Molecular Detection of Polymorphism of Heat Shock Protein 70 (Hsp70) in the Semen of Iraqi Holstein Bulls

Habib, Hassan Nima; Hassan, Amad Falah; Khudaier, Bassam Y.

doi:10.3923/ajas.2017.132.139

Cited by 7 publications

(2 citation statements)

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“…In goats, the gene coding for the HSP70 protein comprises 1926bp nucleotide sequences corresponding with 641 amino acids and is located on chromosome 23 (Gade et al, 2010). Although HSP70 genes are highly conserved molecules (Pawar et al, 2013), several studies suggest the possibility of genetic polymorphisms of the HSP70 gene [Fatima et al (2019) and Nikbin et al (2014) in goat; Habib et al (2017) in bulls; Habib et al (2018a) in rams; Habib (2020) in buffaloes and Habib et al (2020) in poultry]. Iraqi local goats generally suffer from many problems directly affecting their production.…”

Section: /9mentioning

confidence: 99%

Genetic profiling of HSP70 gene in local Iraqi goats

2022

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Animals display numerous physiological and behavioral responses that reduce the effects of heat stress. Moreover, genetic variance is strongly associated with responses to heat stress, including variants of heat shock proteins (HSPs) that are necessary for thermoregulation and stress resistance. Herein, we performed the molecular profiling of the HSP70 gene, and its polymorphism was demonstrated as a possible factor in the stress tolerance of local Iraqi goats. A number of different mutations were found owing to seven main polymorphisms. Results indicated the occurrence of silent and missense mutations in sequences obtained for Iraqi local goats. Genetic diversity was observed in the HSP70 gene of Iraqi local goats on the basis of phylogenetic-tree analysis as some mutations occurred once whereas others occurred multiple times. The polymorphisms LC616787, LC616788, and LC616791 were combined with the reference gene in the same branch, whereas polymorphisms (LC616785 and LC616786) and (LC616789 and LC616790) met in different branches, respectively. Moreover, all studied proteins had mismatches in their threedimensional structures. Therefore, the presence of specific genetic differences within the HSP70 gene in Iraqi goats can increase the possibility of selecting animals more suitable to various levels of stress.

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Section: /9mentioning

confidence: 99%

Genetic profiling of HSP70 gene in local Iraqi goats

2022

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“…Amplification performed in 25 μL reactions as mentioned by Habib (17) table 1, with forward primer 5'-ACAACCAGGTAGGTGAGCCA-3' and reverse primer 5'-TCCCAGTAGGCTGCATCTGA-3' (18) . The PCR cycling protocol was done as mentioned by Vega (19) as follows: initial stage (95 °C, 1min), then 35 amplification cycles (with denaturation at 95 °C, 30s), annealing (61 °C, 30s), extension (72 °C,1min), and final extension come after the last cycle (72 °C,7min).…”

Section: Pcr Amplificationmentioning

confidence: 99%

Genetic Profiling of CYP19 Gene Haplotype in Holstein Bulls

Hassan Nima Habib,

Khalaf A.H.Al-Rishdy,

Murthda F.AL-Hellou

2021

Indian Journal of Forensic Medicine & Toxicology

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The CYP19 gene is a protein-coding gene that stimulates the transmutation of androgens to estrogens, aromatase activity is related to haplotypes of the CYP19 gene, therefore this study aimed to detect haplotypes of the CYP19 gene in the Holstein bulls. This study, conducted from October 2018 to June 2019. Forty-two Bulls, aged between 4-6 years old were used, which belonged to local farmers in Basra city, south of Iraq. The DNA was extracted from semen, PCR amplification was carried out to acquire the DNA sequence. The sequences were analyzed by using some Bioinformatics tools. The results showed four haplotypes in the CYP19 gene as a result of silent and missense mutations in comparison with the same gene in GenBank, all the haplotypes are recorded in GenBank, DDBJ and EMBL. These findings call for further studies to reveal the relationship of CYP19 gene haplotypes and semen characteristics to uncover bulls' fertility.

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