detection by using routine diagnostic methods followed identification by using the Vitek2 system and by molecular detection of K. pneumonaie isolates with PCR using (16S rRNA gene). This experimental study has six rabbits; two animals for control and four animals for infection. After induction of infection had used of euthanasia to six rabbits and opened the thoracic cavity in laboratory. The organs were filmed. The maine pathological lesions had seen in lung, liver and kidney of rabbits which infected with Klebsiella pneumoniae. Histological and morphological description of respiratory tract of rabbits were achieved, The trachea supported by double cartilage. The rabbits are valuable models in respiratory research due to their respiratory hyper responsiveness to asthma resembles in humans. The histopathological changes of lung rabbit infected with K. pneumoniae showing mild thickness of alveoli emphysema (atelectasis). The histopathological changes of liver showed hydropic degeneration in the cytoplasm of hepatocytes with infiltration of inflammatory cell in the parenchyma of liver and congestion of blood vessels. The Histopathological changes of kidney reveals infiltration of inflammatory cell congestion and stenosis of renal lead to atrophy of renal tubules. The results of antimicrobial susceptibility showed that all 7 isolates (100%) of K. pneumonaie were susceptible to both imipenem and meropenem.