Molecular detections of coronavirus: current and emerging methodologies

Rapid and Visual Detection of SARS-CoV-2 RNA Based on Reverse Transcription-Recombinase Polymerase Amplification with Closed Vertical Flow Visualization Strip Assay

“…Digital PCR is an absolute measurement method that combines limited dilution, endpoint PCR, and Poisson statistics ( 18 ). It has a lower false-negative rate than RT-PCR but is more time-consuming and more expensive than RT-PCR ( 19 , 20 ). Additional novel biochemical methods are also under development.…”

Lab-on-a-chip for the easy and visual detection of SARS-CoV-2 in saliva based on sensory polymers

“…PCRs belong to the group of nuclear acid-based detection methods, and antigen tests to the group of protein-based detection methods [6] . On the one hand, nuclear acid-based detection methods, such as PCR or Loop-Mediated Isothermal Amplification (LAMP), require an amplification process in which a few DNA strands can be replicated to produce a much larger sample.…”

“…One of the most promising techniques for detecting these types of viruses is a subgroup of the protein-based detection methods called protein function-based detection methods [6] , [10] , [11] , [12] , [13] . They are based on using a peptide substrate to detect an enzyme closely related to the coronavirus, in this case, the Mpro protein [6] .…”

“…One of the most promising techniques for detecting these types of viruses is a subgroup of the protein-based detection methods called protein function-based detection methods [6] , [10] , [11] , [12] , [13] . They are based on using a peptide substrate to detect an enzyme closely related to the coronavirus, in this case, the Mpro protein [6] . Mpro (also called 3CLpro), is a cysteine hydrolase-like protease essential in viral replication that cleavages polyproteins and generates the corresponding functional proteins necessary for virion formation [14] .…”

Nanopore sensors for viral particle quantification: current progress and future prospects

“…Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has been rapidly spreading worldwide since December 2019, when it first was discovered in Hubei, China [ 2 ]. This pandemic once again pointed out the need for developing versatile point-of-need (PoN) monitoring and point-of-care (PoC) diagnostic devices for performing accurate, fast, cost-effective, and laboratory-free tests upon the emergence of a new class of pathogen [ 3 , 4 ]. In this paper, PoN devices refer to the ones used to monitor non-patient samples such as air and water, while PoC devices refer to technologies used to test human body fluids.…”