Molecular Determination of mirRNA-126 rs4636297, Phosphoinositide-3-Kinase Regulatory Subunit 1-Gene Variability rs7713645, rs706713 (Tyr73Tyr), rs3730089 (Met326Ile) and Their Association with Susceptibility to T2D

Mir, Rashid; Elfaki, Imadeldin; Duhier, Faisel M Abu; Alotaibi, Maeidh A.; Alalawy, Adel I.; Barnawi, Jameel; Babakr, Abdullatif Taha; Mir, Mohammad Muzaffar; Mirghani, Hyder; Hamadi, Abdullah; Dabla, Pradeep Kumar

doi:10.3390/jpm11090861

Cited by 10 publications

(6 citation statements)

References 44 publications

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“…T2DM is the result of the combined effects of genetic background, gender, aging, lifestyle, obesity and other factors. 6 , 7 , 26 Age and gender differences in the risk, onset, and progress of T2DM were found in previous studies. 27 , 28 When stratified by gender and age, rs3825199, rs11107116, and rs10492321 were conferred to an increased T2DM risk among males, and among the subjects aged ≤60 years ( Suppl_Table 3 ).…”

Section: Discussionsupporting

confidence: 52%

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The Effect of SOCS2 Polymorphisms on Type 2 Diabetes Mellitus Susceptibility and Diabetic Complications in the Chinese Han Population

Pan¹,

Tong²,

Deng³

et al. 2022

PGPM

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Background SOCS2 is downregulated in diabetes, which might be related to diabetes. We explored the effect of SOCS2 polymorphisms on the development of type 2 diabetes mellitus (T2DM) and diabetic complications. Methods The subjects consisted of 500 patients with T2DM and 501 healthy controls. Five variants in SOCS2 were genotyped by Agena MassARRAY system. RT-qPCR profiling was performed to detect the expression of SOCS2 mRNA. Logistic regression analysis was utilized to calculate odds ratio (OR) and 95% confidence intervals (95% CIs). Results Rs3825199 (OR = 1.44, p = 0.007), rs11107116 (OR = 1.39, p = 0.014) and rs10492321 (OR = 1.48, p = 0.004) had an increased T2DM risk of T2DM. Moreover, the contribution of SOCS2 polymorphisms to T2DM risk was associated with age, gender, smoking, drinking, and BMI. SOCS2 variants also had a reduced risk for T2DM patients with diabetic nephropathy, diabetic retinopathy and coronary heart disease. SOCS2 rs10492321 was the best single locus model. SOCS2 mRNA was downregulated in patients with T2DM compared to healthy controls ( p = 0.029). Conclusion This study firstly reported that rs3825199, rs11107116 and rs10492321 in SOCS2 conferred to an increased risk for the occurrence of T2DM in the Chinese Han population. Moreover, SOCS2 mRNA was downregulated in patients with T2DM, suggesting that SOCS2 might have an important role in the occurrence of T2DM.

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Section: Discussionsupporting

confidence: 52%

“… 5 In addition, genetic factors are strongly contributed to the etiology and manifestation of T2DM. 6 , 7 To date, a variety of risk loci affecting T2DM susceptibility have been recognized, 8–10 but numerous loci remain to be detected.…”

Section: Introductionmentioning

confidence: 99%

The Effect of SOCS2 Polymorphisms on Type 2 Diabetes Mellitus Susceptibility and Diabetic Complications in the Chinese Han Population

Pan¹,

Tong²,

Deng³

et al. 2022

PGPM

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“…They have been found to have an effect on glucose metabolism. For example, a recent study showed that miR-126 single nucleotide polymorphism was associated with T2DM[ 101 ]. MiRNAs may be a target therapeutic strategy in the future.…”

Section: Discussionmentioning

confidence: 99%

Role of antidiabetic agents in type 2 diabetes patients with chronic kidney disease

Lin¹,

Liu²,

Ling³

et al. 2023

World J Diabetes

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Insulin resistance is a condition in which the target tissues have a decreased response to insulin signaling, resulting in glucose uptake defect, and an increased blood sugar level. Pancreatic beta cells thus enhance insulin production to compensate. This situation may cause further beta cell dysfunction and failure, which can lead diabetes mellitus (DM). Insulin resistance is thus an important cause of the development of type 2 DM. Insulin resistance has also been found to have a strong relationship with cardiovascular disease and is common in chronic kidney disease (CKD) patients. The mechanisms of insulin resistance in CKD are complex and multifactorial. They include physical inactivity, inflammation and oxidative stress, metabolic acidosis, vitamin D deficiency, adipose tissue dysfunction, uremic toxins, and renin-angiotensin-aldosterone system activation. Currently, available anti-diabetic agents, such as biguanides, sulfonylureas, thiazolidinediones, alfa-glucosidase inhibitors, glucagon-like peptide-1-based agents, and sodium-glucose co-transporter-2 inhibitors, have different effects on insulin resistance. In this short review, we describe the potential mechanisms of insulin resistance in CKD patients. We also review the interaction of currently available anti-diabetic medications with insulin resistance.

