Molecular detection of β-lactamase genes in Klebsiella pneumoniae and Escherichia coli isolated from different clinical sources

Abstract

The rising occurrence of infections generated by Escherichia coli and Klebsiella pneumoniae that produce extended-spectrum β-lactamase (ESBL) is reason for concern. Due to the recent emergence of multidrug-resistant microorganisms that develop ESBL. The purpose of this work was to detect the ESBLs in clinical isolates of E. coli and K. pneumoniae. 118 samples of E. coli and 63 isolates of K. pneumoniae were collected from clinical samples. Polymerase chain reaction was used to detect β-lactamase genes (i.e., blaTEM, blaSHV, and blaCTX-M). Phenotypic detection revealed that 48.31% and 85.19% of E. coli and K. pneumoniae produced ESBLs, respectively. Whereas screening of ESBL genes in both bacteria employing a multiplex PCR test revealed that 24.58% of the ESBL-producing E. coli strains contained blaTEM, 50.85% contained blaSHV, and 32.2% contained blaCTX-M. Nevertheless, in K. pneumoniae, 40.74% blaTEM, 35.19% blaSHV, and 64.81% blaCTX-M genes were present. Antimicrobial resistance profiles of E. coli and K. pneumoniae isolates to twenty antibiotics were observed to vary significantly. Additionally, it was determined that the majority of E. coli and K. pneumoniae isolates were multidrug resistant (MDR). Additionally, 80.51% of E. coli isolates were resistant to the AMC antibiotic, while 0.00% were resistant to IPM and MEM. From the other hand, the resistant proportion of K. pneumoniae isolates was heterogeneous, ranging from 69.84% against CAZ to 0.00% against CIP and G antibiotics. The blaSHV gene was the most widespread among different forms of ESBLs in E. coli, but the most common gene in K. pneumoniae isolates was blaCTX-M (64.81%).

Behavioral and Biochemical Variations in Unio tigridis After Exposure to Lead Nitrate

“…The GeneAll® ExgeneTM for Clinic Cell SV small kit was used to separate and purify the genomic DNA from the adductor muscle (Songpa-gu, Seoul, Korea). The amount of genomic DNA was extracted before PCR was determined by agar gel electrophoresis [15]. To ascertain the quantity and quality of DNA, the Nano Drop 1000 spectrophotometer was equipped to detect the optical density of a DNA sample as well as the concentration and purity of genomic DNA extraction.…”

Molecular detection of blaTEM, blaSHV,and blaCTX-M genes among Uropathogenic Escherichia coli isolated from cases with urinary tract infection in Erbil city-Iraq

“…Before the PCR run, agarose gel electrophoresis was used to evaluate the extracted genomic DNA in E. coli. 1.5% agarose gel was used and ran on 85V for 45 min [12].…”

Acute Toxicity of Chlorpyrifos on the Freshwater Bivalves (Unio Tigridis) and Effects on Bioindicators

“…Genomic DNA from adductor muscle was isolated and purified using the GeneAll® ExgeneTM for Clinic Cell SV small kit (Songpa-gu, Seoul, Korea). Agar gel electrophoresis was used to gauge the amount of genomic DNA that had been extracted before PCR 19 . The Nano Drop 1000 spectrophotometer is prepared to measure the optical density of a DNA sample as well as the concentration and purity of genomic DNA extraction to determine the amount and purity of DNA.…”