Molecular detection of tick-borne haemopathogens in shelter dogs and Rhipicephalus sanguineus (sensu lato) ticks from Peninsular Malaysia

Sipin, Quincie; Kamal, Farina Mustaffa; Watanabe, Malaika; Rani, Puteri Azaziah Megat Abdul; Low, Van Lun; Aziz, Norazlina Abdul

doi:10.1016/j.cimid.2020.101563

Cited by 5 publications

(8 citation statements)

References 65 publications

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“…It was tested in 130 (36.1%) individuals, of which, E. canis and M. haemocanis constituted the most common co-infection pattern. Given that the co-infection of multiple vector-borne pathogens in the same canine is common in tropical areas [ 34 , 35 ], this might have been due to the high diversity of both infectious agents and vectors in such regions with poor access to veterinary care [ 16 ]. The occurrence of co-infection may cause greater pathogenicity whereby greater variable signs were exhibited by the affected dogs, resulting in a more challenging diagnosis.…”

Section: Discussionmentioning

confidence: 99%

Molecular Detection of Tick-Borne Pathogens in Stray Dogs and Rhipicephalus sanguineus sensu lato Ticks from Bangkok, Thailand

Phoosangwalthong

Kamyingkird

et al. 2021

Pathogens

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Canine tick-borne pathogens (CTBPs) such as Babesia vogeli, Ehrlichia canis, Anaplasma platys, Hepatozoon canis, and Mycoplasma haemocanis are important pathogens in dogs worldwide. Rhipicephalus sanguineus sensu lato, the main vector of several CTBPs, is the most common tick species found on dogs in Thailand. The present study identified CTBPs in dogs and ticks infested dogs. Samples (360 dog blood samples and 85 individual ticks) were collected from stray dogs residing in 37 temples from 24 districts in Bangkok and screened for CTBPs using molecular techniques. The most common CTBP found infecting dogs in this study was Ehrlichia canis (38.3%) followed by Mycoplasma haemocanis (34.2%), Hepatozoon canis (19.7%), Babesia vogeli (18.1%), and Anaplasma platys (13.9%), respectively. Furthermore, A. platys (22.4%) was the most common CTBP in ticks followed by M. haemocanis (18.8%), B. vogeli (9.4%), H. canis (5.9%), and E. canis (2.4%), respectively. The detection of CTBPs from the present study highlights the potential risk of infections that may occur in stray dogs and their ticks residing in Bangkok temples. These findings underline the importance of performing active surveys to understand the complexity of distributions of CTBPs in dogs and their ticks in Thailand.

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Section: Discussionmentioning

confidence: 99%

Molecular Detection of Tick-Borne Pathogens in Stray Dogs and Rhipicephalus sanguineus sensu lato Ticks from Bangkok, Thailand

Phoosangwalthong

Kamyingkird

et al. 2021

Pathogens

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“…Details of sample collection, tick identification, and DNA isolation were performed as described by Sipin et al (2020). Briefly, a total of 220 dog blood samples and 140 tick samples were collected from 10 animal shelters in North (Pulau Pinang, Kedah, Perak), South (Johor), East (Pahang), and Central (Selangor) regions of Peninsular Malaysia for the detection of VBHs.…”

Section: Parasites Isolationmentioning

confidence: 99%

“…Ticks can transmit a wide range of diseases, including viral, bacterial, and protozoan, to animal hosts (Otranto & Wall, 2008). Rhipicephalus sanguineus sensu lato (R. sanguineus), the brown dog tick, now known as Rhipicephalus linnaei (Šlapeta et al, 2021) is an important vector of canine vector-borne haemoparasites (VBHs) in Southeast Asia (SEA), and the primary tick infesting dogs in Malaysia (Dantas-Torres, 2010;Low et al, 2017;Nguyen et al, 2019;Sipin et al, 2020). The higher prevalence of VBHs in dogs and low prevalence in ticks were evident in previous studies (Galay et al, 2018;Low et al, 2018;Sipin et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

“…Rhipicephalus sanguineus sensu lato (R. sanguineus), the brown dog tick, now known as Rhipicephalus linnaei (Šlapeta et al, 2021) is an important vector of canine vector-borne haemoparasites (VBHs) in Southeast Asia (SEA), and the primary tick infesting dogs in Malaysia (Dantas-Torres, 2010;Low et al, 2017;Nguyen et al, 2019;Sipin et al, 2020). The higher prevalence of VBHs in dogs and low prevalence in ticks were evident in previous studies (Galay et al, 2018;Low et al, 2018;Sipin et al, 2020). Nevertheless, stray dogs were more susceptible to VBHs infection compared to pet dogs due to the high exposure to arthropod vectors (Cao et al, 2015).…”

Section: Introductionmentioning

confidence: 99%

“…Several studies in Malaysia have started focusing on phylogenetic analysis of VBHs in dogs and their arthropod vector, tick (Low et al, 2018;Sipin et al, 2020). Nonetheless, further studies are required on the correlation of the presence of VBHs in dogs with the arthropod vector to investigate the origin of the infection.…”

Section: Introductionmentioning

confidence: 99%

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Low Genetic Diversity of Vector-Borne Haemoparasites in Dogs and Their Ticks Revealed Local and Long-Range Transmission in Peninsular Malaysia

Sipin¹,

Kamal²,

Watanabe³

et al. 2023

JTAS

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Molecular methods coupled with phylogenetic analysis are sensitive tools for detecting and classifying parasites. This study used nuclear and mitochondrial gene markers to investigate the host-vector interaction of the vector-borne haemoparasites. The population genetic structures of important vector-borne haemoparasites in dogs, namely, Anaplasma platys, Ehrlichia canis, Babesia vogeli, and Babesia gibsoni, were determined from the nuclear gene of 16S or 18S rRNA gene, gltA and groESL and mitochondrial gene of COX1 across dogs and vector ticks. A total of 220 blood samples and 140 ticks were collected from shelter dogs in Peninsular Malaysia. Out of the positive samples for the vector-borne haemoparasites, 28 positive blood isolates and six tick isolates were selected and characterised. There was a low diversity in tick sequences, while varying degree of variability was observed in dogs’ sequences. Overlapped haplotypes were observed in sequences of dogs and ticks, revealing the possibility of the same infection origin. No regional separation was detected, but similar haplotypes from different regions were observed. These findings contribute to the epidemiology of vector-borne haemoparasites in dogs in Malaysia.

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Ticks analysis for molecular detection and phylogenetic evaluation of stray dogs infecting protozoa from Alborz, Iran

Rivaz,

Nasiri,

Taiefi Nasrabadi

et al. 2024

Microbial Pathogenesis

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Molecular detection of tick-borne haemopathogens in shelter dogs and Rhipicephalus sanguineus (sensu lato) ticks from Peninsular Malaysia

Cited by 5 publications

References 65 publications

Molecular Detection of Tick-Borne Pathogens in Stray Dogs and Rhipicephalus sanguineus sensu lato Ticks from Bangkok, Thailand

Molecular Detection of Tick-Borne Pathogens in Stray Dogs and Rhipicephalus sanguineus sensu lato Ticks from Bangkok, Thailand

Low Genetic Diversity of Vector-Borne Haemoparasites in Dogs and Their Ticks Revealed Local and Long-Range Transmission in Peninsular Malaysia

Ticks analysis for molecular detection and phylogenetic evaluation of stray dogs infecting protozoa from Alborz, Iran

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