Molecular detection of the ruminal bacterium,<i>Butyrivibrio fibrisolvens</i>, in feces from rural residents of southern India

Balamurugan, Ramadass; Chittaranjan, Sucharita Priya; Chandragunasekaran, Aarthi Merlin; Ramakrishna, Balakrishnan S.

doi:10.3402/mehd.v21i1.7568

Cited by 3 publications

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“…Faecal bacteria were analysed by quantitative PCR using primers targeted at 16S ribosomal DNA of Clostridium coccoides–Eubacterium rectale group ( Clostridium cluster XIVa) ( 10 ) , Clostridium leptum group ( Clostridium cluster IV) ( 11 ) , Faecalibacterium prausnitzii ( 12 ) , Desulfovibrio species ( 12 ) , Bacteroides–Prevotella–Porphyromonas group ( 12 ) , Enterococcus faecalis ( 12 ) , Bifidobacterium genus ( 13 ) , Lactobacillus group ( 13 ) , Enterobacteria ( 14 ) , Ruminococcus productus–C. coccoides ( 15 ) , Butyrivibrio fibrisolvens ( 16 ) , and Roseburia spp.– E. rectale ( 17 ) . The latter are the principal butyrate-producing bacteria within Clostridium cluster XIVa.…”

Section: Methodsmentioning

confidence: 99%

Faecal microbiota composition in vegetarians: comparison with omnivores in a cohort of young women in southern India

Kabeerdoss

Devi

Mary³

et al. 2011

Br J Nutr

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The effect of vegetarian diets on faecal microbiota has been explored largely through culture-based techniques. The present study compared the faecal microbiota of vegetarian and omnivorous young women in southern India. Faecal samples were obtained from thirty-two lacto-vegetarian and twenty-four omnivorous young adult women from a similar social and economic background. Macronutrient intake and anthropometric data were collected. Faecal microbiota of interest was quantified by real-time PCR with SYBR Green using primers targeting 16S rRNA genes of groups, including: Clostridium coccoides group (Clostridium cluster XIVa), Roseburia spp. -Eubacterium rectale, Bacteroides -Prevotella group, Bifidobacterium genus, Lactobacillus group, Clostridium leptum group (Clostridium cluster IV), Faecalibacterium prausnitzii, Ruminococcus productus-C. coccoides, Butyrivibrio, Enterococcus species and Enterobacteriaceae. The groups were matched for age, socio-economic score and anthropometric indices. Intake of energy, complex carbohydrates and Ca were significantly higher in the omnivorous group. The faecal microbiota of the omnivorous group was enriched with Clostridium cluster XIVa bacteria, specifically Roseburia -E. rectale. The relative proportions of other microbial communities were similar in both groups. The butyryl-CoA CoA-transferase gene, associated with microbial butyrate production, was present in greater amounts in the faeces of omnivores, and the levels were highly correlated with Clostridium cluster XIVa and Roseburia-E. rectale abundance and to a lesser extent with Clostridium leptum and F. prausnitzii abundance and with crude fibre intake. Omnivores had an increased relative abundance of Clostridium cluster XIVa bacteria and butyryl-CoA CoA-transferase gene compared with vegetarians, but we were unable to identify the components of the diet responsible for this difference.

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Section: Methodsmentioning

confidence: 99%

Faecal microbiota composition in vegetarians: comparison with omnivores in a cohort of young women in southern India

Kabeerdoss

Devi

Mary³

et al. 2011

Br J Nutr

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“…Butyrivibrio fibrisolvens , a hemicellulolytic butyrate‐producing gut anaerobe, was shown to reduce aberrant crypt foci in experimental CRC. This organism is found widely in domestic ruminants and wild animals and has also been detected in human intestines in several populations in rural southern India, where lower rates of CRC have been observed …”

Section: Diet the Gut Microbiome And Colorectal Cancermentioning

confidence: 99%

Probiotic metabolites as epigenetic targets in the prevention of colon cancer

Nagpal²,

et al. 2012

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Dietary interventions for preventing colon cancer have recently attracted increased attention from researchers and clinicians. The probiotics have emerged as potential therapeutic agents but are also regarded as healthy dietary supplements for nutrition and health applications. The probiotic metabolome may interfere with various cellular and molecular processes, including the onset and progression of colon cancer. Probiotic metabolites may lead to the modulation of diverse cellular signal transduction and metabolic pathways. The gut microbial metabolites (organic acids, bacteriocins, peptides, etc.) have been noted to interact with multiple key targets in various metabolic pathways that regulate cellular proliferation, differentiation, apoptosis, inflammation, angiogenesis, and metastasis. Progress in this field suggests that epigenetic alterations will be widely used in the near future to manage colon cancer. The present review provides insights into the molecular basis of the therapeutic applications and the chemopreventive activities of certain probiotic metabolites, with emphasis on the interaction between these metabolites and the molecular signaling cascades that are considered to be epigenetic targets in preventing colon cancer.

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“…There are various studies that inconsistently reported that the F. prausnitzii abundance is associated with obesity [44][45][46] . In accordance with the report of gut microbiota composition in obese Indian children [46] , the abundance of F. prausnitzii significantly increased along with BMI increase in Iranian subjects. However, Feng et al [45] did not observe any significant difference of F. prausnitzii levels between obese and normal Chinese subjects.…”

Section: Discussionmentioning

confidence: 99%

The First Report of Differences in Gut Microbiota Composition between Obese and Normal Weight Iranian Subjects

Marvasti

Moshiri

Taghavi

et al. 2020

ibj

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Background: Obesity is a complex disorder influenced by various genetic and environmental factors. It has been shown that gut microbiota, which colonizes gastrointestinal tract, has a substantial role as an environmental factor in the pathophysiology of obesity. Since the composition of gut microbiota alters with regard to different criteria, such as ethnicity, geographical location, diet, lifestyle, age, and gender, we aimed to determine F/B ratio and the abundance of important gut microbiota members, A. muciniphila, F. prausnitzii, Roseburia, Bifidobacterium, and Prevotella in Iranian obese and normal weight individuals, for the first time. Methods: In this study, 50 normal and 50 obese subjects were recruited and classified based on their BMI into normal weight and obese groups. Stool samples were collected. Following DNA extraction from the samples, qPCR was conducted based on 16s rDNA universal primers. Finally, the correlation between the bacterial abundance and obesity was analyzed by statistical analyses. Results: We observed a significant increase of F/B ratio in the obese group, compared to the normal weight group (p = 0.002). Although A. muciniphila (p = 0.039) and Bifidobacterium (p = 0.049) abundance significantly decreased, the abundance of F. prausnitzii (p = 0.046) significantly elevated with BMI increase in the studied groups. Conclusion: Owing to the importance of the gut microbiota composition in obesity development, determination and targeted restoration of gut microbiota pattern could be valuable in the control and treatment of obesity in certain populations.

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Molecular detection of the ruminal bacterium,Butyrivibrio fibrisolvens, in feces from rural residents of southern India

Cited by 3 publications

References 0 publications

Faecal microbiota composition in vegetarians: comparison with omnivores in a cohort of young women in southern India

Faecal microbiota composition in vegetarians: comparison with omnivores in a cohort of young women in southern India

Probiotic metabolites as epigenetic targets in the prevention of colon cancer

The First Report of Differences in Gut Microbiota Composition between Obese and Normal Weight Iranian Subjects

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