Molecular diagnosis of a vascular prosthesis infection, due to Propionibacterium acnes, by amplification and sequencing of 16S rDNA

Page, L. Le; Podglajen, Isabelle; Chemla, Eric S.; Mainardi, Jean–Luc

doi:10.1046/j.1469-0691.2003.00764.x

Cited by 8 publications

(4 citation statements)

References 8 publications

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“…However, different studies have reported the involvement of Propionibacteria spp. in serious infections, such as endocarditis, postoperative endophthalmic infections (Hykin et al , 1994; Jakab et al , 1996; Le Page et al , 2003), osteomyelitis, arthritis and sarcoidosis (Ishige et al , 1999). A recent case study (Schneider et al , 2000) documented the clinical sequelae of three incidents of platelet contamination by Propionibacterium acnes .…”

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confidence: 99%

Amplified‐fragment length polymorphism analysis of Propionibacterium isolates implicated in contamination of blood products

et al. 2005

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SummaryPropionibacterium acnes is implicated in most cases of bacterial contamination of platelet concentrates (PCs). To determine the source of contamination, amplified-fragment length polymorphism (AFLP) analysis was applied. This DNA fingerprinting technique was used to study the molecular relationship of 44 isolates derived from 22 PCs and 22 corresponding red blood cells concentrates (RBCs) from the same whole blood donations. The AFLP results together with sequencing analysis of the 1200 bp of the 16S ribosomal RNA gene revealed the existence of three main groups: two groups (groups 2 and 3) (55%) consisted of isolates that did not originate from skin flora and another group (group 1) (45%) comprised bacteria belonging to the skin flora. This latter group showed complete homology with reference strains of P. acnes. Therefore these isolates can be considered as P. acnes strains. In contrast, contaminants from groups 2 and 3 were shown to be molecularly unrelated to the P. acnes found on the skin surface. The AFLP is reproducible and gave invaluable information about the nature of Propionibacteria contaminating PCs. To gain more insights into the source of contamination, this technique could be exploited in further studies to determine the molecular relationship of different bacteria commonly found in blood products.

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Amplified‐fragment length polymorphism analysis of Propionibacterium isolates implicated in contamination of blood products

et al. 2005

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“…[3][4][5][6][7][8][9][10][11][12][13] Eight of these cases had previous cardiac operation history and prosthesis implantation. The duration between surgery and presentation of infective endocarditis varied from 3 weeks to 4 years.…”

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Endocarditis caused by Propionibacterium acnes: an easily ignored pathogen

Pan

Wang

Hsueh

et al. 2005

Journal of Infection

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“…PCR amplification and sequencing of the 16S rRNA gene have been used successfully in previous studies to detect the slow-growing P. acnes in vascular prosthesis infections and infective heart valve endocarditis [16, 17]. In these cases, no bacteria were observed in the Gram stain, and no growth was observed after culturing.…”

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confidence: 99%

“…They reported improvement in the percentage of identification (up to the species level) and in the detection time. This combined method might be superior to culture methods in the diagnosis of bacterial infections if the patient is receiving empirical antibiotics, if the infection is caused by bacteria with unusual growth requirements, or if the pathogen is of an unexpected strain [16]. …”

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confidence: 99%

A Case of Brain Abscess Caused by Propionibacterium acnes 13 Months after Neurosurgery and Confirmed by 16S rRNA Gene Sequencing

et al. 2011

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Propionibacterium acnes is a gram-positive anaerobic bacillus and a normal inhabitant of the skin. Although it is often considered a contaminant of blood cultures, it can occasionally cause serious infections, including postoperative central nervous system infections. Here, we report the case of a 70-yr-old man who developed a large cerebral abscess caused by P. acnes 13 months after neurosurgery. Immediate gram staining of the pus from his brain revealed the presence of gram-positive coccobacilli. However, colony growth was observed only after 5 days of culture. Therefore, we performed 16S rRNA gene sequencing of the pus specimen. The isolate was identified as P. acnes. The colonies developed 9 days after the initial culture. The API Rapid ID 32A test (bioMérieux, France) was performed using a colony, but an unacceptable profile was obtained. Then, the pus was transferred into the enrichment broths of the BACTEC FX (Becton Dickinson, USA) and BacT/Alert 3D (bioMérieux, Organon Teknika, USA) systems, but only the BACTEC FX system could detect growth after 5 days. We performed 16S rRNA gene sequencing and API Rapid 32A profiling with a colony recovered from Brucella agar, which was inoculated with the microbial growth in the enrichment broth from the BACTEC FX system. The organism was identified as P. acnes by both methods. This case suggests that 16S rRNA gene sequencing may be a useful alternative for identifying slowly growing P. acnes from specimens that do not show growth after 5 days of culture.

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Molecular diagnosis of a vascular prosthesis infection, due to Propionibacterium acnes, by amplification and sequencing of 16S rDNA

Cited by 8 publications

References 8 publications

Amplified‐fragment length polymorphism analysis of Propionibacterium isolates implicated in contamination of blood products

Amplified‐fragment length polymorphism analysis of Propionibacterium isolates implicated in contamination of blood products

Endocarditis caused by Propionibacterium acnes: an easily ignored pathogen

A Case of Brain Abscess Caused by Propionibacterium acnes 13 Months after Neurosurgery and Confirmed by 16S rRNA Gene Sequencing

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