Molecular detection of Leishmania infantum DNA according to clinical stages of leishmaniasis in dog

Abstract

The aim of this study was to compare molecular tests used to diagnose Leishmania spp. in dogs with different stages of infection. Blood and conjunctival swab (CS) samples from dogs classified in four clinical stages were subjected to different PCR protocols (13A/13B, MC1/MC2, LITSR/L5.8S and LEISH-1/LEISH-2 primers). To the study, 22.3% (48/215) of dogs were classified as without clinical signs, 67.5% (145/215) stage I (mild disease), 7.0% (15/215) stage II (moderate disease) and 3.2% (7/215) stage III (severe disease). The results showed that in blood samples, 13A/13B detected a significant higher number of positive dogs in stage I (25/145) and in total (42/215) (p≤0.05). However, when CS samples were tested, no difference was observed (p>0.05). On the other hand, in blood samples, MC1/MC2 detected significantly fewer positive dogs classified as without clinical signs (0/48), in stage I (0/145) and in total (1/215) (p≤0.05). Likewise, in CS samples, this primers showed also lower detection (1/215) (p≤0.05). So than, we can conclude that PCR on blood samples with 13A/13B primers has greater capacity to detect positive dogs, mainly at the initial of clinical disease than do other primers and MC1/MC2 are not a good choice to detect Leishmania infantum infection in dogs.

Is the Prevalence of Leishmania infantum Linked to Breeds in Dogs? Characterization of Seropositive Dogs in Ibiza

“…However, our results show no statistical difference between ages. According to studies performed in Brazil and Tunisia, the highest prevalence was found in dogs with clinical stage II, i.e., moderate disease [ 59 , 60 ].…”

Leishmania infantum in the reproductive organs of dogs

“…Sterilized ultrapure water was the negative control. We performed PCR gel electrophoresis on 1.5% agarose gels and subjected PCR products to DNA sequencing as described by FERNANDES et al (2019).…”

“…Many infected dogs remain without clinical signs; however, canine leishmaniasis (CanL) is a systemic disease characterized by nonspecific clinical signs (SOLANO-GALLEGO et al, 2011). L. infantum infection or DNA has been reported in several canine tissues, organs, and body fluids (TAFURI et al, 2004;SOLANO-GALLEGO et al, 2011;SILVA et al, 2016;FERNANDES et al, 2019), including those Silva et al present in the genital system (DINIZ et al, 2005;SILVA et al, 2008;AMARA et al, 2009;MANNA et al, 2012;BOECHAT et al, 2016;OLIVEIRA et al, 2016aOLIVEIRA et al, , 2016bMAGRO et al, 2017). In particular, infection of the canine reproductive tract has received substantial attention since venereal and vertical transmission were reported (PANGRAZIO et al, 2009;SILVA et al, 2009).…”

>b<Identificação e caracterização de tripanossomatídeos que infectam gatos domésticos (>i<Felis catus>/i<) de área endêmica para leishmaniose visceral>/b<

“…utilizando o kDNA de Leishmania spp. (KOLTAS et al, 2016;FERNANDES et al, 2019), sendo sensível o suficiente para detectar kDNA de um único organismo (RODGERS et al, 1990). Já em cães, a PCR ITS-1 de suabe se mostrou uma técnica muito eficiente na detecção de Leishmania (STRAUSS- AYALI, et al, 2004).…”

“…É importante destacar que os gatos aqui estudados pertencem a abrigos de animais, onde a ocorrência de Lu. longipalpis, LVC e LF, já foi relatada em estudos anteriores (SPADA et al, 2014;ALVES-MARTIN et al, 2017;FERNANDES et al, 2019;LEONEL et al, 2020).…”