Molecular detection of Rhipicephalus (Boophilus) annulatus resistance against deltamethrin in middle Egypt

Aboelhadid, Shawky M.; Arafa, Waleed M.; Mahrous, Lilian N.; Fahmy, Magdy M.; Kamel, Asmaa A.

doi:10.1016/j.vprsr.2018.06.008

Cited by 23 publications

(28 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Several reports have recorded ivermectin resistance in Rhipicephalus microplus populations in Mexico by Perez-Cogollo [12], Uruguay by Castro-Janer [13], and in Brazil by Klafke et al [6]. In a previous study, we showed that tick populations were resistant to deltamethrin in the same study areas (Beni-Suef, Egypt) [14]. Therefore, this study aimed to evaluate the resistance of field populations of Boophilus annulatus in Beni-Suef province, Egypt, to ivermectin.…”

Section: Introductionmentioning

confidence: 64%

First Report of Cattle Tick Rhipicephalus (Boophilus) annulatus in Egypt Resistant to Ivermectin

El‐Ashram

Aboelhadid

Kamel

et al. 2019

Insects

Self Cite

View full text Add to dashboard Cite

Tick control is mainly dependent on the application of acaricides, but resistance has developed to almost all classes of acaricides, including macrolactones. Therefore, we aimed to investigate ivermectin resistance among tick populations in middle Egypt. The larval immersion test was conducted using a commercial formulation of ivermectin (1%). Different concentrations of the immersion solution (0.0000625% (625 × 10−7%), 0.000125% (125 × 10−6%), 0.0005% (5 × 10−4%), 0.001% (1 × 10−3%), 0.0025% (2.5 × 10−3%), 0.005% (5 × 10−3), and 0.01% (1 × 10−2%)) were prepared by diluting a commercial ivermectin (1%) with distilled water containing 1% (v/v) ethanol and 2% (v/v) TritonX-100. Field populations of Rhipicephalus annulatus were collected from five different localities in Beni-Suef province, Egypt. Adult engorged female ticks were collected and assessed for oviposition and egg fertility. Eggs were collected, and hatched larvae were then used in the experiment. Application of acaricides was conducted on 10-day-old larvae. There was a significant difference in the LC50 (50% lethal concentration) among the examined localities on the log dose-response plot, where, the LC50 of tick populations from two localities (Emin elaros and Aldiabia) was higher than the other localities (Alhalabia, Alkom, and Beshnna). Besides, tick populations from Emin elaros and Aldiabia showed higher LC90 values with lower slope values compared to those from Alhalabia, Alkom, and Beshnna. According to these values (LC50, LC90, and slope values), as well as a history of acaricide failure to ticks in these areas, R. annulatus developed resistance to ivermectin. This study documents the first report of field populations of R. annulatus resistant to ivermectin in Egypt.

show abstract

Section: Introductionmentioning

confidence: 64%

First Report of Cattle Tick Rhipicephalus (Boophilus) annulatus in Egypt Resistant to Ivermectin

El‐Ashram

Aboelhadid

Kamel

et al. 2019

Insects

Self Cite

View full text Add to dashboard Cite

show abstract

“…Synthetic organic acaricides have been widely used to control ticks. The increasing resistance of ticks to these acaricides in addition to the problems associated with their meat and milk residues and potential toxicity on non-target organisms have become significant challenges to the effectiveness of such chemotherapy, however [ 30 , 31 ]. Accordingly, great efforts are now being devoted to investigate the natural products for acaricide activities, which probable to be a critical attributes and eco-friendly alternatives for ticks control [ 5 , 32 – 34 ].…”

Section: Discussionmentioning

confidence: 99%

Acaricidal activity of Foeniculum vulgare against Rhipicephalus annulatus is mainly dependent on its constituent from trans-anethone

et al. 2021

Self Cite

View full text Add to dashboard Cite

Globally, the economic losses due to hard ticks infestation and the control of the associated diseases have been calculated at USD $13.9–18.7 billion per year. The economic impact is related to its direct damage to the skins, blood loss, anemia, severe immunological reactions and indirect losses that related to the effects of hemoparasites, cost of treatment for clinical cases and expenses incurred in the control of ticks. The current study evaluated the acaricidal activities of fennel Foeniculum vulgare essential oil and its main components; trans-anethole and fenchone; against R. annulatus. GC–MS analysis revealed that this oil contained 16 components representing 99.9% of the total identified compounds with E-anethole being the predominant component(64.29%), followed by fenchone (9.94%). The fennel oil and trans-anethole showed significant acaricidal activities. The LC50 of the fennel oil was attained at concentrations of 12.96% for adult ticks and 1.75% for tick larvae meanwhile the LC50 of trans-anethole was reached at concentrations of 2.36% for adult tick and 0.56% for tick larvae. On the contrary, fenchone showed no any significant adulticidal activities and its LC50 attained at a concentration of 9.11% for tick larvae. Regarding repellence activities, trans-anethole achieved 100% repellency at the concentration of 10% while fennel showed 86% repellency at the same concentration. Fenchone showed no repellency effect. Treatment of larvae with fennel, trans-anethole, and fenchone LC50 concentrations significantly inhibited the acetylcholinesterase activity. Meanwhile, glutathione s-transferase activity was significantly decreased in fennel treated larvae but no significant effect was found in the larvae of trans-anethole and fenchone groups. These results indicate that the acaricide effect of fennel oil may attributed to its high content of trans-anethole. This was supported by potent adulticidal, larvicidal, and repellency effects of trans-anethole against Rhipeciphalus annulatus tick and therefore it could be included in the list of acaricide of plant origin.

show abstract

“…microplus include resistance to deltamethrin, where the use of the recommended dose (200 ppm) caused 33.33% mortality in ticks, and the application of double the recommended dose caused 56% mortality, while for the use of ivermectin, there are high resistance factors in treatments performed both in the laboratory and in the field. 50,51 These and other reports on ticks with resistance to different chemical acaricide formulations elucidate the difficulty that comes with the development of new molecules capable of exercising efficient control. 120…”

Section: Resistance To Chemical Controlmentioning

confidence: 95%

Background on the control of the cattle tick R. (B.) microplus and the use of coumarin substances as an alternative

Molano¹,

Torres²,

Monrroy³

2020

PPIJ

View full text Add to dashboard Cite

show abstract

Molecular detection of Rhipicephalus (Boophilus) annulatus resistance against deltamethrin in middle Egypt

Cited by 23 publications

References 41 publications

First Report of Cattle Tick Rhipicephalus (Boophilus) annulatus in Egypt Resistant to Ivermectin

First Report of Cattle Tick Rhipicephalus (Boophilus) annulatus in Egypt Resistant to Ivermectin

Acaricidal activity of Foeniculum vulgare against Rhipicephalus annulatus is mainly dependent on its constituent from trans-anethone

Background on the control of the cattle tick R. (B.) microplus and the use of coumarin substances as an alternative

Contact Info

Product

Resources

About