Molecular detection of Mycoplasma wenyonii and ‘Candidatus Mycoplasma haemobos’ in cattle in Hokkaido, Japan

Tagawa, Michihito; Matsumoto, Kotaro; Inokuma, Hisashi

doi:10.1016/j.vetmic.2008.05.006

Cited by 87 publications

(77 citation statements)

References 12 publications

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“…In cattle, two distinct hemotropic Mycoplasma have been identified to date: Mycoplasma wenyonii (formerly Eperythrozoon wenyonii) (ADLER; ELLENBOGEN, 1934;SUTTON, et al, 1977) and 'Candidatus Mycoplasma haemobos' ('C. M. haemobos') (TAGAWA et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

“…'Candidatus Mycoplasma haemobos' has been reported using molecular methods such as polymerase chain reaction (PCR) and sequencing techniques in cattle from Switzerland, Germany, China, and Japan (HOFMANN-LEHMANN et al, 2004;TAGAWA et al, 2008;SU et al, 2010;HOELZLE et al, 2011). However, to the author's knowledge, no molecular detection has been reported to date in the Americas.…”

Section: Introductionmentioning

confidence: 99%

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Molecular detection and occurrence of 'Candidatus Mycoplasma haemobos' in dairy cattle of Southern Brazil

Girotto

Zangirólamo

Bogado

et al. 2012

Rev. Bras. Parasitol. Vet.

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Bovine hemoplasmas are bacteria found on the erythrocyte surface or free in the plasma of cattle. The aim of the present study was to evaluate the occurrence of 'Candidatus Mycoplasma haemobos' ('C. M. haemobos') in Holstein and Jersey cattle raised in Londrina and surroundings, northern region of the State of Parana, Southern Brazil. PCR testing directed to 16S rRNA gene fragment was performed to investigate the occurrence and characterize the molecular identity of 'C. M. haemobos'. A total of 264/433 (60.97%) blood samples were positive by PCR. Further alignment of 500-bp amplicons to available sequences at the GenBank database showed high identity (100%) to 'C. M. haemobos'. To the author's knowledge, this is the first molecular confirmation of the hemoplasma 'C. M. haemobos' in cattle from Brazil. Moreover, 'C. M. haemobos' was observed in high occurrence in dairy cattle, and may have significant impact in livestock production.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Molecular detection and occurrence of 'Candidatus Mycoplasma haemobos' in dairy cattle of Southern Brazil

Girotto

Zangirólamo

Bogado

et al. 2012

Rev. Bras. Parasitol. Vet.

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“…This novel bovine haemoplasma species clearly 1 differs from the classical bovine haemotrophic Mycoplasma wenyonii in its 16S rDNA 2 (identity of 81.2%); thus the provisional name "Candidatus Mycoplasma haemobos" was 3 suggested (Tagawa et al, 2008). Phylogenetically the novel bovine species "Candidatus M. 4…”

Section: Mycoplasmas 24mentioning

confidence: 92%

“…Mycoplasma haemofelis, and the canine Mycoplasma haemocanis species; it does not 6 cluster with M. wenyonii, the other bovine haemoplasma species from which the 16 rDNA 7 has yet been sequenced (Tagawa et al, 2008;Hoelzle et al, 2010). 8…”

Section: Mycoplasmas 24mentioning

confidence: 97%

‘Candidatus Mycoplasma haemobos’, a new bovine haemotrophic Mycoplasma species?

