Molecular detection of Mycobacterium bovis in cattle herds of the state of Pernambuco, Brazil

Cezar, Renata Duarte da Silva; Lucena‐Silva, Norma; Filho, Antônio Fernando Barbosa Batista; Borges, Jonas de Melo; Oliveira, Pollyane Raysa Fernandes de; Lúcio, Érica Chaves; Arruda-Lima, Maíra; Santana, Vania Lucia de Assis; Pinheiro, José Wilton

doi:10.1186/s12917-016-0656-1

Cited by 19 publications

(11 citation statements)

References 27 publications

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“…32 A variable incidence of bTB may effect through several modes like the geographical position features; Agroecological system; public health condition for humans and animals; herd size, farm management and grazing practice; age, breed, gender and body condition score of animals; compelled organizing schemes of the veterinary departments; concurrent diseases, host genetic variation, immune suppression, cattle behavior, physiological status, cows scheme form, feeding system, treatments with control program, environments or weather, pathological variations. [33][34][35] Whilst the respiratory disorders, emaciation, rough hair coat, decrease in milk production and presence of reproductive failures shows the most prevalent signs of positive cows in blood and milk groups; the enlargement of lymph nodes, loss of appetites and feces abnormalities manifested the lowers, with presence of some differences between both serum groups. These resultants were approval to studies revealed by various researchers.…”

Section: Discussionmentioning

confidence: 99%

The clinical and serological diagnosis of Mycobacterium bovis in blood and milk serums of lactating cows by IDEXX ELISA test in Wasit and Dhi-Qar provinces/Iraq

Al-Fattli

2016

J. Contemp. Med. Sci.

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Objectives This study was conducted to disclose the specific antibodies against M. bovis of bovine tuberculosis (bTB) in blood and milk serum samples, with detection of the most prevalent clinical signs in positive cows. Methods In some rural areas of two Iraqi provinces (Wasit and Dhi-Qar), 119 lactating cows were submitted to the clinical examination with obtaining of blood and milk to tested by using the IDEXX ELISA test. Results The overall seroprevalence in blood and milk was (20.16%) and (15.12%), respectively. In Wasit, the prevalence was (22.85%) and (15.71%), while in Dhi-Qar, the prevalence was (16.32%) and (14.28%) in blood and milk, respectively. As well as, marked significant differences in seroprevalence were observed between and within the two study's provinces and samples. According to clinical examination, a significant rising (P > 0.05) was revealed in respiratory disorders, decreasing in milk production, emaciation, rough hair coat and repetitive reproductive problems, whilst a significant decreasing (P < 0.05) in persistent feces abnormalities, mastitis, lymph nodes enlargement and loss of appetite. Conclusion The study demonstrated, for the first time in Iraq, the efficient of IDEXX ELISA, as a screening test in the detection of bTB in lactating cows by using blood and milk serum samples, and the competence of milk, as sample, in exhibition of infection. Also, the study exposed the high infection rate of bTB in cows of rural areas of Wasit and Dhi-Qar provinces.

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Section: Discussionmentioning

confidence: 99%

The clinical and serological diagnosis of Mycobacterium bovis in blood and milk serums of lactating cows by IDEXX ELISA test in Wasit and Dhi-Qar provinces/Iraq

Al-Fattli

2016

J. Contemp. Med. Sci.

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“…Mendes et al (2011) examined dairy herds of 15 municipalities and observed that 14% (86/612) of the evaluated animals were positive for the tuberculin test. Milk and blood samples were collected from 401 dairy cattle in 20 properties in the region of Garanhuns, and M. bovis was detected by real-time PCR in one milk sample (0.25%) and eight blood samples (2%); the infected animals originated from 6 of the 20 properties sampled (30%) (CEZAR et al, 2016).…”

Section: Previous Studies Have Been Conducted In 12mentioning

confidence: 99%

Epidemiological situation of bovine tuberculosis in the State of Pernambuco, Brazil

