Molecular Detection of New Delhi Metallo Beta Lactamase 1 (NDM-1) Producing Bacterial Isolates in Kano- Northwestern Nigeria

Abdullahi, Sharif Alhassan; Arzai, AH; Ibrahim, Yusuf; Adamu, S; Koki, Y A; Rabi’u, M.A.; Abdollahi, Abbas

doi:10.9734/arrb/2017/33477

Cited by 7 publications

(7 citation statements)

References 3 publications

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“…The prevalence of MBL-producing Gram-negative bacteria in this study was found to be 39.3%. This result is higher than that reported by Oduyebo et al (2016) in Lagos (8.5%) and Abdullahi et al (2017) in Kano (8.6%). The highest MBL producers were Escherichia coli, Burkholderia cepacia, Klebsiella pneumoniae, Pseudomonas aeruginosa, Serratia fonticola and Proteus mirabilis.…”

Section: Discussioncontrasting

confidence: 70%

“…Metallo beta-lactamase-producing Gram-negative bacteria have been reported as important cause of nosocomial and community-acquired infections including urinary tract infections (UTIs), surgical wound infections, pyogenic infections, cystic fibrosis, septicemia, pneumonia, meningitis, bacteremia, endocarditis, osteomyelitis, pyelonephritis, enterocolotis, puerperal sepsis, peritonitis and soft tissue infection among others (Samuel et al, 2010;Chollom et al, 2012). The prevalence rates of these infections have been reported within and outside the country by many researchers: 11.23% by Ajuba et al (2020) in Anambra, 8.5% by Oduyebo et al (2016) in Lagos, 8.6% by Abdullahi et al (2017) in Kano and 36.1% by Adam and Elhag (2018) in Sudan. Thus, early detection of MBL producing organisms is of great importance to mitigate easy dissemination of infectious resistant phenotypes and also for prompt implementation of effective therapeutic control measures.…”

Section: Introductionmentioning

confidence: 99%

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Phenotypic detection of multi-drug resistant MBL-producing Gram-negative bacteria isolated from clinical samples of patients in hospitals in Akwa Ibom State, Nigeria

Etang¹,

Akpan²,

Inyang³

et al. 2023

wojast

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Metallo-beta-lactamase (MBL)-producing Gram-negative bacteria (GNB) continue to be a bane in the treatment of clinical infections in both community and hospital settings. Prompt detection of multidrug resistant (MDR) strains using antimicrobial susceptibility testing (AST) and MBL detection are vital for therapeutic options. The aim of this study was to determine the prevalence, distribution and antibiotic susceptibility of MDR and MBL-producing GNB from clinical samples in health facilities in Akwa Ibom State. A total of 480 samples comprising wound, urine and blood were collected aseptically from eligible in- and out-patients for the study and GNB were recovered from the samples using standard bacteriological techniques. The identification of isolated GNB, AST and detection of MBL-producing GNB were done using VITEK®2 COMPACT (Biomerieux) automated system, Modified Kirby Bauer disc diffusion method and IMP+EDTA CDT phenotypic method, respectively. Gram-negative bacterial growth was detected in 135 (77.1%.) cases with Escherichia coli (20.7%), Klebsiella pneumoniae (17.8%) and Burkholderia cepacia (14.1%) being the most preponderant isolates. Urine yielded more GNB, 45.2% than other samples. The isolates were sensitive to gentamicin (63%), imipenem (54.8%), and ofloxacin (46.7%) but showed high resistance to sulfamethoxazole-trimethoprim (78.5%), ceftriaxone (74.1%) and aztreonam (66.7%). The overall prevalence of MDR was 60% with the highest recorded in University of Uyo Teaching Hospital (UUTH), 64.8%. The overall prevalence of MBL producers was 39.3% with H. alvei, M. morgannii, P. mirabilis, R. radiobacter and P. aeruginosa being the majority, mostly from urine samples (47.5%) and UUTH health facility (43.7%). All MBL-producing GNB were MDR strains. Seven strains were pan-drug resistant. A combination of robust antibiotic and MBL screening of drug resistant GNB is essential for effective therapeutic decisions. Also, rational use of antibiotics, review of antibiotic usage policies and increased surveillance of MBL-producing GNB is strongly advocated.