Hoelzle

Hofmann‐Lehmann

Hoelzle

2010

Veterinary Microbiology

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“…Various hemoplasmas have been detected in other animals [12,13,18], and pathogenic differences among them have been reported [2,3,14]. The phylogenetic analysis revealed that both 'Candidatus M. haemovis' and M. ovis belong to the same clade, which contains lower pathogenic species that infect other animals.…”

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confidence: 99%

A Clinical Case of Severe Anemia in a Sheep Coinfected with Mycoplasma ovis and Candidatus Mycoplasma haemovis' in Hokkaido, Japan

et al. 2012

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ABSTRACT. A 2-year-old East Friesian sheep imported from Australia exhibited severe anemia after contagious pustular dermatitis in Hokkaido, Japan. Hemoplasma infection was confirmed in blood smears. Both Mycoplasma ovis and 'Candidatus Mycoplasma haemovis' were detected by PCR and sequence analyses. In the epidemiological analysis, dual pathogens were detected in 6 of 12 (50.0%) sheep imported from Australia with the infected ewe at the same time, 1 of 5 (20.0%) sheep introduced from a domestic farm in Hokkaido, and in 1 of 16 (6.3%) sheep from an epidemiologically unrelated ranch. It is the first clinical case of sheep to confirm coinfection of these pathogens in Japan. Haemobartonella and Eperythrozoon cause infectious anemia in several mammalian species [8,17]. These pathogens have recently been reclassified as genus Mycoplasma on the basis of 16S rRNA gene analysis and morphological similarities [8]. Mycoplasma ovis (formerly Eperythrozoon ovis) causes ovine hemoplasmosis, which is characterized by poor weight gain, severe anemia, and even mortality in lambs and, occasionally, young adult sheep [1]. On the other hand, chronic sustained infection can occur asymptomatically in older sheep [4]. M. ovis also causes a more severe disease in goats [7]. Recently 'Candidatus Mycoplasma haemovis', a new ovine hemoplasma, has been detected in Hungary [5]. This is the first case report to describe M. ovis and 'Candidatus M. haemovis' coinfection in a sheep presenting with severe anemia in Japan.A 2-year-old East Friesian ewe imported from Australia in July 2009 showed contagious pustular dermatitis a month after import. Because anorexia continued after recovery, a blood test was carried out. Severe anemia with hematocrit of 14% was recorded. Erythrocytes with coccoid parasites on their surface were detected in a blood smear (Fig. 1), which strongly suggested infectious hemoplasma. An EDTA-anticoagulated blood sample was collected from the infected ewe. For the epidemiological study, blood was randomly collected from 12 sheep imported from Australia with the infected ewe at the same time, and were managed at a same sheep building until an investigation and, 5 sheep introduced from a domestic farm in Hokkaido. Additionally blood samples were also collected from 16 sheep kept in an epidemiologically unrelated ranch. It was geographically apart from the first ranch, and no movement of sheep has been recorded between the two ranches.Total DNA was extracted from 200 μl of blood using a QIAamp DNA Blood Mini Kit (QIAGEN, Hilden, Germany) and stored at -30ºC until use. For screening, PCR amplification was performed in a 25 μl reaction mixture containing 5 μl of each DNA template and an F2/R2 hemoplasma-specific primer set [6]. Longer 16S rRNA * CORRESPONDENCE TO: INOKUMA, H., Obihiro University of Agriculture and Veterinary Medicine, 080-8555 Obihiro, Japan. e-mail: inokuma@obihiro.ac.jp ©2012 The Japanese Society of Veterinary Science Fig. 1. Giemsa-stained blood smear from the anemic sheep with Mycoplasma ovis and 'Candidat...

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Molecular detection of Mycoplasma wenyonii and ‘Candidatus Mycoplasma haemobos’ in cattle in Hokkaido, Japan

Cited by 87 publications

References 12 publications

Molecular detection and occurrence of 'Candidatus Mycoplasma haemobos' in dairy cattle of Southern Brazil

Molecular detection and occurrence of 'Candidatus Mycoplasma haemobos' in dairy cattle of Southern Brazil

‘Candidatus Mycoplasma haemobos’, a new bovine haemotrophic Mycoplasma species?

A Clinical Case of Severe Anemia in a Sheep Coinfected with Mycoplasma ovis and Candidatus Mycoplasma haemovis' in Hokkaido, Japan

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