Lima

Nascimento²,

Almeida³

et al. 2016

SCA

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The epidemiological situation of bovine tuberculosis was studied in the state of Pernambuco, Brazil. The state was divided into three regions, and a predetermined number of properties was randomly sampled in each region. In each property, females aged 24 months or older were randomly selected and subjected to the cervical comparative tuberculin test. Altogether, 5,728 animals from 906 properties were tested. In the evaluated properties, an epidemiological questionnaire was applied to identify risk factors related to bovine tuberculosis. The prevalence of infected herds and infected animals and in the state was 2.87% [1.82-4.50] and 0.62% [0.29-1.29], respectively. There was a predominance trend of infected herds in the properties located in the dry areas of Pernambuco, where most dairy properties are found. The risk factors related to the occurrence of positive herds were: presence of 18 or more cows in the herd, milking the cows two or three times a day, and sharing the grazing area. In conclusion, the prevalence of bovine tuberculosis in Pernambuco is low, and the best strategy to be adopted is the implementation of a surveillance system for detection and sanitation of infected herds, preferably by incorporating risk-based surveillance strategies. In addition, the State should implement an effective sanitary program to enable producers to test their animals for bovine tuberculosis before inserting them into the herds, as well as avoid sharing the grazing areas with properties that are not free of bovine tuberculosis. ResumoA situação epidemiológica da tuberculose bovina foi estudada no estado de Pernambuco. O estado foi dividido em três regiões e em cada uma delas foi aleatoriamente amostrado um número pré-estabelecido de propriedades. Dentro de cada propriedade, fêmeas com idade igual ou superior a 24 meses foram escolhidas aleatoriamente e submetidas ao teste Tuberculínico Cervical Comparativo. Ao todo foram testados 5.728 animais provenientes de 906 propriedades. Nas propriedades, foi aplicado um questionário epidemiológico para identificar fatores de risco associados à tuberculose bovina. A prevalência de focos no estado foi de 2,87% [1,82; 4,50] e a de animais 0,62% [0,29; 1,29]. Houve tendência de concentração de focos na região do Agreste do estado, caracterizada pelo predomínio de propriedades de leite. Os fatores de risco associados à condição de foco foram ter 18 ou mais vacas no rebanho, ordenhar as vacas 2 ou 3 vezes ao dia e compartilhar pastagem. Concluindo, o estado tem baixa prevalência de tuberculose bovina e a melhor estratégia a ser adotada é a implementação de sistema de vigilância para detecção e saneamento dos focos, de preferência incorporando elementos de vigilância baseada em risco. Além disso, o estado deve realizar uma sólida ação de educação sanitária para que seus produtores passem a testar os animais para tuberculose bovina antes de introduzi-los em seus plantéis e deixem de compartilhar pastagem com propriedades que não sejam livres de tuberculose bovina. Palavras-chave...

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“…700 Detection of Mycobacterium tuberculosis complex in cattle ________________________________________ Mycobacterium have been more frequently identified in blood samples of cattle compared to milk and other samples. Cezar et al (2016) reported detection of M. bovis from blood samples in two per cent of cattle maintained in 20 properties in Brazil. They suggested blood PCR as a highly sensitive method for detection of members of MTBC, compared to milk samples.…”

Section: Resultsmentioning

confidence: 99%

Molecular detection of Mycobacterium tuberculosis complex in bovine blood samples

Sarika¹,

Mani²,

Mini³

et al. 2022

Journal of Veterinary and Animal Sciences

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Bovine tuberculosis (bTB), predominantly caused by Mycobacterium bovis, is one of the most neglected zoonotic diseases of cattle. The lack of documented information on bTB is one of the most important hurdles in controlling the disease. The present study was carried out to detect the presence of Mycobacterium tuberculosis complex (MTBC) from blood samples of cattle presented for slaughter, using polymerase chain reaction. The study revealed presence of MTBC in seven out of 50 animals (fourteen per cent) with symptoms suggestive of bTB. The detection of the organism emphasises the need of further prevalence studies in Kerala, to implement proper control strategies.

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Molecular detection of Mycobacterium bovis in cattle herds of the state of Pernambuco, Brazil

Cited by 19 publications

References 27 publications

The clinical and serological diagnosis of Mycobacterium bovis in blood and milk serums of lactating cows by IDEXX ELISA test in Wasit and Dhi-Qar provinces/Iraq

The clinical and serological diagnosis of Mycobacterium bovis in blood and milk serums of lactating cows by IDEXX ELISA test in Wasit and Dhi-Qar provinces/Iraq

Epidemiological situation of bovine tuberculosis in the State of Pernambuco, Brazil

Molecular detection of Mycobacterium tuberculosis complex in bovine blood samples

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