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Section: Discussioncontrasting

confidence: 70%

Section: Introductionmentioning

confidence: 99%

Phenotypic detection of multi-drug resistant MBL-producing Gram-negative bacteria isolated from clinical samples of patients in hospitals in Akwa Ibom State, Nigeria

Etang¹,

Akpan²,

Inyang³

et al. 2023

wojast

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“…Carbapem-resistant bacteria such Escherichia coli and Klebsiella pneumoniae are mostly endemic in specific geographical regions, but reports of their spread into other geographical locations are point of grave concern these days. In Nigeria there have been reports of carbapenemase producing clinical isolates of enteric bacteria particularly among E. coli and Klebsiella species [1,15,16]. These strains become a serious threat to public health, associated with high mortality rates and have the potential to spread widely.…”

Section: Introductionmentioning

confidence: 99%

Prevalence and Antibiogram Profile of Carbapenem-resistant Escherichia coli and Klebsiella pneumoniae among Patients with Urinary Tract Infection in Abakaliki, Nigeria

Nomeh

Chukwu²,

Ogba³

et al. 2022

IJPR

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Background and Objectives: Carbapenem antibiotic are drug of last-resort from the treatment of bacterial infection, as a result of the prevalence and rapidly evolving enzymes from Carbapenem resistant bacteria such Escherichia coli and Klebsiella pneumoniae make urinary tract infection difficult, and in some cases impossible to treat in health care settings. With limited progress of new antibacterial drugs, the best approach is monitoring the prevalence and antibiogram profile of carbapenem-resistant Escherichia coli and Klebsiella pneumoniae among patients with UTI in Abakaliki, Nigeria. Methodology: A non-repetitive, clean catch mid-stream urine was collected from five hundred (500) diagnosed UTI inpatient and outpatient. The samples were evaluated using routine microbiological protocol for isolation and identification of Escherichia coli and Klebsiella pneumoniae. Phenotypic screening of Carbapenem-resistant strains was performed using Modified Hodge Testing. Antibiogram studies of carbapenem-resistant Escherichia coli and Klebsiella pneumoniae was performed using the Kirby–Bauer disk diffusion method and the results were interpreted using the Clinical Laboratory Standard Institute (CLSI) zone diameter breakpoints. Multiple antibiotic resistance index (MARI) was determined for MDR strain. Result: The prevalence of Escherichia coli and Klebsiella pneumoniae isolate accounted for 148(29.6 %) consisting of 95(54.3 %) and 53(16.3 %) from in-patients and out-patients. Escherichia coli accounted overall isolation rate of 112(22.4 %) comprising of high proportion among in-patient 82(46.9 %) over out-patient 30(9.2 %). The proportion of K. pneumoniae accounted for 36(7.2 %) with 13(7.4 %) and 23(7.1 %) recorded among in-patients and out-patients. Association between presence of Escherichia coli and Klebsiella pneumoniae isolates in clinical samples was statistically significant with patient’s population with p value <0.05. Carbapenem-resistant Escherichia coli and Klebsiella pneumoniae accounted for 37(7.4 %) comprising of 24(13.7) and 13(4.0 %) among in-patients and out-patients respectively while carbapenem-susceptible Escherichia coli and Klebsiella pneumoniae accounted for overall detection rate of 111(22.2 %) consisting of 71(40.6 %) and 40(12.3 %) among in-patients and out-patients respectively. The isolates resistance rate to cephalosporins were relatively high i.e., Cefotaxime, Cefoxtin Ceftazidime, Ceftriaxone resistance was observed at 60-100% while amoxicillin/clavulanate, azetronam, tetracycline nitrofurantoin and Ticarcillin-clavulanic acid recorded 100 % with MDR index ranged from 0.5-0.8, but were 100 % and 85.0 % sensitive to ciprofloxacin and ofloxacin. Conclusion: These results strongly hypothesize that MDR bacteria, including Carbapenem-resistant isolate, have become common residents in various hospital environments, however with substantial evidence in this study, ciprofloxacin and ofloxacin as drugs of choice could be used for treatment of UTI. Therefore, its importance that good antibiogram evaluation of other drug classes beside fluoroquinoles reported in this study need to be establishes as baseline for empirical diagnosis, epidemiological surveillance, drug prescriptions and infection management.

