Molecular Detection of Pathogens in Seafood

Karunasagar, Iddya

doi:10.1002/9781444325546.ch23

Cited by 1 publication

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“…However, the microbial hygiene in such practice may underlie within the quality of food items consumed and the frequency of microbial shedding from the food handlers as well their style of handling, preparing and serving food [2][3][4][5]. Street vended foods such as poultry, beef, fish, water, juice and rice, which are sold in the street, are frequently identified as vehicles of disease transmission especially in the developing countries with high density of population [6][7][8][9]. Therefore, it is necessary to characterize the target microbial pathogen(s) in order to develop a suitable diagnostic test to detect them.…”

Section: Introductionmentioning

confidence: 99%

Use of Multiplex PCR Assay for Detection of Diarrheagenic Escherichia Coli in Street Vended Food Items

Sarker¹

2013

AJLS

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Pathogenic forms of Escherichia coli are commonly known to cause a variety of diarrheal diseases in hosts.Present study was designed to identify such pathogenic E. coli isolates from 7 different groups of street vended foods including cream, egg, non fried dry food and fried dry food, salad, cooked and non cooked items. Among 400 samples studied, almost all were found to be contaminated with an array of bacteria ranging between 3.24×10 2 -6.5×10 9 cfu/g. Results of multiplex polymerase chain reaction (PCR) assay revealed that out of 130 isolates of E. coli screened from all categories of food items, 52 (13%) were found to be enterotoxigenic E. coli, 32 (8%) were the Shiga toxin producing E. coli isolates, 14 (3.5%) were enteropathogenic and 6 (1.5%) were found to be enteroinvasive isolates. Results of plasmid profiling of the isolates was further in agreement to the presence of different pathogenic E. coli strains.

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Section: Introductionmentioning

confidence: 99%

Use of Multiplex PCR Assay for Detection of Diarrheagenic Escherichia Coli in Street Vended Food Items

Sarker¹

2013

AJLS

View full text Add to dashboard Cite

show abstract

Molecular Detection of Pathogens in Seafood

Cited by 1 publication

References 64 publications

Use of Multiplex PCR Assay for Detection of Diarrheagenic Escherichia Coli in Street Vended Food Items

Use of Multiplex PCR Assay for Detection of Diarrheagenic Escherichia Coli in Street Vended Food Items

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