Molecular diagnosis of a laboratory mouse papillomavirus (MusPV)

Joh, Joongho; Jenson, A. Bennett; Proctor, Mary; Ingle, Arvind; Silva, Kathleen A.; Potter, Christopher S.; Sundberg, John P.; Ghim, Shin-je

doi:10.1016/j.yexmp.2012.07.001

Cited by 35 publications

(49 citation statements)

References 28 publications

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“…Previous studies have indicated that inoculation of the muzzles of immunosuppressed mice with MusPV1 can induce papillomas (13,14). In addition to confirming this observation, we demonstrated that cell extracts of these papillomas can undergo at least 10 serial passages with no loss of biological potency, and we confirmed that infection is virion dependent, since it was prevented by passive transfer of an immune serum raised against the MusPV1 major capsid protein, L1.…”

Section: Discussionsupporting

confidence: 86%

“…Indeed, we are unaware of a PV other than MusPV1 in which L1 is reported to be found in the cytoplasm. It may be noted that this distribution of L1 may appear to differ from what has been described previously for MusPV1-induced lesions, where PV-specific antigens were found in the nuclei of cells in the stratum granulosum in the spontaneous papilloma found in the original immunodeficient mouse colony (13) and in papillomas experimentally induced by MusPV1 (14). However, those studies used rabbit polyclonal antibodies raised against disrupted virions of canine cutaneous PV type 2 for the immunohistochemical staining, so it is not clear whether they recognize MusPV1 L1, L2, or both proteins.…”

Section: Discussioncontrasting

confidence: 64%

“…A recent report has confirmed the ability of the MusPV1 genome to induce papillomas in Foxn1 nu /Foxn1 nu mice (15). The presence of large arrays of virus particles in lesional keratinocytes (13,14), together with the recent identification of an MusPV1 variant, displaying 99% nucleotide identity to the original MusPV1, in normal skin from a German house mouse (8), supports the conclusion that MusPV1 is a bona fide domestic mouse virus.…”

mentioning

confidence: 70%

“…The virus was identified in an immunodeficient, inbred NMRI-Foxn1 nu /Foxn1 nu nude mouse colony and was termed MusPV1. In the infected colony, mice spontaneously developed papillomas at cutaneous surfaces near the mucocutaneous junctions of nose and mouth, and infectious MusPV1 virions isolated from these lesions induced papilloma formation on the muzzles of immunodeficient B6.Cg-Foxn1 nu / Foxn1 nu mice (14). The papillomas showed typical histologic features of PV-associated lesions and expressed PV group-specific antigens.…”

mentioning

confidence: 99%

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Characterization of Mus musculus Papillomavirus 1 Infection In Situ Reveals an Unusual Pattern of Late Gene Expression and Capsid Protein Localization

Handisurya

Day

Thompson

et al. 2013

J Virol

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Full-length genomic DNA of the recently identified laboratory mouse papillomavirus 1 (MusPV1) was synthesized in vitro and was used to establish and characterize a mouse model of papillomavirus pathobiology. MusPV1 DNA, whether naked or encapsidated by MusPV1 or human papillomavirus 16 (HPV 16) capsids, efficiently induced the outgrowth of papillomas as early as 3 weeks after application to abraded skin on the muzzles and tails of athymic NCr nude mice. High concentrations of virions were extracted from homogenized papillomatous tissues and were serially passaged for >10 generations. Neutralization by L1 antisera confirmed that infectious transmission was capsid mediated. Unexpectedly, the skin of the murine back was much less susceptible to virion-induced papillomas than the muzzle or tail. Although reporter pseudovirions readily transduced the skin of the back, infection with native MusPV1 resulted in less viral genome amplification and gene expression on the back, including reduced expression of the L1 protein and very low expression of the L2 protein, results that imply skin region-specific control of postentry aspects of the viral life cycle. Unexpectedly, L1 protein on the back was predominantly cytoplasmic, while on the tail the abundant L1 was cytoplasmic in the lower epithelial layers and nuclear in the upper layers. Nuclear localization of L1 occurred only in cells that coexpressed the minor capsid protein, L2. The pattern of L1 protein staining in the infected epithelium suggests that L1 expression occurs earlier in the MusPV1 life cycle than in the life cycle of high-risk HPV and that virion assembly is regulated by a previously undescribed mechanism.

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Section: Discussionsupporting

confidence: 86%

Section: Discussioncontrasting

confidence: 64%

mentioning

confidence: 70%

mentioning

confidence: 99%

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Characterization of Mus musculus Papillomavirus 1 Infection In Situ Reveals an Unusual Pattern of Late Gene Expression and Capsid Protein Localization

Handisurya

Day

Thompson

et al. 2013

J Virol

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“…MmuPV1 was then genetically characterized, and the complete genomic DNA (7510 bp) was cloned and published . MmuPV1 inocula were obtained from cell-free extracts derived from original papillomas and shown to be capable of infecting laboratory mice (Joh et al, 2012). In the study, MmuPV1 showed high competence for tumor development on cutaneous tissue, muzzle, tail, and dorsal skins of athymic B6.Cg-Foxn1 nu/nu mice.…”

Section: Introductionmentioning

confidence: 99%

MmuPV1 infection and tumor development of T cell-deficient mice is prevented by passively transferred hyperimmune sera from normal congenic mice immunized with MmuPV1 virus-like particles (VLPs)

Joh

Ghim

Chilton

et al. 2016

Experimental and Molecular Pathology

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Systematic Evaluation of Skin and Adnexa in Mutant Laboratory Mice

2014

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The skin and its adnexa, hair and nails, are one of the easiest organ systems to evaluate as it is the most accessible. However, mice are small, have lots of very fine hair of multiple types, and while major abnormalities are obvious, subtle ones, or the basis for these defects can be difficult to define. To assist in outlining basic approaches to evaluating mice clinically as well as microscopically with the help of a pathologist, methods are provided here that are used routinely in many laboratories. The mouse is a very useful mammalian model system for studying normal and abnormal (disease) development and there is a high degree of correlation with not only human biology and medicine but that of most other mammalian species. Utilizing basic approaches standardizes analysis and provides quality samples for analysis.

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Molecular diagnosis of a laboratory mouse papillomavirus (MusPV)

Cited by 35 publications

References 28 publications

Characterization of Mus musculus Papillomavirus 1 Infection In Situ Reveals an Unusual Pattern of Late Gene Expression and Capsid Protein Localization

Characterization of Mus musculus Papillomavirus 1 Infection In Situ Reveals an Unusual Pattern of Late Gene Expression and Capsid Protein Localization

MmuPV1 infection and tumor development of T cell-deficient mice is prevented by passively transferred hyperimmune sera from normal congenic mice immunized with MmuPV1 virus-like particles (VLPs)

Systematic Evaluation of Skin and Adnexa in Mutant Laboratory Mice

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