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“…[17][18][19][20][21] in South Africa, Kenya, Ethiopia and other eastern/southern African countries. In Nigeria, NDM-1 has been reported amongst Gram-negative enteric bacteria in various geopolitical zone, including the Southwestern region (Lagos), Northeastern region (Maiduguri), South-southern region (Bayelsa), North-western region (Kano), and South-eastern region (Enugu) of the country [22][23][24][25][26][27][28][29] however, non has reported NDM-1 amongst Gram-positive strains.…”

Section: Introductionmentioning

confidence: 99%

Occurrence of New Delhi Metallo-Beta-Lactamase 1 Producing Enterococcus Species in Oghara Water Nexus: An Emerging Environmental Implications of Resistance Dynamics

Esegbuyota

Onohuean

Oyama³

2022

Microbiol�Insights

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Various members of the enteric bacteria in recent times are evolving diverse survival mechanisms for antibiotic therapy resulting in failure of treatment in infection and disease cases. The Enterococcus species are potential strains implicated in gastrointestinal tract infection and are recently evolving in the resistance mechanism. The study evaluates the occurrence of New Delhi Metallo-beta-lactamase 1 (NDM-1) amongst Enterococcus species using the phenotypic and genomic characterization of environmental strains in the Oghara water nexus. Presumptive isolates of Enterococcus species were retrieved from various sampled water sources and confirmed using polymerase chain reaction (PCR). Antibiotic susceptibility testing was conducted on confirmed isolates using Kirby-Bauer disk diffusion methods. The result reveals 63 genus isolates confirmed Enterococcus species, of which 42 (67%) were Enterococcus faecium, 15 (23%) were Enterococcus faecalis, and 6 (10%) were other Enterococcus species. Fourteen among the E. faecalis isolates show resistance to Ertapenem-EDTA, while 17 (44.8%) of the E. faecium show resistance to Ertapenem-EDTA to presumptively reveal their NDM-1 phenotype. The PCR detection of the NDM-1 gene further confirmed 23 (36.5%) isolates as positive genotypes amongst the isolates that previously showed presumptive NDM-1 phenotype. It was also observed that 10 (15.9%) of Enterococcus faecium members harbored the NDM-1 genotype, whereas 8 (12.7%) members of the Enterococcus faecalis harbored the NDM-1 genotype. The observation of such resistance determinants necessitates a call for the adroit application of relevant therapeutics in the management of related infections and an environmental health caution to prevent the spread of such resistance potential enteric bacteria pathogens.

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Molecular Detection of New Delhi Metallo Beta Lactamase 1 (NDM-1) Producing Bacterial Isolates in Kano- Northwestern Nigeria

Cited by 7 publications

References 3 publications

Phenotypic detection of multi-drug resistant MBL-producing Gram-negative bacteria isolated from clinical samples of patients in hospitals in Akwa Ibom State, Nigeria

Phenotypic detection of multi-drug resistant MBL-producing Gram-negative bacteria isolated from clinical samples of patients in hospitals in Akwa Ibom State, Nigeria

Prevalence and Antibiogram Profile of Carbapenem-resistant Escherichia coli and Klebsiella pneumoniae among Patients with Urinary Tract Infection in Abakaliki, Nigeria

Occurrence of New Delhi Metallo-Beta-Lactamase 1 Producing Enterococcus Species in Oghara Water Nexus: An Emerging Environmental Implications of Resistance Dynamics

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