Molecular detection of Toxoplasma gondii among commensal rodents from the Sahiwal division, Punjab, Pakistan

Rizwan, Muhammad; Ali, Shahzad; Javid, Arshad; Rashid, Muhammad Imran

doi:10.1007/s00436-022-07729-8

Cited by 6 publications

(6 citation statements)

References 47 publications

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“…In a previous study [8], rodent sera was also tested using the serological method (a bespoke ELISA test), and therefore in this case, the results of serological tests were compared with molecular tests. In small rodents, T. gondii tissue cysts (bradyzoites) are mainly located in the brain [41,42]. Based on this knowledge, the brains, spleens and blood of seven rodent species were used in this study to detect T. gondii DNA.…”

Section: Discussionmentioning

confidence: 99%

Identification of Toxoplasma gondii in wild rodents in Poland by molecular and serological techniques

Nowicka,

Antolova,

Lass

et al. 2024

Ann Agric Environ Med.

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Rodents are recognized as reservoirs for Toxoplasma gondii, playing a crucial role in maintaining the parasite's presence in the environment. Biomonitoring was conducted to assess the role of sylvatic rodents in maintaining T. gondii, and to analyse the prevalence and seroprevalence of the parasite in seven wild rodent species. Rodents were collected in an open grassland study site located in northeastern Poland, and dissected. Brain, spleen, blood and serum samples were collected. Molecular (PCR assay, nested-PCR assay) and serological (ELISA and agglutination tests) methods were applied to indicate the best approach for application in the biomonitoring of T. gondii in small mammals. Samples were screened from 68 individuals using PCR assays but no T. gondii DNA were found. The agglutination test showed no signal. Antibodies against T. gondii were found in 5 sera samples out of 56 analysed (seroprevalence = 8. 9% [4.4-16.8]). The results confirmed that rodents participate in the life cycle of T. gondii as reservoirs of this parasite in the sylvatic environment. However, for effective bio-monitoring of T. gondii in small mammals, the results suggest a preference for utilizing ELISA tests to detect T. gondii antigens, as opposed to relying solely on molecular methods.

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Section: Discussionmentioning

confidence: 99%

Identification of Toxoplasma gondii in wild rodents in Poland by molecular and serological techniques

Nowicka,

Antolova,

Lass

et al. 2024

Ann Agric Environ Med.

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show abstract

“…For example Ivovic et al (2019), found that on the Istrian peninsula T. gondii was rarely detectable among species of small rodents such as A. sylvaticus, A. flavicollis and A. agrarius [37]. Recent studies by Rizwan et al (2023) based on molecular detection of T. gondii via amplification of the B1 and the SAG3 genes by PCR and nested-PCR assays showed that only 14 (5.9%) of 236 rodents trapped in Pakistan showed signs of the presence of T. gondii DNA [41]. This is in line with results from rats tested at a mink farm in China.…”

Section: Discussionmentioning

confidence: 99%

“…Our study suggests that rodents living in Urwitałt in the Mazury Lake District in Northeastern Poland are exposed to T. gondii, posing a potential public health risk to local residents and visitors to the region. Considering other recent reports [41,43,51] based on molecular and serological techniques, further monitoring is needed to fully understand the status of T. gondii in the wild and to assess comprehensively the risk of transmission of this pathogen to the human communites in the region [5].…”

Section: Discussionmentioning

confidence: 99%

Detection of Toxoplasma gondii in Sylvatic Rodents in Poland Using Molecular and Serological Methods

Nowicka¹,

Antolová²,

Lass³

et al. 2023

Preprint

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Rodents are known to be reservoirs of Toxoplasma gondii and keep the parasite circulation in the environment. We conducted biomonitoring to assess the role of sylvatic rodents in maintaining T. gondii and to analyse the prevalence and seroprevalence of the parasite in seven wild rodent species. Rodents were collected in our study sites (woodland and open grasslands) located in northeastern Poland and dissected. We collected brain, spleen, blood and serum samples. We applied both molecular (PCR assay, nested-PCR assay) and serological (ELISA and agglutination tests) methods to indicate the best approach for application in the biomonitoring of T. gondii in small mammals. We screened samples from 95 individuals sing PCR assays and found no T. gondii DNA. The agglutination test showed no signal. We found antibodies against T. gondii in 6 sera samples out of 74 analysed (seroprevalence = 8.11% [4.0-17.1]). Our results confirm that rodents participate in the life cycle of T. gondii as reservoirs of this parasite in the sylvatic environment. However, biomonitoring should be performed with the ELISA tests to search for T. gondii antigens, rather than a molecular approach only.

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“…For example Ivovic et al (2019), found that on the Istrian peninsula T. gondii was rarely detectable among species of small rodents such as A. sylvaticus, A. avicollis and A. agrarius [37]. Recent studies by Rizwan et al (2023) based on molecular detection of T. gondii via ampli cation of the B1 and the SAG3 genes by PCR and nested-PCR assays showed that only 14 (5.9%) of 236 rodents trapped in Pakistan showed signs of the presence of T. gondii DNA [41]. This is in line with results from rats tested at a mink farm in China.…”

Section: Discussionmentioning

confidence: 99%

“…Our study suggests that rodents living in Urwitałt in the Mazury Lake District in Northeastern Poland are exposed to T. gondii, posing a potential public health risk to local residents and visitors to the region. Considering other recent reports [41,43,50] based on molecular and serological techniques, further monitoring is needed to fully understand the status of T. gondii in the wild and to assess comprehensively the risk of transmission of this pathogen to the human communites in the region [5].…”

Section: Discussionmentioning

confidence: 99%

Detection of Toxoplasma gondii in sylvatic rodents in Poland using molecular and serological methods

Nowicka

Antolová

Lass

et al. 2023

Preprint

View full text Add to dashboard Cite

Rodents are known to be reservoirs of Toxoplasma gondii and keep the parasite circula-tion in the environment. We conducted biomonitoring to assess the role of sylvatic rodents in maintaining T. gondii and to analyse the prevalence and seroprevalence of the parasite in seven wild rodent species. Rodents were collected in our open grassland study site located in north-eastern Poland and dissected. We collected brain, spleen, blood and serum samples. We applied both molecular (PCR assay, nested-PCR assay) and serological (ELISA and agglutination tests) methods to indicate the best approach for application in the biomonitoring of T. gondii in small mammals. We screened samples from 68 individuals sing PCR assays and found no T. gondii DNA. The agglutination test showed no signal. We found antibodies against T. gondii in 5 sera samples out of 56 analysed (seroprevalence = 8.9% [4.4–16.8]). Our results confirm that rodents participate in the life cycle of T. gondii as reservoirs of this parasite in the sylvatic environment. However, biomonitoring should be performed with the ELISA tests to search for T. gondii anti-gens, rather than a molecular approach only.

show abstract

Molecular detection of Toxoplasma gondii among commensal rodents from the Sahiwal division, Punjab, Pakistan

Cited by 6 publications

References 47 publications

Identification of <i>Toxoplasma gondii</i> in wild rodents in Poland by molecular and serological techniques

Identification of <i>Toxoplasma gondii</i> in wild rodents in Poland by molecular and serological techniques

Detection of <em>Toxoplasma gondii</em> in Sylvatic Rodents in Poland Using Molecular and Serological Methods

Detection of Toxoplasma gondii in sylvatic rodents in Poland using molecular and serological methods

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