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“…Previously, β-thalassemia prenatally was diagnosed by successfully using ARMS-PCR system in China and in Iran (Fu et al, and Moghadam et al), respectively [ 26 , 27 ]; Chiu et al, utilized ARMS-PCR to detect the wild as well as mutant mitochondrial tDNA heteroplasmies [ 28 ], whereas ARMS-PCR was applied to the antenatal diagnosis of cystic fibrosis [ 29 ]. Rashid Mir et al, applied ARMS-PCR for studying genotyping in coronary artery disease, breast cancer and also utilized ARMS-PCR for microRNA genotyping [ 30 , 31 , 32 , 33 ]. Recently, Singh et al [ 34 ] utilized ARMS PCR for prenatal diagnosis for SCD.…”

Section: Discussionmentioning

confidence: 99%

Molecular Determination of Vascular Endothelial Growth Factor, miRNA-423 Gene Abnormalities by Utilizing ARMS-PCR and Their Association with Fetal Hemoglobin Expression in the Patients with Sickle Cell Disease

Hamadi

Mir

Mahzari

et al. 2022

CIMB

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Recent studies have indicated that microRNA and VEGF are considered to be genetic modifiers and are associated with elevated levels of fetal haemoglobin HbF, and thus they reduce the clinical impact of sickle haemoglobin (HbS) patients. This cross-sectional study was performed on clinical confirmed subjects of SCD cases. miR-423-rs6505162 C>T and VEGF-2578 C>A genotyping was conducted by ARMS-PCR in SCD and healthy controls. A strong clinical significance was reported while comparing the association of miR-423 C>T genotypes between SCD patients and controls (p = 0.031). The microRNA-423 AA genotype was associated with an increased severity of SCD in codominant model with odd ratio (OR = 2.36, 95% CI, (1.15–4.84), p = 0.018) and similarly a significant association was observed in recessive inheritance model for microRNA-423 AA vs (CC+CA) genotypes (OR = 2.19, 95% CI, (1.32–3.62), p < 0.002). The A allele was associated with SCD severity (OR = 1.57, 95% CI, (1.13–2.19), p < 0.007). The distribution of VEGF-2578 C>A genotypes between SCD patients and healthy controls was significant (p < 0.013). Our results indicated that in the codominant model, the VEGF-2578-CA genotype was strongly associated with increased SCD severity with OR = 2.56, 95% CI, (1.36–4.82), p < 0.003. The higher expression of HbA1 (65.9%), HbA2 (4.40%), was reported in SCD patients carrying miR-423-AA genotype than miR-423 CA genotype in SCD patients carrying miR-423 CA genotype HbA1 (59.98%), HbA2 (3.74%) whereas SCD patients carrying miR-423 CA genotype has higher expression of HbF (0.98%) and HbS (38.1%) than in the patients carrying AA genotype HbF (0.60%), HbS (36.1%). ARMS-PCR has been proven to be rapid, inexpensive and is highly applicable to gene mutation screening in laboratories and clinical practices. This research highlights the significance of elucidating genetic determinants that play roles in the amelioration of the HbF levels that is used as an indicator of severity of clinical complications of the monogenic disease. Further well-designed studies with larger sample sizes are necessary to confirm our findings.

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Molecular Determination of mirRNA-126 rs4636297, Phosphoinositide-3-Kinase Regulatory Subunit 1-Gene Variability rs7713645, rs706713 (Tyr73Tyr), rs3730089 (Met326Ile) and Their Association with Susceptibility to T2D

Cited by 10 publications

References 44 publications

The Effect of SOCS2 Polymorphisms on Type 2 Diabetes Mellitus Susceptibility and Diabetic Complications in the Chinese Han Population

The Effect of SOCS2 Polymorphisms on Type 2 Diabetes Mellitus Susceptibility and Diabetic Complications in the Chinese Han Population

Role of antidiabetic agents in type 2 diabetes patients with chronic kidney disease

Molecular Determination of Vascular Endothelial Growth Factor, miRNA-423 Gene Abnormalities by Utilizing ARMS-PCR and Their Association with Fetal Hemoglobin Expression in the Patients with Sickle Cell Disease

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