Molecular Detection of Multidrug Resistant Staphylococcus aureus Isolated from Bovine Mastitis Milk in Bangladesh

Abstract

The current study was conducted to isolate and identify multidrug-resistant Staphylococcus aureus (MDR-SA) from mastitis milk samples and to determine their antimicrobial susceptibility pattern. A total of 48 bovine mastitis (BM) milk samples were collected from different parts of the Rangpur division, Bangladesh. After the collection of milk samples, mastitis was confirmed using the California mastitis test. Isolation and identification of Staphylococcus aureus were performed using conventional cultural and biochemical tests as well as using molecular methods of PCR. Nucleotide sequence analysis of the 23S rRNA gene of Staphylococcus aureus was determined. The antibiogram of the isolated bacteria was conducted using the disc diffusion method. Phylogenetic analysis of 23S rRNA was done using MEGA 7, ClustalW multiple sequence alignment, and NCBI-BLAST tools, where the sequence of the isolate showed 98% to 99% identity. Antibiogram test using 15 antimicrobial agents showed that all of the Staphylococcus aureus isolates were classified as multidrug-resistant (MDR). It was found that the isolates were resistant to tetracycline, novobiocin, methicillin, vancomycin, and cephradine, and the isolates were sensitive to ciprofloxacin, azithromycin, norfloxacin, levofloxacin, gentamicin, and amoxicillin. The detection of MDR-SA in mastitis milk is alarming and represents a great public health concern. The findings of the present study help identify Staphylococcus aureus at the molecular level using 23S rRNA gene sequencing and will help select the appropriate and effective antimicrobial agent to control BM in the northern part of Bangladesh.

Occurrence and Antimicrobial Resistance Pattern of E. coli O157:H7 Isolated from Foods of Bovine Origin in Dessie and Kombolcha Towns, Ethiopia

“…However, in comparison to the present study, higher prevalence rates of E. coli were reported by Salauddin et al [60] (100.0%), Baz et al [61] (96.0%), Arjyal et al [62] (92.0%), Balcha et al [63] (62.5%), Gundogan and Avci [64] (74.0%), Lingathurai and Vellathurai [65] (70.0%), Altalhi and Hassan [66] (66.0%), Chyea et al [67] (64.5%), and Ali and Abdelgadir [68] (63.0%) in Rangpur Division (Bangladesh), Kars city (Turkey), Kathmandu Valley (Nepal), Mekelle, Turkey, Madurai (South India), Taif region (Western Saudi Arabia), Malaysia, and Khartoum state, respectively.…”

Identification and antimicrobial susceptibility of methicillin-resistant Staphylococcus aureus-associated subclinical mastitis isolated from dairy cows in Bogor, Indonesia

“…A majority of MRSA strains were more resistant to multiple antibiotics among S. aureus isolates recovered from bovine mastitis cases in Shanghai and Zhejiang areas of China [29]. In Bangladesh, multidrug-resistant S. aureus isolates from bovine mastitis milk samples were tetracycline, novobiocin, methicillin, vancomycin, and cephradine resistant [30]. Here, we found all MRSA strains with the mecA gene confirmed by the PCR method to be non-susceptible to oxacillin and cefoxitin.…”

In vitro Antibacterial Evaluation of Four Selected Medicinal Plants against Staphylococcus aureus Isolated from Bovine Mastitis in Mieso District West Hararghe Zone, Oromia Regional State, Ethiopia

“…Isolation of S. aureus was undertaken following the method described by Salauddin et al [29]. A loop full of milk portion from each sample was aseptically inoculated onto a sterilized blood agar plate (BAP) endowed with 5% heparinized sheep blood, then the plates were incubated aerobically at 37 °C for 24hrs.…”